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Offlinewestthebest
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spawn bag inoculation w/o flow hood?
    #18916080 - 10/01/13 12:54 PM (10 years, 3 months ago)

trying out a 3lb pre-sterilized bag of rye with an injection port, what is the best way to do this without a flowhood?  the over door tek is bullshit if I'm not mistaken, are you supposed to just find a clean room with no air flow and go for it, or do you need to build a big enough glove box to fit the bags in?  that would be a bummer because I like mine but don't think its big enough.  thanks guys.


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InvisiblebodhisattaMDiscordReddit
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18916091 - 10/01/13 12:56 PM (10 years, 3 months ago)

Flame needle and put through the injection port. Wipe injection port with alcohol first. The injection port is going to be just as dirty in front of a flow hood, in a SAB, or on your kitchen table.

A flow hood, SAB, or glovebox would be handy if you were wanting to put a agar wedge into the bag or grain to grain transfer into or out of that bag. It's pretty unnecessary if you are using a SHIP though but if you had one you could for the extra precaution.


Edited by Trusted cuItivator (10/01/13 12:59 PM)


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OfflineJamesSpawned
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18916105 - 10/01/13 12:59 PM (10 years, 3 months ago)

I've heard of people having success using a garbage bag but I don't think that it's too great an idea. I would seriously consider building a larger SAB if I were you or at least doing multible runs in your small SAB.


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OfflineJamesSpawned
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Re: spawn bag inoculation w/o flow hood? [Re: JamesSpawned]
    #18916114 - 10/01/13 01:00 PM (10 years, 3 months ago)

Sorry, I misread. You have just one bag and your SAB is not large enough to place it in?


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: JamesSpawned]
    #18916198 - 10/01/13 01:16 PM (10 years, 3 months ago)

Quote:

JamesSpawned said:
Sorry, I misread. You have just one bag and your SAB is not large enough to place it in?



I made it with the bare minimum size for pf tek jars in mind haha.  and actually I have multiple bags...  so, glovebox necessary or no?  seems that some people really think it isn't but then again I don't know why it would be any less necessary than with the pf tek...?


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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18916241 - 10/01/13 01:27 PM (10 years, 3 months ago)

Even with a PF tek you can inoculate on your counter. You flame the needle outside of your SAB anyway. The hot needle takes care of the contams on the skin of the injection port and there's even less of them since you wipe it with alcohol. Now we all know alcohol only sanitizes the patch is just as dirty no matter what other sterile precautions you take. I mean I wouldn't do it open air with a fan on and I'll use a SAB just to be overkill sometimes, but I have never had a contamination attributed to injection in open air that couldn't be attributed to a failure somewhere else.


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: bodhisatta]
    #18916308 - 10/01/13 01:41 PM (10 years, 3 months ago)

another question: I read somewhere that you have to go through 5 days of repeatedly pulling the sides of the bags apart before you inoculate to give the mycelium enough air to grow, is this true?


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OfflineJamesSpawned
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18916571 - 10/01/13 02:45 PM (10 years, 3 months ago)

Many people inoculate outside of a sab with no problems as stated above by bodhisatta, doing it in a SAB is obviously more ideal though. As for pulling on the bag to provide air: most often all that is necessary is to hang the bag up and let gravity pull the air through the filter patch.


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: JamesSpawned]
    #18917225 - 10/01/13 04:52 PM (10 years, 3 months ago)

Quote:

JamesSpawned said:
Many people inoculate outside of a sab with no problems as stated above by bodhisatta, doing it in a SAB is obviously more ideal though. As for pulling on the bag to provide air: most often all that is necessary is to hang the bag up and let gravity pull the air through the filter patch.



so I should do that when I receive the bags, before inoculating, or can I inoculate then hang them?  is it necessary?


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InvisibleFrankHorrigan
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18917775 - 10/01/13 06:59 PM (10 years, 3 months ago)

Don't work with your cultures in open air. They are most susceptible to contamination when you are working with sterile media.

Sure, you can get away with it. Does this mean you should go for it? Hell no.

Get a bigger SAB :wink:


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: FrankHorrigan]
    #18918160 - 10/01/13 08:26 PM (10 years, 3 months ago)

Quote:

FrankHorrigan said:
Don't work with your cultures in open air. They are most susceptible to contamination when you are working with sterile media.

Sure, you can get away with it. Does this mean you should go for it? Hell no.

Get a bigger SAB :wink:



ah, just the man I wanted to see.  made a bigger one earlier this afternoon, gonna have to go and get some clamps to hang the bags up though.


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18919224 - 10/02/13 12:34 AM (10 years, 3 months ago)

anybody not hang their bags?  I believe the instructions provided say just set them down to let them colonize but...?


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Offlinemagicbroncoride
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18919263 - 10/02/13 12:42 AM (10 years, 3 months ago)

Mine are sitting on a shelf below my tv that way I can see if I hit the multi spore loto. I have never heard of them being suspended. Except for the couple I watch "race" the others be they bags or jars or whatever are on a shelf ignored. Seems to work pretty well.


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InvisibleFrankHorrigan
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Re: spawn bag inoculation w/o flow hood? [Re: magicbroncoride]
    #18920214 - 10/02/13 10:01 AM (10 years, 3 months ago)

There is no reason to ever hang a bag :shrug:

They colonize fine wherever you set them.


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: FrankHorrigan]
    #18924244 - 10/03/13 01:44 AM (10 years, 3 months ago)

huh, I just heard it was because you had to get air in there mycelium for it grow...?  good to hear though.  thanks.


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InvisibleOeric McKenna
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Re: spawn bag inoculation w/o flow hood? [Re: westthebest]
    #18924308 - 10/03/13 02:32 AM (10 years, 3 months ago)

Are you working witgh agar or needles??
That's the key question.
  You can inject a red hot needle just fine & the first drops will self cool it.

On the "air to the mycelium" idea, its more of a c02 away from the mycelium idea, and that's what the filter patch is for.


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Offlinewestthebest
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Re: spawn bag inoculation w/o flow hood? [Re: Oeric McKenna]
    #18924463 - 10/03/13 04:05 AM (10 years, 3 months ago)

Quote:

Oeric McKenna said:
Are you working witgh agar or needles??
That's the key question.
  You can inject a red hot needle just fine & the first drops will self cool it.

On the "air to the mycelium" idea, its more of a c02 away from the mycelium idea, and that's what the filter patch is for.



definitely what I thought, for some reason I've seen some people do it even with a filter patch though.

oh, and needles.  good to know.


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