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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Tampanensis fruit trial: first flush pins update (photos)
#18927959 - 10/03/13 08:40 PM (10 years, 3 months ago) |
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Hi everyone,
First method:
4 months ago I used P tampanensis wedges to inoculate 5 quarts of strait rye. My current tampanensis cultures originate from 5 colonised grains that I rescued from my first and only master that contaminated.
Rye was sterilized at 15 psi for 90 mins; for jar lids I used large synthetic filter discs (1 per jar) locked in with the metal ring...and covered with aluminium foil). Works fine.
I gave them a shake at 10-20% and waited.
Conditions: tropical weather, hot and humid. 20-32 Celcius.
I harvested 3 jars at 3.5 or 4 months old.
Result: each jar yielded between 60 and 70 gr of fresh sclerotia. Potency is good. Visual at first then more clear and mental but very interesting 
I was disappointed with the yield and most sclerotia were small....took me hours.
I spawned a quart of grains to pasteurized cow manure. It is colonizing slowly.
Method 2 (way better):
I followed a great tek borrowed from someone on this website but I can't find the thread anymore, apologies ...
Here is the original tek that I kept on a side note:
"My Recipe For 7 Quart Jars: 11 Cups Dry Rye Berries 2 1/2 Cups Dry Grass Seed 10 cups Strong Coffee 2 tbsp Gypsum (Not necessary, but helps a lot)
Cover everything with enough water so that there is 3" of water over the rye berries. The grass seed will float at first, don't worry, this is normal.
Add the 8-10 cups of strong coffee, turn burner on very low, let soak for 3 hours while stirring every 15 to 30 minutes. You will know the rye berries are done when you can pinch all the way through one with your fingernail, but they do not smash when pinched between your fingers. Firm but moist on the outside, wet on the inside.
I then dump the rye into a strainer, pouring as much water/coffee as I can out before dumping the substrate into the strainer, shake the strainer around to drain as much water off as I can, and then let the substrate sit for a few hours. It takes longer to dry this way than simmering, but you burst no kernels using this method and it allows you to soak all of the substrate at once instead of doing the rye berries and grass seed separately.
Once the rye berries no longer stick to your hand when you lay your palm on them, they are ready to go into the pressure cooker. Load them into the jars and shake well with a solid lid before putting the new GE lid on the jar. Smacking the bottom of the jar against your palm works better than hitting it against something hard to knock the grass seed and rye berries off the lid and glass.
Load your filled jars with their new GE lids attached into your pressure cooker and cover them with aluminum foil. You need to keep the filter squares dry, or so I hear. I've never had a contam even when they got wet, but it's better to be safe. Pressure cook them at 15psi for 90 minutes. When done, your rye berries will look golden brown and dry/barely moist on the outside."
[gradient:#9C223D,#]IT WORKS WONDER!! This tek should be included in the the growing info tabs as it is so easy.[/gradient]
My report:
Inoculated 7 or 8 jars prepared as per tek above with random sectors from the same plates I used for the previous batch.
NO SHAKE this time.
Faster colonisation sclerotia started to form at 30% colonisation! Some where already big like a marble by 100%...
After 1 month the truffles were way bigger than my mature straight rye truffles...crazy. And more numerous. Substrate doesn't dry out as much either for some reason.
I estimate the yield will be three times higher than straight rye. They are still growing so I will leave them a bit longer but will harvest a test jar this week end just because I'm curious.
I will post photos around tomorrow and report on second batch yield.
I then plan to spawn grains to coir/verm/gyps in a monotub.
Hope other people try this tek, nothing revolutionary but I think it is the best out there for ease and efficiency.
Spiral
Edited by Spiral Climber (10/28/13 11:55 PM)
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krunkmaster
Cannibal


Registered: 04/26/13
Posts: 117
Last seen: 2 years, 3 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#18928222 - 10/03/13 09:28 PM (10 years, 3 months ago) |
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sounds great, thank you! I will hopefully try this some day when I am more experienced.
-------------------- Thou shalt not kill my vibe
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#18928245 - 10/03/13 09:34 PM (10 years, 3 months ago) |
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Hey quick question since I'm planning on growing stones in the near future I was wandering how you can really tell if your truffle jar is contaminated since the pictures I've seen of fully colonized sterile truffle jars have some whites some yellows and some browns with the stone production and with cubes I know my jars are contaminated when I see any color other than white?
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: spaceman101]
#18947923 - 10/08/13 01:56 AM (10 years, 3 months ago) |
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I harvested 2 jars that were 3 months old jar grown with this tek of MS tampanensis(with process described above):
"My Recipe For 7 Quart Jars: 11 Cups Dry Rye Berries 2 1/2 Cups Dry Grass Seed 10 cups Strong Coffee 2 tbsp Gypsum
Harvested 100gr/jar. Well one jar I only got 80 gr from because the top got a recent contam and I discarded 25% of the content...including some nice looking sclerotia (the top 25%).
And it looks like they could have grown bigger...I have another 5 jars that I will harvest way later.
The sclerotia in small RGS plastic containers (4 months old) did pretty well and I found it easier to find and clean the truffles.
The overall aspect and size of sclerotia was by far nicer when grown in straight RGS or Rye/RGS/Gypsum/Coffee soak as compared to straight rye. Straight rye is just lame.
I've lost my SD card reader so cannot upload pics for now sorry, but they will come.
I also spawned the grains of 3 RGS small pp5 containers + about 2 quarts of grain to a coir (1 brick)/verm/gypsum monotub. It is colonizing nicely. I don't know yet if I'll fruit it or not, I think manure would be better for this purpose? I really want some prints..
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: spaceman101]
#18947935 - 10/08/13 01:59 AM (10 years, 3 months ago) |
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Quote:
spaceman101 said: Hey quick question since I'm planning on growing stones in the near future I was wandering how you can really tell if your truffle jar is contaminated since the pictures I've seen of fully colonized sterile truffle jars have some whites some yellows and some browns with the stone production and with cubes I know my jars are contaminated when I see any color other than white?
It naturally turns to strange colors (from white to beige to rust...) but if you have a contaminant you should be able to tell the difference (signs of a fight such as metabollites, or boundaries...). Also when you open your jar (wait at least 3 months) it should smell like mushroom. If the top layer is a bit dry just scrap it off and it should smell a lot better underneath. Best luck to you!
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#19049786 - 10/28/13 11:54 PM (10 years, 3 months ago) |
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Update with pins, first flush.
tek: after dunking grains in tap water, I spawned to various substrates. Some grains sat in my fridge simply on a plate with plastic wrap for nearly 2 weeks before spawning, and got ZERO contam 
casing layer: peat moss + verm. No particular ratio...seems to work anyway. Used Frank's pasteurization tek.
Cow manure/straw/verm/gyps/coir trays sitting in a monotub with perlite + water at the bottom:

Coir/verm/gypsum classic monotub:

This one started pinning before I introduced air exchange so I fruited it and there's not much casing layer cause I ran out and am not sure I can apply more now without hurting things. It's only pinning on the sides but looks like primordia forming under casing layer. However some fruits on the side have stalled...
For some reason I struggle to get enough humidity in this one and usually this monotub is perfectly dialed in. The manure trays generate more humidity??? Anyone has suggestions?
This is my final active grow. I'm happy to get to see some fruits and maybe keep a few prints
Let me know what you think
Edited by Spiral Climber (10/29/13 12:01 AM)
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#19049906 - 10/29/13 12:34 AM (10 years, 3 months ago) |
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I think you will have better success with a sgfc rather than the mono tub.
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: inski]
#19085386 - 11/04/13 01:21 PM (10 years, 2 months ago) |
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I'm actually away at the moment cannot post photos but all the projects exploded with tall, thin mushrooms. Caps are small though and not well shaped on manure. The coir ones looked a bit better shaped before I left.
I think GE is good, humidity could be higher though. At the end of the day, they're really just as easy as cubes.
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#19085454 - 11/04/13 01:39 PM (10 years, 2 months ago) |
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what are boundaries and also how exactly can you tell the difference from metabolites and the strange colored myc since all the pics I've seen of truffle growth seems to add more moisture to the jar somehow and in a lot of the pics most of the myc to me looks like it's covered in metabolites "I don't have the best eyes for myc in the world". How much rye grain did you use in each of the jars and since there's no reason to shake the grain up would it allow more truffles to grow if I use a little more than a cup of rye grain for each jar?
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#19093281 - 11/05/13 11:36 PM (10 years, 2 months ago) |
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Here are some photos of my fruits.
I can't get it to stay humid enough 
Some shrooms are printing but caps were very small and not well shaped.
Some fruits became very skiny suddenly...what is this due to? I hope it isn't some contaminant and they're just dry.
manure trays:

coir/verm/gyps monotub with thin casing:

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bootster

Registered: 02/22/11
Posts: 1,531
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber] 1
#19093421 - 11/06/13 12:10 AM (10 years, 2 months ago) |
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Looks like Myc trying to get through, or some cottony contam. Keep an eye on it. So far it looks good.
That mono is cased, right? I missed that part. 50/50 correct?
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: bootster]
#19093606 - 11/06/13 01:13 AM (10 years, 2 months ago) |
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The mono had left over casing spread thinly (peat moss / verm 50 50 roughly). I think the white cottony thing is just mycelium poping through because it is thin. Mushrooms grow of that cottony stuff. But the some shrooms look abnormally skinny. What causes this?
The mono only had 3 quarts of leftover grains after sclerotia was harvested (about 4 months old). It is about 2" deep which is a bit short but well...
Edited by Spiral Climber (11/06/13 01:14 AM)
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Spiral Climber
Happy Farmer


Registered: 02/16/13
Posts: 143
Last seen: 9 years, 11 months
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Re: Tampanensis trial: first results - straight rye vs rye/RGS/Gyps/cofee soak [Re: Spiral Climber]
#19105822 - 11/08/13 01:56 PM (10 years, 2 months ago) |
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Harvest update first flush all together: 110 gr fresh tampanensis fruits.
Couldn't get any to drop spores Hoping second flush will bring something printable
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