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Offlineoohazard
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Fruiting chamber for 2 weeks but not pinning
    #18935399 - 10/05/13 09:44 AM (10 years, 3 months ago)

Hi all

I have been looking for answer on the board but since even after reading them I am not sure what to do I decided to create another post.

The incubation went really smooth, after less than 3 weeks the mycelium had fully colonized the vermiculite+brow rice floor substrate.

The main reason for this fast colonization was that I was able to maintain a good and stable temperature for them (80F) (I used a aquarium heater and put the incubation box into another one (filled with water)

Then after waiting 5/7 days after full colonization I created my fruiting chamber. I soak the cakes in water for 24-26 hours then rolled them in vermiculite and finnaly put them in the fruiting chamber.


Waiting 1 week and half I started worrying since none of the cake (5 of them) were pinning. They were fluffing a lot and I took that as an clue for an healthy micelium but I wanted to see them pinning :smile:.
I looked in the forum and found some answer mentionning a possible issue with temperature and a lack of air.

I started to put light (CLF) on during the day since the location in the kitchen (on top of the fridge) is not so bright and I stopped giving regular water spray as I read in some post someone doign that and getting the mushroom to pin.

2 days later I noticed a very small mushroom fruiting (less than 1 cm) but it did not continue to grow, mycelium seemed to start covering it.

the temperature in the fruiting chamber vary from 65 to 75 (sometime 80) but mostly arround 70

I am now afraid the fruiting chamber will start to dry (the perlite floor is not wet anymore)

Any idea what should I do ?

Someone suggested me I put one of the cake in the freezer for 1-2 hour to make it pin, is it a good idea?

I read also that I could make a new casing for the cakes (since all have fluffed so much that the mycelium covered the casing.

ANy help would be very appreciated, thanks



Edited by oohazard (10/05/13 09:46 AM)


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OfflineeLeSDenes
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #18935444 - 10/05/13 09:58 AM (10 years, 3 months ago)

Water the perlite, give them a lil bit more fresh air by hand fanning with the lid. Please forget about putting your cakes in the freezer


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InvisiblePastywhyteMDiscord
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #18935517 - 10/05/13 10:22 AM (10 years, 3 months ago)

Quote:

oohazard said:
I looked in the forum and found some answer mentionning a possible issue with temperature and a lack of air.




Too little FAE is no good, FAE and evaporation are primary pinning triggers :shrug:


Quote:

oohazard said:
I stopped giving regular water spray as I read in some post someone doign that and getting the mushroom to pin.




Unless you were majorly overmisting to begin with this will not help. Mushrooms need lots of water and high RH to pin.


Quote:

oohazard said:
I am now afraid the fruiting chamber will start to dry (the perlite floor is not wet anymore)




Then remoisten the perlite, its not hard :shrug:


Quote:

oohazard said:
Someone suggested me I put one of the cake in the freezer for 1-2 hour to make it pin, is it a good idea?




:nojustno: Cubes do not require nor do they benefit in any way from a cold shock


Quote:

oohazard said:
I read also that I could make a new casing for the cakes (since all have fluffed so much that the mycelium covered the casing.




I wouldn't, just remoisten your perlite and get your misting fanning schedule down. Mist then fan. No need to fan like mad, just enough to change out the air. Mist until the cakes glisten. If they are still glistening when you go to mist again, hold off until they are not.

From looking at your pics it appears that you do not have your holes drilled every 2 inches as is recommended. Do you have holes on all six sides? If these things are not done properly you can be sure the chamber is not functioning as intended. I recommend ensuring your chamber is built properly, check your mist and fan schedule, then smoke a bowl and chill the fuck out :lol:


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OfflineBLINKfan420
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Re: Fruiting chamber for 2 weeks but not pinning [Re: Pastywhyte]
    #18935605 - 10/05/13 10:51 AM (10 years, 3 months ago)

Arial myc is due to lack of FAE and evaporation.  I agree to re-hydrate your perlite and then fan a little more.

It does look like you could use a few more holes in your SGFC.  Also, its not flush against the walls in the corner is it? Some space on all six sides helps/if isn't essential.



BLINK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: BLINKfan420]
    #18935832 - 10/05/13 12:09 PM (10 years, 3 months ago)

Thanks all for your advices!

I did not put enough hole, I ll do some more tomorow.
I never fanned much before. I ll do that more.

For rehydrating the perlite, should I remove the perlite and do as I did the first time. Or can I just pour some water in the chamber directly?

What about recasing ?
Is it fine if I do not?

Cheers


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InvisiblePastywhyteMDiscord
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Re: Fruiting chamber for 2 weeks but not pinning [Re: BLINKfan420]
    #18935907 - 10/05/13 12:36 PM (10 years, 3 months ago)

OP just so your aware, aerial mycellium is usually due to a lack of FAE. Fanning will not help with FAE it simply promotes evaporation (which is a pinning trigger). It won't matter if the cakes are close to the walls but, if you place the cakes closer together that will help promote a better microclimate between the cakes.


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: Pastywhyte]
    #18935942 - 10/05/13 12:45 PM (10 years, 3 months ago)

Hi Pastywhyte

When I think fanning, I am thinking about me using the lid to move the air. It should create some FAE, right?

to be clear when you say fanning won't help with FAE you mean the air moving inside the chamber without moving out of it, right ?

They are not close to the wall but they might be to far from each other, I ll change that

Thanks


Edited by oohazard (10/05/13 12:46 PM)


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InvisiblePastywhyteMDiscord
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #18935998 - 10/05/13 12:58 PM (10 years, 3 months ago)

When I say fanning won't help with FAE what I mean is that cubes need between 1-3 air exchanges per hour. Unless you plan to be fanning around the clock and not sleep, fanning will not be a substitute for the constant FAE that would be provided by a properly built SGFC. Fanning will evaporate the water from your misting and will move in some unsaturated air to keep a little evaporation going for a bit. This is a pinning trigger.

Hope that explains it.


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: Pastywhyte]
    #18938637 - 10/06/13 01:11 AM (10 years, 3 months ago)

Ok, I understand, so I definitively need to make more holes

Thanks again,


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OfflinePussyFart
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #18938729 - 10/06/13 02:04 AM (10 years, 3 months ago)

1/4 inch holes every 2" in a grid pattern on all 6 sides...


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: PussyFart]
    #18962126 - 10/10/13 11:38 PM (10 years, 3 months ago)

Hi!,

following your advices I started to see fast growth.

But only few pin.

Also the first to pin looks quite big now but have some green around the head and some white dot

Should I harvest them , are safe to eat ?

Thanks



Edited by oohazard (10/11/13 12:49 AM)


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #19003141 - 10/20/13 09:30 AM (10 years, 3 months ago)

Hi, a follow up

After I harvested the  mushroom shown in the second set of pictures I did not get anything else.

There are few pin but nothing grow.

Here some new photos. I am not sure what i am doing wrong now. There is even a mushroom that does not look normal. Another does not grow anymore (1cm).

I am now ventilating and misting 3 times a day.

Any advices?



Thanks


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OfflineStromriderM
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #19003170 - 10/20/13 09:41 AM (10 years, 3 months ago)

Perhaps you should dunk them. Pick one up and see how heavy it feels. You want it to feel close to the weight it was after you initially dunked them. If they are really light then re-dunk them


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: Stromrider]
    #19004044 - 10/20/13 01:38 PM (10 years, 3 months ago)

Hi

I just did it,

I noticed there was some gree/blue fungus at some places on the surface so I cut almost all the side of the cakes. They are smaller now but I hope cleared of all other fungus.

I put them in water. Tomorow I ll roll them in vermiculite and put in teh fruit chamber on top of clean aluminium foil. I hope they will survive and grow :smile:

I ll post an update whatever happen

Thanks


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Offlineoohazard
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #19088583 - 11/05/13 03:55 AM (10 years, 2 months ago)

After putting them back into the fruiting chamber 2 weeks ago, the mycelium started to grow again on top of the vermiculite(casing) but no mushrooms pin formed.

What is wrong with my setup?

3 times a day I misted and fanned them.

Since I have more holes to teh chamber on top of some already made holes they do not form a uniform grid and there might be more holes than it should. Could it be the reason?

Also my fruiting chamber is quite big, I heard somewhere that the ideal size is 12 inches with 5 inch of perlite.

Should i try again or should I throw them away ?

One last thing: could it be because of temperature?
The temperature since last 2 weeks has falled quite a lot and now it never reach 70F or more.

What the best way to achieve constant temperature without drying them?
My home is quite cold nowadays and I plan to start using the radiators for some time of the day/night.

Can I use this fact for my mushroom?


Thanks for everything


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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #19088649 - 11/05/13 04:35 AM (10 years, 2 months ago)

They look oversaturated in those pictures. Also I am curious about what you mean by cutting off the blue green fungus on the outside of the cake.

Remember that pinning is triggered by evaporation. Mist 2 Times- Fan 4-5. This way your cakes have a reaction and produce little mushrooms. Once they start to pin you can increase your misting to 2-3 times a day.


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OfflineParty-boy
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Re: Fruiting chamber for 2 weeks but not pinning [Re: oohazard]
    #23323067 - 06/08/16 06:52 PM (7 years, 7 months ago)

Hey, that picture u put up of the digital hydrometer u had was reading false humidity probly. I got mechanical one and trust it to with 5%


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