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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Make syringe today and let spores rehydrate or 2morrow and just use?
#18936271 - 10/05/13 02:08 PM (10 years, 3 months ago) |
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Prepping my rye berries now so I can PC them and let them cool over night. Have a P. Galindoi print I'll be working with and was curious to see what people thought about the whole rehydration of spores concept.
I planned on just making a syringe tomorrow when I threw some of the spores on agar. Would it really be worth my time to go ahead and make the syringe today so the spores have a bit of time to rehydrate themselves or would throwing one together 2morrow then using it afterwards really make much of a significant difference?
I assume if I allowed them a bit of time to rehydrate they would show sign of growth sooner however considering I'm shooting for stones in this particular grow a few extra days will go unnoticed.
What cha think?
Mosey
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Make syringe today and let spores rehydrate or 2morrow and just use? [Re: Mosey3012]
#18936291 - 10/05/13 02:12 PM (10 years, 3 months ago) |
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I'd make it today and let the spores rehydrate a bit.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Re: Make syringe today and let spores rehydrate or 2morrow and just use? [Re: mushmagic]
#18936391 - 10/05/13 02:40 PM (10 years, 3 months ago) |
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The print was made 8/2013*
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 2 months
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Re: Make syringe today and let spores rehydrate or 2morrow and just use? [Re: Mosey3012]
#18936534 - 10/05/13 03:27 PM (10 years, 3 months ago) |
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They shouldn't need to be rehydrated. Also, if you're using agar, why make a syringe?
Just do spores to agar and go from there. A 3 month old print shouldn't need to be reydratrd for it to terminate. I have a 8 month old print that germinates in 4 days with just the spores to agar
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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Re: Make syringe today and let spores rehydrate or 2morrow and just use? [Re: Blake_Shroom]
#18936789 - 10/05/13 04:33 PM (10 years, 3 months ago) |
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For some reason I've had shit luck with getting straight spores to germinate onto agar. Moving a colonized grain or doing a clone has been no issue at all though. I've just been using a LME and agar mixture using Doc_T's tek:
using http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981
What particular mixture do you use for your agar? Is there one you've found that work especially well for spore to agar?
I figured Id make a syringe and see how that worked out and throw some onto agar as well to see if it'll grow out for me. I guess I like to over prepare myself... If the agar grows out then I'm more then likely going to make another set of jars to put aside using that and just keep that alive and moving while I work my way down to an iso if possible.
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 2 months
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Re: Make syringe today and let spores rehydrate or 2morrow and just use? [Re: Mosey3012]
#18936826 - 10/05/13 04:41 PM (10 years, 3 months ago) |
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I use mea. But golandi doesn't like it. It still works, bit doesn't grow as fast
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