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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
I found my source of contamination!
    #18937103 - 10/05/13 05:40 PM (10 years, 4 months ago)

:awesome:
I was having a bad run for a while.  Couldn't figure it out.  I realized that my first run in front of my flowhood, being 100 percent no contam, was before I installed a screen on the front of my flowhood. I placed the screen because I saw a tek where someone did that to insure not damaging the hood by accidentally bumping into it.  Figured it was a good idea. My blower is a bit on the lower side.  I prolly should have got a one step up more powerful blower in retrospect. I'm thinking, with the screen on it made for less of a laminar flow.  That or the screen itself habored contaminants. I always wiped the screen with alcohol before working in front though so I honestly think it just cut down the airflow, and/or made eddies.  Bottom line is since I took it off, I've had a good run and back to no contams. Just an FYI if anyone else has this issue, and I'm psyched that I'm back baby!  Lol. I was worried for a while my filter itself went bad.  Maybe collected some mold or something. But nope.  Minus the screen I'm lookin good.  Just thought I'd share. :cheers:


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflineStromriderM
This must be the place
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Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say... Flag
Last seen: 14 hours, 38 minutes
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Re: I found my source of contamination! [Re: DeadPhan]
    #18937150 - 10/05/13 05:50 PM (10 years, 4 months ago)

Fuck yeah :rockon:


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
Re: I found my source of contamination! [Re: Stromrider]
    #18937182 - 10/05/13 05:56 PM (10 years, 4 months ago)

All spring/summer I assumed it was bad spawn as I was doing g2gs.  Then when I started with agar n realized I had a problem stemming from the hood itself. For a minute I thought nothing of the screen and thought my hood was flawed in general. :crankey:  until I did another lighter test and realized the flow was low.  Pulled back a tad of the screen, and the lighter bent a lot more.  So I ripped off the whole thing.  Did some agar afterwards and, bam!  :dancingshroom:


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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Offlineba87
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Registered: 09/06/13
Posts: 37
Loc: Canada Flag
Last seen: 9 years, 6 months
Re: I found my source of contamination! [Re: DeadPhan]
    #18937251 - 10/05/13 06:11 PM (10 years, 4 months ago)

Nice and congrats!


I will be using this "lighter test" when I do my own DIY lam hood very soon.

Really happy for you. Nothing gets one down more than continuous contams.


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OfflineEarthBounded
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Registered: 03/02/13
Posts: 326
Loc: North america
Last seen: 9 years, 1 month
Re: I found my source of contamination! [Re: ba87]
    #18937622 - 10/05/13 07:46 PM (10 years, 4 months ago)

Good to know im about to buy a flow hood, this is good info. Where's the best deal on a flow hood?

psillyshroomer, beautiful fucking mono, want to send me some of those genetics that is what I am chasing right now. I will trade you Krystal Coles signed book BAHAHAHA


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Kundalini and Chakra way of life....

Never give up!


Edited by EarthBounded (10/05/13 07:49 PM)


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Offlined9c6
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Registered: 07/02/13
Posts: 632
Last seen: 7 years, 5 months
Re: I found my source of contamination! [Re: ba87]
    #18937719 - 10/05/13 08:12 PM (10 years, 4 months ago)

Quote:

ba87 said:
Nice and congrats!


I will be using this "lighter test" when I do my own DIY lam hood very soon.

Really happy for you. Nothing gets one down more than continuous contams.



Nice avatar :wink:

that's great OP!


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Edited by d9c6 (10/05/13 08:13 PM)


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OfflineKizzle
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Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 17 hours
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Re: I found my source of contamination! [Re: d9c6]
    #18937787 - 10/05/13 08:27 PM (10 years, 4 months ago)

A screen would cause all kinds of turbulence. It seems pretty likely that was it.


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InvisibleSpitballJedi
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Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
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Re: I found my source of contamination! [Re: DeadPhan]
    #18937885 - 10/05/13 08:50 PM (10 years, 4 months ago)

:congrats::wow:


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The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Edited by SpitballJedi (10/05/13 08:51 PM)


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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OG Cultivator
Re: I found my source of contamination! [Re: SpitballJedi]
    #18938944 - 10/06/13 05:07 AM (10 years, 4 months ago)

Anything which comes between the face of the flowhood and your sterile media will allow contamination to enter the airstream.  This is also why it's so important to work right up against the filter, perhaps only 1" away.  You also have to keep your fingers from getting between the flowhood and your petri dishes, even with gloves.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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