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Offlinepurplepixie
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Registered: 08/20/13
Posts: 64
Loc: Ireland (W)
Last seen: 3 years, 9 days
Lab Air Filteration ideas welcome..
    #18911662 - 09/30/13 02:58 PM (10 years, 3 months ago)

Hey all,

I'm a complete newb to cultivation and am digesting Stamet's with great enthusiasm! I can't wait to grow mushrooms...i live in a damp place here in ireland...well the whole country is damp and dark!...so i have plenty of places in woodlands outside to do some outdoor beds and stumps to inoculate and some cool dark barns to grow indoors too.

I'm just wondering what you guys use for air filtering your lab environment to make your own cultures.
Is it possible to build your own?
I'm thinking of using a very small room i have as my 'clean room'...and i'm handy with tools and building stuff so don't mind building a clean box...i'm trying to cut costs down so if you know of any places (im in Ireland so Europe suppliers would be more handy)who supply the raw filters for a good deal that would be really useful.

Any ideas you can give me for creating cheap and effective air filteration would be great before i embark on this and do it all wrong!

Thanks in advance for any advice :-)


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SEEK, AND YE SHALL FIND...


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Invisiblesolarity
mm... my favourite food
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Registered: 03/31/09
Posts: 1,590
Loc: UK Flag
Trusted Cultivator
Re: Lab Air Filteration ideas welcome.. [Re: purplepixie]
    #18912288 - 09/30/13 05:44 PM (10 years, 3 months ago)

You keep the room clean with very fine but not usually HEPA (I use a face mask and a vent fan) filter on it to keep +ve pressure constantly.
Then you do all your work in front of a Laminar Flow Hood. Search on here for LFH, Hood or flow hood there are lots of building instructions. From the functional/ugly to anything made by Stonesun!
HEPA filters come up on Ebay occasionally.


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Commercial exotics farmer for 8 years - now sold up!


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InvisibleAleon
The Power of Our Origins
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Registered: 05/26/11
Posts: 1,127
Loc: Everywhere
Re: Lab Air Filteration ideas welcome.. [Re: solarity]
    #18914963 - 10/01/13 08:00 AM (10 years, 3 months ago)

Get a good flow hood and youll be fine.  If you want to go for the gusto add positive pressurization to the lab using filters (i use a pre-filter + Laminar grade HEPA)


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OfflineTaoShroom
Novice Mycologist
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Registered: 03/08/12
Posts: 135
Loc: Canada
Last seen: 5 years, 3 months
Re: Lab Air Filteration ideas welcome.. [Re: Aleon]
    #18915533 - 10/01/13 10:46 AM (10 years, 3 months ago)

A flow hood is only really needed if you plan on doing a lot of open air transfers of grain/plugs/agars and what not. For indoor grows you'll want to construct a grow room, which would be clean, have FAE (filtered air exchange), as well as high RH (relative humidity). You can construct rooms out of PVC and plastic sheeting, or just use an open space room of concrete, almost anything will work. Just keep the room as clean as possible, and don't be afraid to pull everything out between flushes and give it a go over.
That being said, I like your ideas for inoculating outdoor stumps and what not. Get a Pressure Cooker, learn how to make spawn and plugs. You don't need to filter the air in your room to make spawn jars. Find a TEK here on the Shroomery for making lids for your jars, they will do the air filtration for you, so long as you sterilize the contents b4 inoculating them with spores/LC. Here is a decent TEK here for spawn lids.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8364022


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Curved like a strait line.


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OfflineTaoShroom
Novice Mycologist
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Registered: 03/08/12
Posts: 135
Loc: Canada
Last seen: 5 years, 3 months
Re: Lab Air Filteration ideas welcome.. [Re: TaoShroom]
    #18915543 - 10/01/13 10:49 AM (10 years, 3 months ago)

Also if you do plan on transferring agar slants to isolate cultures, start with a SAB (still air box).
http://www.shroomery.org/forums/showflat.php/Number/17818078#17818078


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Strait like a jagged arrow.
Curved like a strait line.


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Offlinepurplepixie
ForestLurker
Female User Gallery

Registered: 08/20/13
Posts: 64
Loc: Ireland (W)
Last seen: 3 years, 9 days
Re: Lab Air Filteration ideas welcome.. [Re: TaoShroom]
    #18922072 - 10/02/13 03:50 PM (10 years, 3 months ago)

Thanks so much for the ideas guys...it's quite a lot to take in and consider...i really want to be making my own spawn rather than buying it in , for sure!

I am thinking i will start outdoor beds with my own spawn...then once i get confident will set up an indoor room.

It's going to be amazing to grow mushrooms :-)


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SEEK, AND YE SHALL FIND...


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Invisibleliamtheloser
Advanced Idiot
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Registered: 06/07/06
Posts: 1,453
Re: Lab Air Filteration ideas welcome.. [Re: purplepixie]
    #18922894 - 10/02/13 06:50 PM (10 years, 3 months ago)

It's very simple to prepare and inoculate your own spawn. I even work with agar using a "still air box", and fingers crossed,  haven't had a contamination issue yet.  My still air box is a small bathroom with a large tote turned on its side.  I use a surgeons mask and gloves to minimize accidental transfer,  but you definitely don't need a room with filtered air by any means.


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