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Invisibleazur
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Registered: 04/21/12
Posts: 28,103
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Re: Can you clone without Agar? [Re: spoonfed]
    #18913838 - 09/30/13 11:16 PM (10 years, 3 months ago)

When I've done it in the past, I put the inner piece of mushroom in a Ziploc bag for a few days until it gets fuzzy.  The I use it to make a liquid culture.
Success rate was probably 80%. 
I don't know that I would call it cloning per say, though you will see better results than multi spore.


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OfflineTheApprentice
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Registered: 09/25/11
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Re: Can you clone without Agar? [Re: spoonfed]
    #18914006 - 10/01/13 12:11 AM (10 years, 3 months ago)

Quote:

spoonfed said:
here bro: http://www.shroomery.org/8514/Agar-substitute





OP,

I have used this tek with much success.  The only thing is, the BRF paste is a little harder to cut and make transfers of than agar.

Order my agar online and use these jars

That way I pour my agar then pc it. 

don't have the extra step of pouring before the pc for exposure to contams.  #simple


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Offlineaussieshroomer1
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Registered: 12/02/12
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Re: Can you clone without Agar? [Re: TheApprentice]
    #18914251 - 10/01/13 01:33 AM (10 years, 3 months ago)

would making a lc from a piece of tissue yield good results


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Offlineaussieshroomer1
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Registered: 12/02/12
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Re: Can you clone without Agar? [Re: aussieshroomer1]
    #18914258 - 10/01/13 01:35 AM (10 years, 3 months ago)

sorry to hijack just wondering


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InvisibleOgla
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Registered: 02/16/04
Posts: 11,314
Re: Can you clone without Agar? [Re: aussieshroomer1]
    #18914277 - 10/01/13 01:40 AM (10 years, 3 months ago)

ive seen people use cardboard to clone.  never done it, but worked in the threads posted


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OnlineSupaThaRipper
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Registered: 09/02/13
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Re: Can you clone without Agar? [Re: Ogla]
    #18914364 - 10/01/13 02:06 AM (10 years, 3 months ago)

So many ways to clone. Agar, lc, grain, cardboard. But agar is by far the best.


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