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Offlinehihihi1717
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First time growing, mycelium or contamination?
    #18918111 - 10/01/13 08:13 PM (10 years, 3 months ago)









These are 3 of the jars we inoculated and I'm wondering if they look "healthy"? Looks like the beginning of mycelium growth but wouldn't mind a second opinion on the matter being its our first grow. Thanks again.


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OfflineJimmyhunter1000
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18919628 - 10/02/13 05:05 AM (10 years, 3 months ago)

Definitely Contam. I'm not sure if its black mold or bacteria, but either way that's certainly no good.


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: Jimmyhunter1000]
    #18920104 - 10/02/13 09:19 AM (10 years, 3 months ago)

Quote:

Jimmyhunter1000 said:
Definitely Contam. I'm not sure if its black mold or bacteria, but either way that's certainly no good.




All three jars? And is there any mycelial growth at all from your view? The reason I ask is we fucked the grow up pretty bad and it would be miraculous if we even got mycelial growth despite the contamination (this means we're at least keeping them in a decent environment, despite the lack of sterilization when it mattered).


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OfflineRauhfasertapete
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18920123 - 10/02/13 09:26 AM (10 years, 3 months ago)

I cant see anything that looks like a mycelium, and I guess not even mold would grow in there because the jars are quite a bit under water. Especially in the lower one you can see some kind of white soup, the colour probably comes from starch particles flowing in there.

Throw them away and try it again with a lower water content. its actually better when its a bit too dry than when its too moist.


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OfflineRauhfasertapete
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18920135 - 10/02/13 09:32 AM (10 years, 3 months ago)

I´d recommend the standard beginners recipe:

2 parts by volume vermiculite
1 part ground rice / rice flour
1 part water

or

soak your verm in water, allow the water to drip off, and mix it with dry rice flour, which will then stick to the surface of the vermiculite pieces


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: Rauhfasertapete]
    #18920929 - 10/02/13 12:47 PM (10 years, 3 months ago)

Yeah, I found out that besides my horribly nonsterile procedure, I had modified the ratio of verm:water:brf because I didn't mix the water with the verm first, which caused the substrate to look way too dry. Had I known about the order of operations in mixing, and the necessity of the 2:1:1 ratio, this would have been avoided. Was just wondering if there was anything worth noting here in terms of growth, despite what I suppose is now obvious contamination. Thanks for the responses, everyone.


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OfflineKizzle
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18923337 - 10/02/13 08:48 PM (10 years, 3 months ago)

It looks like there may have been mycelium of some kind but definitely contaminated with bacteria.


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: Kizzle]
    #18924303 - 10/03/13 02:30 AM (10 years, 3 months ago)

Quote:

Kizzle said:
It looks like there may have been mycelium of some kind but definitely contaminated with bacteria.




I'm starting to wonder if the substrate was so wet (and not sterile) that A) it became a cesspool for bacteria and molds, and B) caused the spores at the inoculation point to spread out EVERYWHERE and lead to an extremely stunted growth rate, as they are competing and fighting with bacteria and mold for nutrients that are already scarce due to the extra water. Hopefully this means if I do my substrate correctly and use more sterile environments that colonization will occur sooner and show healthy, uncontaminated mycelial growth. A man can hope, anyway.


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OfflineKizzle
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18924403 - 10/03/13 03:21 AM (10 years, 3 months ago)

If they were anywhere near as wet as they look now when you first took them out of the PC or pot then you probably got water in them during sterilization. Which would also mean the dry verm layer got wet allowing bacteria through.


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: Kizzle]
    #18927571 - 10/03/13 07:27 PM (10 years, 3 months ago)

Definitely, I didn't know to use micropore/medical tape along with the tin foil, so I'm sure that definitely fucked them up.


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OfflineKizzle
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18929636 - 10/04/13 04:15 AM (10 years, 3 months ago)

If you had foil over them I'm guessing you didn't have them elevated out of the water during sterilization. That's usually the reason water gets in.


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: Kizzle]
    #18930010 - 10/04/13 07:48 AM (10 years, 3 months ago)

We filled the PC with water up to the bottom of the neck of the bottle, so there was probably a 1/2 inch gap between the surface level of the water and the rim of the jar. The tin foil would be partially in the water and the rest on top of the jar, but without the micropore tape, we steamed some of the contents into the PC, and likewise, got our substrate even more wet than it already was (I fucked up the verm:water:brf ratio by adding another cup of water because it looked too dry).


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Invisible8ow8
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18930379 - 10/04/13 09:41 AM (10 years, 3 months ago)

Check your PC's instructions - most (All Americans included) Require only 1.5 inches of water to operate. Steam is all you need to sterilize!

See here, page 18: http://fantes.com/manuals/all-american-pressure-cooker-manual.pdf


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OfflineKizzle
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Re: First time growing, mycelium or contamination? [Re: 8ow8]
    #18930987 - 10/04/13 12:21 PM (10 years, 3 months ago)

You want them so the entire jar is out of the water not just the rim. There's a lot of water splashing around from the boiling, and can actually reach pretty high and get through under the foil.


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Offlinehihihi1717
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Re: First time growing, mycelium or contamination? [Re: 8ow8]
    #18931023 - 10/04/13 12:30 PM (10 years, 3 months ago)

This PDF says that 10 psi, which is about what my PC can put out (11.6 psi to be exact) will only reach a temperature of 240 degrees Fahrenheit. Will this be sufficient to sterilize the contents of my brf jars? If it's a bit low (I see 250 degrees Fahrenheit as recommended), will cooking for extra time compensate?


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OfflineRauhfasertapete
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Re: First time growing, mycelium or contamination? [Re: hihihi1717]
    #18931097 - 10/04/13 12:49 PM (10 years, 3 months ago)

240 should still be nough for our purposes. anyway, sterility is not all, your problem may have been moisture, my problem sometimes was oxygen supply for the growing mycelium... there are some parameters that are more important than absolute sterility, anyway your inoculate probably isn´t 100% sterile.


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