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OfflineJahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 3 months
Cloning
    #18938362 - 10/05/13 11:09 PM (10 years, 3 months ago)

three pieces of the middle of the cap were taken and put in a quart mason jar with nothing in it.
It was just being stored untill agar was obtained but mycelium already started to grow.

What is the next step to do now? because being low on spores is not good:frown:

If It is put in a half pint jar with pre mixed vermiculite; brown rice flower; and water will the mycelium spread?


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Cloning [Re: JahLambsbread]
    #18938388 - 10/05/13 11:19 PM (10 years, 3 months ago)

Was the quart jar sterilized and kept under aseptic conditions when you put the tissue in it?

If not, it needs to be moved to agar or you will probably just contam your PF cake.

If so...how do you plan to move the tissue or mycelium to the half-pint jar in way that does not introduce contams?


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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OfflineJahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 3 months
Re: Cloning [Re: FrankHorrigan]
    #18939219 - 10/06/13 08:13 AM (10 years, 3 months ago)

If so...how do you plan to move the tissue or mycelium to the half-pint jar in way that does not introduce contams?

UHH that's why I am asking and no I didn't sterilize it but it was a brand new jar out of the plastic so I am sure it's fine.

Maybe I will just dump them into the sterilized half pint.

How do you make an agar plate?


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Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Cloning [Re: JahLambsbread]
    #18939255 - 10/06/13 08:32 AM (10 years, 3 months ago)

Contamination Nation.

There are a thousand ways to make agar. Type agar into the search function on the main page and like 20 links pop up with all kinds of agar recipies. Search the posts in Mush Cult too. Also, find RR's home page and watch his sample videos. You have a lot of learning to do, son.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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