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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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dont know what to do, lost, confused, help!! pics attached..
#18915458 - 10/01/13 10:29 AM (10 years, 3 months ago) |
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Really confused and lost in this grow. I've had many successful but im not sure what went wrong. So I started over with 200 jars. Spores purchased from trusted supplier. Pc'd at an 1:30 at 15psi. Saw growth on almost all of them after on 3 days. Good amounts of growth actually... but the mycelium seemed thin, but was colonizing fast. But I also had some jars with thicker mycelium that looked right too me imo. I lost my usual 10-20% in contaminations from using a still air box. I question the mycelium in an earlier post, bc it just didnt seem right too me...,(http://www.shroomery.org/forums/showflat.php/Number/18852889#18852889) I finally spawned to bulk sub, the jars with the thicker myc colonized well... but every tub made with the thinner stuff i can see in and the grains just seem to fuzz up but not colonize, i included a picture of 1 i did 9 days ago. It should def be colonized after 10 days right? Do you think this could be a problem with the original syringe because i cant see myself contaminating 140 jars give or take including the ones i can see the contamination in. I attached pictures... im freakin out kinda. Put a lot of hard work into this... deciding wether to try the rest of this spawn or not...





-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: HiLIFE]
#18915553 - 10/01/13 10:51 AM (10 years, 3 months ago) |
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Quote:
I lost my usual 10-20% in contaminations from using a still air box.
wow that's a huge amount of jars to toss. I don't even use a still air box to inoculate through my SHIPs and I have never got a rate that terrible. You shouldn't even get 10=20% contams doing g2g transfers.
Take a better picture of the top of one of your jars. Is that just a piece of tyvec, and if so how is it attached? If that's micropore tape that you're inoculating through that's your problem.
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: bodhisatta]
#18915585 - 10/01/13 10:58 AM (10 years, 3 months ago) |
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These were all inoculated using 20 syringes! I use two pieces of micropore tape for the filters, when I inoculate i just open the cap slightly and squirt a cc in. 10-20% has always been a normal rate for me. Maybe its where I live. And whats a SHIP?
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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Dark76


Registered: 07/14/13
Posts: 121
Last seen: 5 years, 8 months
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: HiLIFE]
#18915605 - 10/01/13 11:04 AM (10 years, 3 months ago) |
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SHIP = Self Healing Injection Port
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: HiLIFE]
#18915608 - 10/01/13 11:04 AM (10 years, 3 months ago) |
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Quote:
HiLIFE said: These were all inoculated using 20 syringes! I use two pieces of micropore tape for the filters, when I inoculate i just open the cap slightly and squirt a cc in. 10-20% has always been a normal rate for me. Maybe its where I live. And whats a SHIP?
There's your problem. Opening the cap is causing you to lose 10-20% right there plus micropore tape sucks except as extra protection for a PF jar that has a verm barrier already. Besides that I'm sure some of the jars you think are successful actually have some bacteria in them too.
SHIP=self healing injection port. IE a 1/4-3/8" hole that is filled with RTV or 100% silicone. Flame needle and push through the silicone port which heals itself when you remove the needle. What also works is the same hole stuffed extremely tight with polyfil, you can inject through that too and both of these ways will give you WAY more success than the way you're inoculating.
Look up some lid teks.
Edited by Trusted cuItivator (10/01/13 11:07 AM)
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: bodhisatta]
#18915624 - 10/01/13 11:08 AM (10 years, 3 months ago) |
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so wait, you dont use a still air box at all, just a hole filled with rtv or silicone, heat, stick, squirt, repeat?
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: bodhisatta]
#18915655 - 10/01/13 11:15 AM (10 years, 3 months ago) |
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That's exactly what I'm saying. Why would it be any more helpful to use a SAB the needle is flamed outside of the SAB any way. The port is cleaned with alcohol any way (just as dirty inside or outside the SAB).
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: bodhisatta]
#18915706 - 10/01/13 11:26 AM (10 years, 3 months ago) |
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even so, ive still never lost this many jars before, this is just crazy. really upset its not working. how does rtv do pressure cooking, thats good info for my future endeavors, but does not help me make my decision on what to do now. toss tubs and jars, or use them, or just wait and see.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: dont know what to do, lost, confused, help!! pics attached.. [Re: HiLIFE]
#18916004 - 10/01/13 12:40 PM (10 years, 3 months ago) |
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RTV is the material they sometimes use to make gaskets on engines etc... The pressure cooker actually will cure it before the curing time if it were left out.(if it's starting to form a skin you can put it in the PC even if it's soft)
Wait and see with the jars. When you open them(after they're 100% colonized and shook) if they smell like anything other than fresh mushrooms (including fresh mushrooms + smell of something bad) then toss them out.
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