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OfflineJahLambsbread
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Can you clone without Agar?
    #18911436 - 09/30/13 02:02 PM (10 years, 3 months ago)

Can you clone tissue from the cap without using agar to grow mycelium in?


Someone said you can just put it in a plastic bag and it will grow fine in that.
Will that work?


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: JahLambsbread]
    #18911454 - 09/30/13 02:06 PM (10 years, 3 months ago)

I have seen tissue added to grain jars and that started colonizing. I never saw the end result though.


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OfflineOregonMushys
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Re: Can you clone without Agar? [Re: JahLambsbread]
    #18911500 - 09/30/13 02:18 PM (10 years, 3 months ago)

There is ghetto brf agar recipes you can do in half pint jars lol. Though i would use a pressure cooker and make up some agar from one of the recipes on here, and pour some disposable petris.

http://www.shroomery.org/forums/showflat.php/Number/14546083#14546083


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OfflinecronicrFacebook
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911506 - 09/30/13 02:21 PM (10 years, 3 months ago)

Quote:

Sagescruffy said:
I have seen tissue added to grain jars and that started colonizing. I never saw the end result though.



the results are not worth the effort, but it's easy enough to use flour and water in a half pint


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: cronicr]
    #18911526 - 09/30/13 02:25 PM (10 years, 3 months ago)

Quote:

cronicr said:
Quote:

Sagescruffy said:
I have seen tissue added to grain jars and that started colonizing. I never saw the end result though.



the results are not worth the effort, but it's easy enough to use flour and water in a half pint




Did you see the experiment I'm talking about? The guy took a fresh fruit, made a biopsy of the interior tissue from the mushroom and dropped that tissue into a quart jar of sterilized wbs. This was done inside a still air box and the tissue actually started to grow out and colonize the wbs. I don't know what happened to that thread or what results the guy got.


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911543 - 09/30/13 02:30 PM (10 years, 3 months ago)

Notahacker420 and I have both successfully taken tissue to grain but it's a crap shoot. I would recommend doing several jars because some will contam. Actually I would just recommend agar. It is really fun to work with anyway


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: Stromrider]
    #18911570 - 09/30/13 02:35 PM (10 years, 3 months ago)

What was the success rate?


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911597 - 09/30/13 02:40 PM (10 years, 3 months ago)

If I remember correctly hacker did 10 jars and got one good one. I did one jar and somehow got lucky :shrug: I probably couldn't do it again if I tried.


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: Stromrider]
    #18911633 - 09/30/13 02:48 PM (10 years, 3 months ago)

How did you do it exactly? I think it definitely makes a difference where the sample came from on or in the mushroom. Thank you for the input btw, I have been curious about the results of that thread since I saw it.


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Love.


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InvisibleZombi3
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911660 - 09/30/13 02:57 PM (10 years, 3 months ago)

I took a tissue sample from the center of a stem and used it to inoculate a liquid culture. Is this not a clone? Like if I suck up 10 syringes out of it are they not all the same clone culture?


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: Zombi3]
    #18911663 - 09/30/13 02:58 PM (10 years, 3 months ago)

Did you succeed with that?


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911671 - 09/30/13 03:01 PM (10 years, 3 months ago)

I just took a piece from the center of the stipe and dropped it in. I did it in a sab


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: Stromrider]
    #18911701 - 09/30/13 03:10 PM (10 years, 3 months ago)

What did you do with the jar that you transferred a tissue sample to?


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18911855 - 09/30/13 03:49 PM (10 years, 3 months ago)

When it fully colonized I g2g it to 7 more jars and made a monotub. :cool:


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OfflineSagescruffy
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Re: Can you clone without Agar? [Re: Stromrider]
    #18911868 - 09/30/13 03:52 PM (10 years, 3 months ago)

Ah shit :headbanger:


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Offlinenn-IlliniSpiralDMT
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Re: Can you clone without Agar? [Re: Sagescruffy]
    #18912157 - 09/30/13 05:14 PM (10 years, 3 months ago)

Just buy agar and 10 petri dishes, it will cost 10$ tops. And it is so worth it, cloning on agar is fun. Seeing the mycelium spawn out is beautiful:mushroom2:


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: nn-IlliniSpiralDMT]
    #18912950 - 09/30/13 08:02 PM (10 years, 3 months ago)

Quote:

nn-IlliniSpiralDMT said:
Just buy agar and 10 petri dishes, it will cost 10$ tops. And it is so worth it, cloning on agar is fun. Seeing the mycelium spawn out is beautiful:mushroom2:




:whathesaid:


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OfflineTheApprentice
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Re: Can you clone without Agar? [Re: Stromrider]
    #18913138 - 09/30/13 08:52 PM (10 years, 3 months ago)

:jacked:

A QUESTION.

I have a big mushroom I want to clone, but can't get around to it until tomorrow.  I picked it and put it in bag in my fridge.

Will it still be ok to clone tomorrow or the day after?

:jacked:


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OfflineStromriderM
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Re: Can you clone without Agar? [Re: TheApprentice]
    #18913330 - 09/30/13 09:34 PM (10 years, 3 months ago)

Yeah it should be fine


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Offlinespoonfed
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Re: Can you clone without Agar? [Re: Stromrider]
    #18913804 - 09/30/13 11:07 PM (10 years, 3 months ago)



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Invisibleazur
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Re: Can you clone without Agar? [Re: spoonfed]
    #18913838 - 09/30/13 11:16 PM (10 years, 3 months ago)

When I've done it in the past, I put the inner piece of mushroom in a Ziploc bag for a few days until it gets fuzzy.  The I use it to make a liquid culture.
Success rate was probably 80%. 
I don't know that I would call it cloning per say, though you will see better results than multi spore.


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OfflineTheApprentice
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Re: Can you clone without Agar? [Re: spoonfed]
    #18914006 - 10/01/13 12:11 AM (10 years, 3 months ago)

Quote:

spoonfed said:
here bro: http://www.shroomery.org/8514/Agar-substitute





OP,

I have used this tek with much success.  The only thing is, the BRF paste is a little harder to cut and make transfers of than agar.

Order my agar online and use these jars

That way I pour my agar then pc it. 

don't have the extra step of pouring before the pc for exposure to contams.  #simple


--------------------


RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek Easy COIR Trays! *
Pink Oysters on Newspaper TEK

"Yeah?  Well, DRACULA called... and he said he's coming over tonight, and I said OK!" :mastershake:


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Offlineaussieshroomer1
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Re: Can you clone without Agar? [Re: TheApprentice]
    #18914251 - 10/01/13 01:33 AM (10 years, 3 months ago)

would making a lc from a piece of tissue yield good results


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Offlineaussieshroomer1
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Re: Can you clone without Agar? [Re: aussieshroomer1]
    #18914258 - 10/01/13 01:35 AM (10 years, 3 months ago)

sorry to hijack just wondering


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InvisibleOgla
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Re: Can you clone without Agar? [Re: aussieshroomer1]
    #18914277 - 10/01/13 01:40 AM (10 years, 3 months ago)

ive seen people use cardboard to clone.  never done it, but worked in the threads posted


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OfflineSupaThaRipper
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Re: Can you clone without Agar? [Re: Ogla]
    #18914364 - 10/01/13 02:06 AM (10 years, 3 months ago)

So many ways to clone. Agar, lc, grain, cardboard. But agar is by far the best.


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