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SgtGanja
CEO


Registered: 09/03/13
Posts: 134
Loc: United States
Last seen: 8 years, 11 months
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Proper Pasteurization
#18930944 - 10/04/13 12:11 PM (10 years, 4 months ago) |
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As I'm sure most of you will probably agree, proper pasteurization is definitely the best route to avoid the possibility of contamination and what not for spawning to bulk. I will be using a coir/verm/gypsum substrate. I am aware of Franks proper pasteurization tek using jars but do not have any extra jars available at the moment(if need be I can pick up some more). I am also aware of Evilmushrooms proper pasteurization tek using bags but would like more clear and up to date info. Most of the information that I can find regarding properly pasteurizing bulk substrate inside of polypropylene bags(or something of the sort) all seems to be from several years back or quite ambiguous so I am looking for some clarification.
This is the process I am thinking: 1) Mix substrate in bucket and bring to field capacity. 2) Load substrate into bags and either zip-tie or seal the bags closed. 3) Load bags in PC with 2-4 inches of water, stick thermometer in bag and seal it with micropore tape(or something of the sort). 4) Turn stove on medium heat until internal temp rises to about 100. 5) Turn heat off, put lid on to make a seal, but not turning it as to create pressure. Once internal temp reaches 140, start timer for one hour.
First I would like to ask, will this even work? If so, what adjustments or tips would you give to make this process most efficient? Would I need to put anything under the bags in the PC aside from the rack that is already in there? If I were to use zip-ties, could I stuff the small opening that will be left with polyfill and put the thermometer through that?
Peace and thanks!    Sgt
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Proper Pasteurization [Re: SgtGanja]
#18931050 - 10/04/13 12:36 PM (10 years, 4 months ago) |
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Sounds perfect to me 
No need for the seal around the thermometer and you may want to use more water.
Here's my pictorial
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Dark76


Registered: 07/14/13
Posts: 121
Last seen: 5 years, 9 months
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I was just going to point him in your direction, since you added an entire section on bags.
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SgtGanja
CEO


Registered: 09/03/13
Posts: 134
Loc: United States
Last seen: 8 years, 11 months
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Quote:
FrankHorrigan said: Sounds perfect to me 
No need for the seal around the thermometer and you may want to use more water.
Here's my pictorial 
Dang, I don't know how I overlooked that Thanks!
Additionally, when you say filter patch bag; is that like a regular oven bag from the grocery store, autoclave, polypropylene, or just from a vendor? I don't wanna get the wrong bags!
Also, you just put the thermometer directly through the bag and into the substrate? Won't this create a way for moisture to get in, or is that what the filter patch is for?
Lastly, are those bags reusable?
Thanks for the help! Sgt
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Gretchenmeister
Starbeing/Psilocybin Savant



Registered: 07/23/05
Posts: 1,032
Loc: From the Stars
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Re: Proper Pasteurization [Re: SgtGanja]
#18933036 - 10/04/13 08:23 PM (10 years, 4 months ago) |
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Because I like to do large amounts at a time, and I don't like pastuerizing over a heat source, this is my preferred method.
1. Place bulk ingredients in a LARGE tub or cooler. (COIR OR POO RECIPE)
2. Heat Several Large Pots of water on the stove to 175F
3. Add a couple gallons of hot water to the substrate to thoroughly hydrate it, then mix well. Coir will take several minutes to fully expand.
4. Add water from the stove that was preheated to 175F. Add enough water so that the substrate is floating in the water.
5. Check the temperature of the substrate/water bath. Add hot water as needed until the temperature is exactly 170F.
6. Close the lid and wait one hour.
7. Recheck the temperature. If the temperature is above 160F after one hour, close the lid and allow the mixture to cool enough to be handled. This usually takes 2-4 hours.
If the temperature is below 160F, add more hot water to bring the temp back to 170F, close the lid and start the time again.
8. Put your gloves on. Dip the substrate out of the water bath and allow the excess water to drain back into the tub. Then squeeze each handful until it is to field capacity. Generally it is squeezed until barely a drop of water can be squeezed out by hand pressure.
9. Put in a clean rubbermaid and allow to cool before spawning.
Note: Substrate can be prepared up to a week ahead of time, and kept in a sterilize or rubbermaid with a filter patch. Pasteurized manure substrates can be frozen and thawed a couple days prior to use without repasteurization.
-------------------- What's wrong with folks? Point your IRC client to irc.socialirc.com, port 6667, #cultivation and #shroomery for live chat with like minded hobbyists and connoisseurs. Mush Porn
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Proper Pasteurization [Re: SgtGanja]
#18933080 - 10/04/13 08:32 PM (10 years, 4 months ago) |
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Quote:
SgtGanja said: 3) Load bags in PC with 2-4 inches of water, ...
I'm just being nitpicky. But I have found that saying "PC" confuses a lot of people. People are not smart. They confuse it with "PCing" as in sterilization.
I know what you mean, but maybe just say "large enough pot" or something. Just a suggestion for the noobs who might be reading. It may prevent a barrage of posts like "I thought substrate only had to be pasteurized, not PCed."
Nice job though
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Use more than 2-4 inches of water too. Once you get the substrate bag in, add water until just before it floats. Having more water in contact with the bag will heat your substrate material more evenly. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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SgtGanja
CEO


Registered: 09/03/13
Posts: 134
Loc: United States
Last seen: 8 years, 11 months
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Gretchen, that tek seems like a novel idea. May have to try it out some day.
Quote:
SpitballJedi said:
Quote:
SgtGanja said: 3) Load bags in PC with 2-4 inches of water, ...
I'm just being nitpicky. But I have found that saying "PC" confuses a lot of people. People are not smart. They confuse it with "PCing" as in sterilization.
I know what you mean, but maybe just say "large enough pot" or something. Just a suggestion for the noobs who might be reading. It may prevent a barrage of posts like "I thought substrate only had to be pasteurized, not PCed."
Nice job though
I actually thought about this as I was posting it but I figured eh, why not? But that clarification definitely helps for those that have read.
Quote:
RogerRabbit said: Use more than 2-4 inches of water too. Once you get the substrate bag in, add water until just before it floats. Having more water in contact with the bag will heat your substrate material more evenly. RR
Ah, well that makes sense. Thanks for the knowledge RR!
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
RogerRabbit said: Use more than 2-4 inches of water too. Once you get the substrate bag in, add water until just before it floats. Having more water in contact with the bag will heat your substrate material more evenly. RR
Agreed, this is exactly what I do.
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