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Offlinemushbecrazy
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agar culture from syringe?
    #18923421 - 10/02/13 09:11 PM (10 years, 4 months ago)

Can i start a agar petri with spore syringe? Pan cayan  or P. Cubensis. If so how. Ive got 15 petri dishes ready for some thing.


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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OfflinePussyFart
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923429 - 10/02/13 09:12 PM (10 years, 4 months ago)

Yes.

Inoculate each dish with a drop or 2 of solution and let germinate and colonize.


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Offlinerumfor69
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Re: agar culture from syringe? [Re: PussyFart]
    #18923443 - 10/02/13 09:14 PM (10 years, 4 months ago)

Yes, in fact you want to goto agar from spore.

it will give you the ability to grow a clean culture seems how spores are

never completely sterile. So you grow then clean first, cut and copy,

clean myce if the dish is contaminated on one side or something.


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: PussyFart]
    #18923460 - 10/02/13 09:17 PM (10 years, 4 months ago)

Sweet was hoping to turn this $30 pan cyan syringe into a infinite stock pile of mycelium. Lol  :shocked:


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: rumfor69]
    #18923488 - 10/02/13 09:23 PM (10 years, 4 months ago)

Quote:

rumfor69 said:
Yes, in fact you want to goto agar from spore.

it will give you the ability to grow a clean culture seems how spores are

never completely sterile. So you grow then clean first, cut and copy,

clean myce if the dish is contaminated on one side or something.






Never thought about that. So i grow it then cut clean pieces out and start new ones. Or start all.  from syringe. How long does a petri full last. How much do you throw in grain when done


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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OfflinePussyFart
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923498 - 10/02/13 09:25 PM (10 years, 4 months ago)

Quote:

mushbecrazy said:
Sweet was hoping to turn this $30 pan cyan syringe into a infinite stock pile of mycelium. Lol  :shocked:



Knock up a few grain jars and a few plates.

G2G the jars out and spawn them.

At the same time clean up some cultures on agar.

Save the cultures for when you run out of grain spawn and need to knock up more jars.

Keep the culture alive forever in cold storage on slant tubes.

Keep going back to the agar cultures and tubes.

Never use the syringe again lol.


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Offlinerumfor69
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Re: agar culture from syringe? [Re: PussyFart]
    #18923523 - 10/02/13 09:30 PM (10 years, 4 months ago)

:whathesaid:

Use a nice chunk of cultured agar when inoculating a jar with agar.

use like a inchxinch square. Also dont shake the jar you put cultured agar

in. Let the agar sit on top the spawn in the jar and wait for it to spread

out some then shake the jar to fully spread around.


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: PussyFart]
    #18923536 - 10/02/13 09:32 PM (10 years, 4 months ago)

Whats a slant tube? Um sound like theres more then i was thinking involed.  My friend said had to use spore print, and something about puting them in a blown up ziplock bag


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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Offlinerumfor69
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923544 - 10/02/13 09:33 PM (10 years, 4 months ago)



--------------------

Links For Beginners And Beyond:mspoil:


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923545 - 10/02/13 09:33 PM (10 years, 4 months ago)

Oh can i use a pice of stem/cap off shroom.


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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Offlinerumfor69
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923552 - 10/02/13 09:36 PM (10 years, 4 months ago)

Use a baby pin that is growing in a sexy cluster.

Put the pin on agar and watch the fireworks it's called cloning


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: rumfor69]
    #18923576 - 10/02/13 09:42 PM (10 years, 4 months ago)

Lol would pins ever try to grow up on agar. Thatd be cool. Thank for the help.


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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Offlinerumfor69
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18923580 - 10/02/13 09:43 PM (10 years, 4 months ago)

Quote:

mushbecrazy said:
Lol would pins ever try to grow up on agar. Thatd be cool. Thank for the help.




I think they can but that wouldn't be a very good goal to achieve lol


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OfflineStromriderM
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Re: agar culture from syringe? [Re: rumfor69]
    #18923666 - 10/02/13 10:03 PM (10 years, 4 months ago)

Mushrooms can definitely grow on agar


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: Stromrider]
    #18924339 - 10/03/13 02:47 AM (10 years, 4 months ago)

What is sectors on mycelium. Im sure its easier to see in person and not on a iphone. But does " cleaning it up"  mean just to find the best mycelium. Idk Think im confusing my self


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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OfflineStromriderM
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18924352 - 10/03/13 02:52 AM (10 years, 4 months ago)

You take this one hacker I'm going night night :sleep:


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OfflinePussyFart
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Re: agar culture from syringe? [Re: mushbecrazy]
    #18924365 - 10/03/13 02:58 AM (10 years, 4 months ago)

Quote:

mushbecrazy said:
What is sectors on mycelium. Im sure its easier to see in person and not on a iphone. But does " cleaning it up"  mean just to find the best mycelium. Idk Think im confusing my self



http://www.mushroomvideos.com/Mushroom-Cloning

http://www.mushroomvideos.com/Strain-Isolation

Cleaning it up is referring to making sure it is a clean culture, free of contaminants....


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Offlinemushbecrazy
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Re: agar culture from syringe? [Re: PussyFart]
    #18924434 - 10/03/13 03:39 AM (10 years, 4 months ago)

Yep that cleared up a few things. Thanks been bunchesa ,of help


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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