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Offlinesuniced
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Green mold contamination problem (pics)
    #18920708 - 10/02/13 11:54 AM (10 years, 3 months ago)

So i started my first mushroom grow recently. its been 8 days since inoculation and i already have jars with green mold. im using pf tek with 1/2 pint jars and have been storing them in a dark cabinet at 79°F. i flamed my needle until it was red, wiped down my glovebox with alcohol/lysol, used a tyvek suit, sterilized jars in a large pot for 80 minutes and let them cool overnight. anyways, 2/5 jars have shown contamination, 2 jars have shown mycelial growth, and one jar hasn't shown anything. Here are some pics-

Jar 1(mold) -

Jar 2(mycelium growth) -
                      -

Jar 3(no growth)-


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Offlineslap and tickle
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Re: Green mold contamination problem (pics) [Re: suniced]
    #18920746 - 10/02/13 12:05 PM (10 years, 3 months ago)

No need to keep the jars in the dark. Did you use a pressure cooker?  "In large pot"  Because just boiling wont get up to the temp you need.  15 psi is what your looking for. show a pic of your lid.  What are your injection ports like? A hole with tape, self sealing ect... Is there a filter for air?


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InvisibleB_BOY
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Re: Green mold contamination problem (pics) [Re: slap and tickle]
    #18920752 - 10/02/13 12:06 PM (10 years, 3 months ago)

steam sterilization is fine with brf jars


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InvisiblebodhisattaMDiscordReddit
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Re: Green mold contamination problem (pics) [Re: slap and tickle]
    #18920763 - 10/02/13 12:09 PM (10 years, 3 months ago)

Quote:

slap and tickle said:
No need to keep the jars in the dark. Did you use a pressure cooker?  "In large pot"  Because just boiling wont get up to the temp you need.  15 psi is what your looking for. show a pic of your lid.  What are your injection ports like? A hole with tape, self sealing ect... Is there a filter for air?




:smbfacepalm:

90m with a pot that has a tight fitting lid is sufficient for PF tek. The 1/2 pint PF jars only need to be at 212F for 90M in a steam bath. It's the only one that doesn't need a PC.

Where it may have went wrong:
If the lid wasn't tightly fitting you might have a problem.
If you flamed the needle long before you injected the jars there might be a problem.
Did you flame between every jar?
Did you start timing the steam sterilization after the boil was going for 5-10m and next time try for 90M or even 120M.
Also that jar that hasn't started growing yet isn't that old that it's crazy it hasn't started yet but please take the foil off of the top of it.




Edited by Trusted cuItivator (10/02/13 12:13 PM)


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Offlineslap and tickle
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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18920828 - 10/02/13 12:27 PM (10 years, 3 months ago)

I stand corrected sorry!! :grin:  But a PC is still better.quote]Damion5050 said:
The best 2 investments in this hobby are

1.  Pressure Cooker - Good one preferable

2.  Flow Hood






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OfflinePussyFart
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Re: Green mold contamination problem (pics) [Re: slap and tickle]
    #18920852 - 10/02/13 12:30 PM (10 years, 3 months ago)

Quote:

slap and tickle said:
I stand corrected sorry!! :grin:  But a PC is still better.



According to you..... but in reality steam sterilization works just fine for cakes, and a PC would not sterilize them any better.

You cannot kill anything deader than dead.


Edited by PussyFart (10/02/13 12:31 PM)


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Offlinempd
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Re: Green mold contamination problem (pics) [Re: slap and tickle]
    #18920858 - 10/02/13 12:32 PM (10 years, 3 months ago)

I don't know how anyone does cubes without a PC.  :shrug:


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OfflinePussyFart
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Re: Green mold contamination problem (pics) [Re: mpd]
    #18920860 - 10/02/13 12:33 PM (10 years, 3 months ago)

Quote:

mpd said:
I don't know how anyone does cubes without a PC.  :shrug:



www.mushroomvideos.com


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InvisiblebodhisattaMDiscordReddit
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Re: Green mold contamination problem (pics) [Re: slap and tickle]
    #18920899 - 10/02/13 12:41 PM (10 years, 3 months ago)

Quote:

slap and tickle said:
I stand corrected sorry!! :grin:  But a PC is still better.quote]Damion5050 said:
The best 2 investments in this hobby are

1.  Pressure Cooker - Good one preferable

2.  Flow Hood









Damion5050 is also the one that came up with the bucket tek which isn't proper pasteurization has led many to grow green but can be gotten away with using coir+verm+gypsum but you're playing roulette.

http://www.shroomery.org/forums/showflat.php/Number/11816158#11816158
Quote:

RogerRabbit said:
Quote:

bardleyrichard said:
And I thought I pasteurized the coir/coffee/verm mix, I have a 4 gallon bucket that I put the coir and stuff into, then I boiled 4.5 quarts of water, and let it cool for about 30 seconds and then threw it in there and covered with a cookie sheet with a towel on top:lol:




That is not a proper method of pasteurization.  The boiling water kills off many of the beneficial bacteria, leaving your substrate defenseless later. It can take up to an hour to kill mold spores, and you're unlikely to still have a proper temperature after that long unless you insulate the bucket, but then there's still the problem of the beneficial microbes being killed by the boiling water in the first few minutes. 

You should moisten the substrate to field capacity, and then gently heat to 140F to 160F, as measured at the center of the substrate with a meat thermometer and then maintain that temp for 1 to 1 1/2 hours.  You can see a short video clip of the process being done with casing material on my website in the casing prep tek.  Prepare your coir that way next time and you'll be more likely to get the mycelium to jump right off the grains and colonize the substrate before molds have a chance.
RR




http://www.shroomery.org/forums/showflat.php/Number/14791499#14791499
Quote:

RogerRabbit said:
Quote:

OregonChronic said:
I put 1 brick of coir and 2qts of verm(fine verm sand-like) in a 5gal bucket and added 4qts of boiling hot water in the bucket and sealed the lid for 45mins




That is neither suitable for hydration or pasteurization.  You're supposed to fully hydrate the coir to field capacity, and then slowly heat it to 140F/60C and hold it there for 60 to
90 minutes.  Pouring boiling water on dry coir accomplishes none of the above.  Use proper technique if you wish to achieve success.

Also, hydrate the verm and coir or other bulk substrate ingredients separately and then mix later before pasteurization.
RR




http://www.shroomery.org/forums/showflat.php/Number/18764803#18764803
Quote:

FrankHorrigan said:
Oh for god's sake, back then they didn't know as definitively as they do now that it can fail for no other reason than the bucket tek. There is a reason that people push proper pasteurization.


You
speak like someone who hasn't had enough green in his shit to know what he's talking about, so you should back off respectable members that you know little about.

On the other hand, I have personally run hundreds of tubs at this point. I can say without a doubt that bucket tek is unreliable, esp after you've had some contam issues in your grow area.

If you want your best chance of success, properly pasteurize. if you want to chance it, go for it, bucket tek works for a lot of people.

But don't waste everyone's time saying that the bucket tek can't cause issues for multiple tubs out of ten, I've seen it happen more than once.

Quote:

Jive turkey said:
had all my monotubs contaminate and I can figure out where I goofed up.
my jars were 100% colonized with no signs of contams and my substrate was pasteurized some tubs done with bucket tek others done on stovetop with candy thermometer.

the all contaminated soon after the holes were stuffed




I bet your culture or spore syringe was contam'd.

100% failure rate is usually a sign of something going wrong initially, esp since you properly pasteurized.




http://www.shroomery.org/forums/showflat.php/Number/18237521#18237521
Quote:

FrankHorrigan said:
Quote:

xaylon420 said:
bucket tek works fine with non bricked coir. honestly, bucket tek WORKS period as long as you follow it to the T lol. i don't understand how so many ppl mess it up....was your buckets, oust the air.....never failed me :laugh:




The bucket tek fails, it's not always the people who mess it up. Bucket tek is not proper pasteurization. Especially with loose coir which will absorb heat more readily, you're running a higher than average risk of partial sterilization vs a proper pasteurization method.

To everyone- non-brick coir is expensive as hell. I used to expand my coir bricks and let it dry before putting it away. Instant loose coir :wink:






Edited by Trusted cuItivator (10/02/13 12:44 PM)


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OfflineNakor420
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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921121 - 10/02/13 01:15 PM (10 years, 3 months ago)

TOSS IT!


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InvisiblebodhisattaMDiscordReddit
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Re: Green mold contamination problem (pics) [Re: Nakor420]
    #18921132 - 10/02/13 01:17 PM (10 years, 3 months ago)

I'm sure he is planning on birthing it and putting it in his FC...


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OfflineNakor420
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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921272 - 10/02/13 01:18 PM (10 years, 3 months ago)

Quote:

bodhisatta said:
I'm sure he is planning on birthing it and putting it in his FC...





Yeah I figured, that's why I said that. :facepalm3:


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Offlinesuniced
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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921274 - 10/02/13 01:18 PM (10 years, 3 months ago)

Thank you all for your responses! really appreciate it.

1- picture of the pot- [image][/image]
[image][/image]

2- i did flame the needle for about 20 seconds
3- i flamed between every jar
4- i started the timer after it was boiling
5- haha i will take the foil off


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InvisiblebodhisattaMDiscordReddit
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Re: Green mold contamination problem (pics) [Re: suniced]
    #18921332 - 10/02/13 01:29 PM (10 years, 3 months ago)

Yea, I guess I can't see where you could have went wrong unless it was the syringe but it should contaminate every jar then.


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Offlinesuniced
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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921441 - 10/02/13 01:50 PM (10 years, 3 months ago)

the only thing i can think of is that my substrate was too moist, but im a noob at this haha. thanks for your input!


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Its not the years in your life that count
                       
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InvisiblebodhisattaMDiscordReddit
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Re: Green mold contamination problem (pics) [Re: suniced]
    #18921511 - 10/02/13 02:06 PM (10 years, 3 months ago)

2 parts verm 1 part brf 1 part water (not distilled) ? mix verm and water first then add brf and loose fill the jars. verm barrier on top but not compacted. wipe the top part of the jar before the verm barrier went on too?


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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921521 - 10/02/13 02:09 PM (10 years, 3 months ago)

Quote:

bodhisatta said:
2 parts verm 1 part brf 1 part water (not distilled) ?



Never use distilled water.....use tap water, pond water, rain water, toilet water, but not distilled water.


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Re: Green mold contamination problem (pics) [Re: PussyFart]
    #18921542 - 10/02/13 02:14 PM (10 years, 3 months ago)

I did mean that as in yes don't use distilled...


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Re: Green mold contamination problem (pics) [Re: bodhisatta]
    #18921553 - 10/02/13 02:16 PM (10 years, 3 months ago)

:thumbup::cool:


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