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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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agar culture from syringe?
#18923421 - 10/02/13 09:11 PM (10 years, 4 months ago) |
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Can i start a agar petri with spore syringe? Pan cayan or P. Cubensis. If so how. Ive got 15 petri dishes ready for some thing.
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923429 - 10/02/13 09:12 PM (10 years, 4 months ago) |
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Yes.
Inoculate each dish with a drop or 2 of solution and let germinate and colonize.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,846
Loc: In the Gills
Last seen: 5 hours, 7 minutes
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Re: agar culture from syringe? [Re: PussyFart]
#18923443 - 10/02/13 09:14 PM (10 years, 4 months ago) |
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Yes, in fact you want to goto agar from spore.
it will give you the ability to grow a clean culture seems how spores are
never completely sterile. So you grow then clean first, cut and copy,
clean myce if the dish is contaminated on one side or something.
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: PussyFart]
#18923460 - 10/02/13 09:17 PM (10 years, 4 months ago) |
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Sweet was hoping to turn this $30 pan cyan syringe into a infinite stock pile of mycelium. Lol
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: rumfor69]
#18923488 - 10/02/13 09:23 PM (10 years, 4 months ago) |
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Quote:
rumfor69 said: Yes, in fact you want to goto agar from spore.
it will give you the ability to grow a clean culture seems how spores are
never completely sterile. So you grow then clean first, cut and copy,
clean myce if the dish is contaminated on one side or something.
Never thought about that. So i grow it then cut clean pieces out and start new ones. Or start all. from syringe. How long does a petri full last. How much do you throw in grain when done
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923498 - 10/02/13 09:25 PM (10 years, 4 months ago) |
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Quote:
mushbecrazy said: Sweet was hoping to turn this $30 pan cyan syringe into a infinite stock pile of mycelium. Lol 
Knock up a few grain jars and a few plates.
G2G the jars out and spawn them.
At the same time clean up some cultures on agar.
Save the cultures for when you run out of grain spawn and need to knock up more jars.
Keep the culture alive forever in cold storage on slant tubes.
Keep going back to the agar cultures and tubes.
Never use the syringe again lol.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,846
Loc: In the Gills
Last seen: 5 hours, 7 minutes
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Re: agar culture from syringe? [Re: PussyFart]
#18923523 - 10/02/13 09:30 PM (10 years, 4 months ago) |
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Use a nice chunk of cultured agar when inoculating a jar with agar.
use like a inchxinch square. Also dont shake the jar you put cultured agar
in. Let the agar sit on top the spawn in the jar and wait for it to spread
out some then shake the jar to fully spread around.
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: PussyFart]
#18923536 - 10/02/13 09:32 PM (10 years, 4 months ago) |
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Whats a slant tube? Um sound like theres more then i was thinking involed. My friend said had to use spore print, and something about puting them in a blown up ziplock bag
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,846
Loc: In the Gills
Last seen: 5 hours, 7 minutes
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923544 - 10/02/13 09:33 PM (10 years, 4 months ago) |
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923545 - 10/02/13 09:33 PM (10 years, 4 months ago) |
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Oh can i use a pice of stem/cap off shroom.
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,846
Loc: In the Gills
Last seen: 5 hours, 7 minutes
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923552 - 10/02/13 09:36 PM (10 years, 4 months ago) |
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Use a baby pin that is growing in a sexy cluster.
Put the pin on agar and watch the fireworks it's called cloning
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: rumfor69]
#18923576 - 10/02/13 09:42 PM (10 years, 4 months ago) |
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Lol would pins ever try to grow up on agar. Thatd be cool. Thank for the help.
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,846
Loc: In the Gills
Last seen: 5 hours, 7 minutes
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Re: agar culture from syringe? [Re: mushbecrazy]
#18923580 - 10/02/13 09:43 PM (10 years, 4 months ago) |
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Quote:
mushbecrazy said: Lol would pins ever try to grow up on agar. Thatd be cool. Thank for the help.
I think they can but that wouldn't be a very good goal to achieve lol
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 8 hours, 18 minutes
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Re: agar culture from syringe? [Re: rumfor69]
#18923666 - 10/02/13 10:03 PM (10 years, 4 months ago) |
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Mushrooms can definitely grow on agar
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: Stromrider]
#18924339 - 10/03/13 02:47 AM (10 years, 4 months ago) |
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What is sectors on mycelium. Im sure its easier to see in person and not on a iphone. But does " cleaning it up" mean just to find the best mycelium. Idk Think im confusing my self
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 8 hours, 18 minutes
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Re: agar culture from syringe? [Re: mushbecrazy]
#18924352 - 10/03/13 02:52 AM (10 years, 4 months ago) |
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You take this one hacker I'm going night night
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: agar culture from syringe? [Re: mushbecrazy]
#18924365 - 10/03/13 02:58 AM (10 years, 4 months ago) |
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Quote:
mushbecrazy said: What is sectors on mycelium. Im sure its easier to see in person and not on a iphone. But does " cleaning it up" mean just to find the best mycelium. Idk Think im confusing my self
http://www.mushroomvideos.com/Mushroom-Cloning
http://www.mushroomvideos.com/Strain-Isolation
Cleaning it up is referring to making sure it is a clean culture, free of contaminants....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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mushbecrazy
newbee


Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
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Re: agar culture from syringe? [Re: PussyFart]
#18924434 - 10/03/13 03:39 AM (10 years, 4 months ago) |
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Yep that cleared up a few things. Thanks been bunchesa ,of help
-------------------- I'm just some lunatic macaroni mushroom, is that it? -Joe Pesci
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