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OfflineCheebs
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First attempt at indoor cultivation using PF Tek (pictures)
    #18927027 - 10/03/13 06:06 PM (10 years, 4 months ago)

What's up guys, this will be my first post with shroomery. I have been using the message boards for a little while now for identifying and researching the very useful information available here. After reading over a few PF Tek guides I decided to give it a try myself. I have never grown Psilocybe Cubensis, or any kind of mushroom for that matter so this is a new process for me. I have although, gone "hunting" and taken home colonized samples of manure which surprisingly gave me a few small flushes when placed in a shaded area in my backyard.

I collected many spore prints from the Cubensis caps I found in local farms around me which are what I used to inoculate my jars with. I followed the basic materials instructions and picked up some:

1. Vermiculite
2. Brown Rice
3. 1/2 pint mason jars

STERILIZATION:

I already own a few rice cookers so I used them to sterilize the substrate mixture placed inside the jars with a layer of dry vermiculite on the top. My substrate was followed by the general measurements found on PF Tek guides here on shroomery. After about an hour and a half of steaming, I removed the jars and allowed them to cool down for a few hours until they were at room temperature.

INOCULATION:

This is where it gets tricky. I don't own any syringes and I couldn't find a pharmacy that sold them off the shelves nor was I going to ask for one to save myself from an awkward situation… So……I improvised.

I used toothpicks, which I heated over a candle for sterilization, and scraped and rolled them over the spore prints from the wild mushrooms I had collected until there was a significant amount sticking to the toothpick. I then stuck the end of the toothpick into each of the four holes on the top of my sterilized substrate jars making sure to twist them to get all of the spores to fall off. I could see the path along the inside of the glass where the toothpick had been inserted and tons of purple spores all around it. For the full pint jar I just dabbed a cotton swab to on the spore prints and directly applied it to the substrate with the lid of the jar open. I did this on four sides and in the center of the mixture.

There were many factors of the toothpick and cotton swab methods I used that led me to believe this experiment would be a complete failure due to contamination but it was my first attempt at cultivation and I was only interested in doing it as a trail and error run so that I could learn the procedure for my next, more efficiently planned attempt.

COLONIZATION:

After inoculating six 1/2 pint mason jars and one full pint jar using two layers of tin foil to cover the holes I screwed the lid ring on the jars tightly and placed them away in a dark spot with

After about a week I decided to take a look at the jars and to my surprise I found them to be roughly 25% colonized with no signs of contamination. The growth inside the jars was a white, stringy, almost spider web looking mycelium that seemed to be stretching outward from the inoculation points.

I began checking the jars every day or two noticing more and more growth each time. I read that shaking or tapping the jars lightly on an angle helped spread the mycelium faster so I did this a couple of times and it seems to have stimulated a faster spread of the white growth inside. The full pint jar did show a pure white mycelium growth with no abnormal coloring but I noticed it was growing at a slower rate than the half pint jars were. I later realized through researching online that this was due to a lack of gas exchange since I used a cotton swab to directly inoculate the substrate with the lid off rather than nailing holes in the lid. I corrected this by making four small holes in the lid with a sterilized nail and covered them with foil the same way I did for the other jars and the growth improved over the next few days still with no signs of contamination.

I am 99% positive that what I have growing inside the jars is mycelium and not some form of mold or another contaminant (see pictures), although this is only my first attempt at this and I can not confirm it. Maybe somebody can do that for me…

It's now been two weeks and a few of the cakes seem to be 100% colonized on the outsides. I'm going to let them sit for another five or six days to let them colonize the centers of the cakes completely if they haven't already and begin putting together my fruiting chamber. I'm leaning towards the standard storage container with holes drilled and soaked perlite at the bottom to keep the moisture levels up. If anyone could give me a few suggestion that would be great :smile:

Here are pics of the progress so far, ordered by progression…



Well hopefully everything goes well in the fruiting process, I'll update with pictures once I have them out of the jars and in the fruiting chamber. Thanks for reading!


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cheebs]
    #18927047 - 10/03/13 06:11 PM (10 years, 4 months ago)

Awesome first post! Nice work my friend. Welcome to our community. 5 shrooms coming your way


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OfflineCpt.Crunch
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Registered: 08/09/13
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Stromrider]
    #18927133 - 10/03/13 06:25 PM (10 years, 4 months ago)

Quote:

I used toothpicks, which I heated over a candle for sterilization, and scraped and rolled them over the spore prints from the wild mushrooms I had collected until there was a significant amount sticking to the toothpick. I then stuck the end of the toothpick into each of the four holes on the top of my sterilized substrate jars making sure to twist them to get all of the spores to fall off. I could see the path along the inside of the glass where the toothpick had been inserted and tons of purple spores all around it. For the full pint jar I just dabbed a cotton swab to on the spore prints and directly applied it to the substrate with the lid of the jar open. I did this on four sides and in the center of the mixture.




That is pretty crazy, I think you should invest in some syringes/needles though for the future, you can check out the shroomery sponsors and order them from there. Or maybe amazon or ebay has some on there.

You could have also added gpysum to your brf/verm/water mix to increase the yields of your cakes.

Good job though, that toothpick method is interesting. Usually less spores is better though because your jars are less likely to contam.


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Stromrider]
    #18927141 - 10/03/13 06:26 PM (10 years, 4 months ago)

Btw don't shake PF Tek jars just grain jars


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cpt.Crunch]
    #18927175 - 10/03/13 06:30 PM (10 years, 4 months ago)

Quote:

Cpt.Crunch said:
Quote:

I used toothpicks, which I heated over a candle for sterilization, and scraped and rolled them over the spore prints from the wild mushrooms I had collected until there was a significant amount sticking to the toothpick. I then stuck the end of the toothpick into each of the four holes on the top of my sterilized substrate jars making sure to twist them to get all of the spores to fall off. I could see the path along the inside of the glass where the toothpick had been inserted and tons of purple spores all around it. For the full pint jar I just dabbed a cotton swab to on the spore prints and directly applied it to the substrate with the lid of the jar open. I did this on four sides and in the center of the mixture.




That is pretty crazy, I think you should invest in some syringes/needles though for the future, you can check out the shroomery sponsors and order them from there. Or maybe amazon or ebay has some on there.

You could have also added gpysum to your brf/verm/water mix to increase the yields of your cakes.

Good job though, that toothpick method is interesting. Usually less spores is better though because your jars are less likely to contam.





I really doubt gypsum would benefit brf cakes or increase yeild. Did you read this somewhere I've never heard anyone using gypsum in PF Tek


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OfflineCheebs
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Registered: 09/29/13
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Loc: SoFla
Last seen: 10 years, 3 months
Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cpt.Crunch]
    #18927205 - 10/03/13 06:35 PM (10 years, 4 months ago)

It definitely was a crazy method of inoculation but it seems to me like it's been successful so far! : P

Great advice, I will give the gypsum a try for my next grow. And I've already placed an order with one of the sponsor sites here on shroomery for a few syringes of different strains so I'll be using them for my next set of jars :smile:


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OfflineCheebs
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Stromrider]
    #18927214 - 10/03/13 06:37 PM (10 years, 4 months ago)

Quote:

I really doubt gypsum would benefit brf cakes or increase yeild. Did you read this somewhere I've never heard anyone using gypsum in PF Tek




I've seen this a few times but never actually looked into it, it seems worthy of a try


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cheebs]
    #18927228 - 10/03/13 06:40 PM (10 years, 4 months ago)

Well I don't think it would hurt anything


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Offlinehihihi1717
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cpt.Crunch]
    #18927327 - 10/03/13 06:54 PM (10 years, 4 months ago)

Quote:



That is pretty crazy, I think you should invest in some syringes/needles though for the future, you can check out the shroomery sponsors and order them from there. Or maybe amazon or ebay has some on there.

You could have also added gpysum to your brf/verm/water mix to increase the yields of your cakes.

Good job though, that toothpick method is interesting. Usually less spores is better though because your jars are less likely to contam.




How would you recommend getting gypsum from drywall (just crumbling the drywall and removing the cardboard backing and front layer?) and adding it to the mixture? Also, what kind of ratio do you recommend for the verm:water:brf:gypsum ratio? And how effective is it at increasing yields? Is there anything that can go wrong with this additional substance?

I'm about to begin my next attempt at a grow in the coming week and am interested in gypsum, but know nothing about it beyond hearing that it helps. Thanks in advance,


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OfflineCpt.Crunch
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Registered: 08/09/13
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: hihihi1717]
    #18927356 - 10/03/13 06:58 PM (10 years, 4 months ago)

Quote:

How would you recommend getting gypsum from drywall (just crumbling the drywall and removing the cardboard backing and front layer?) and adding it to the mixture? Also, what kind of ratio do you recommend for the verm:water:brf:gypsum ratio? And how effective is it at increasing yields? Is there anything that can go wrong with this additional substance?

I'm about to begin my next attempt at a grow in the coming week and am interested in gypsum, but know nothing about it beyond hearing that it helps. Thanks in advance,





It's one tablespoon of gypsum for 5 pf tek jars, just mix the gypsum with  the BRF before adding it to the water/vermiculite. And I believe it increases yields by 10% i'll try to find the link.

Here is the thread which states 1 tablespoon of gypsum per 5 jars, and how gypsum increases yields for PF Tek.

http://www.shroomery.org/forums/showflat.php/Number/17540975#17540975


Edited by Cpt.Crunch (10/03/13 07:00 PM)


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Offlinehihihi1717
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cpt.Crunch]
    #18927374 - 10/03/13 06:59 PM (10 years, 4 months ago)

And this cannot harm the spores/mycelium in any way?


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OfflineCpt.Crunch
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: hihihi1717]
    #18927381 - 10/03/13 07:00 PM (10 years, 4 months ago)

No , it just makes the mixture more nutritious,  check the post I made before this one, I added the link into that post.


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Offlinehihihi1717
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cpt.Crunch]
    #18927428 - 10/03/13 07:06 PM (10 years, 4 months ago)

Thanks for the info!


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OfflineCheebs
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: hihihi1717]
    #18927595 - 10/03/13 07:31 PM (10 years, 4 months ago)

Any suggestions for fruiting? I'm planning on using a large transparent storage container with holes drilled and soaked perlite at the bottom.

I have heard of people using lights and fans but I feel like it isn't completely necessary… Would shaded light be adequate for fruiting? I will most likely keep the chamber in a closet or under a table where it won't be disturbed by people, pets, etc...


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Cheebs]
    #18927712 - 10/03/13 07:53 PM (10 years, 4 months ago)

Yeah just build you a sgfc.


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OfflineStromriderM
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Stromrider]
    #18927727 - 10/03/13 07:55 PM (10 years, 4 months ago)



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Offlineghostshadow422
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: Stromrider]
    #19067467 - 11/01/13 12:48 AM (10 years, 3 months ago)

I have 60 pf tek jars going now, 12 of then from a mss solution and the other 48 from LC. the ones I injected with spores some of them seems I didn't shoot enough into the sides of some of the jars, so about 8 of the mss jars are knly colonized on kne side. the sides that are colonized are showing extremely well developed rizomorphic growth. Should I just let these jars continue to grow until they colonize the other side of the jar? it's been about 20 days now at this point, and it seems like the myc has stopped "reaching" around to the other side of the jar during the past week or so. I'm intending to birth all 60 at once, so is it possible that I just birth them half colonized around the jar?


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OfflinecronicrFacebook
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Re: First attempt at indoor cultivation using PF Tek (pictures) [Re: ghostshadow422]
    #19067589 - 11/01/13 01:21 AM (10 years, 3 months ago)

Let them finish
Shake the syringe between jars
Check the date of the threadds your posting on
Stop jacking threads
And welcome to the boards


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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