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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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yeah, thats what i thought too that if there was a contam atleast THAT would show some growth. nah i didnt shake em, ive only ever shaken when like a quarter of jar is colonized.this whole situation is so frustrating lol that was my last syringe. im just trying to trip my face off for a weekend
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: No growth in WBS [Re: vaneazy]
#18925483 - 10/03/13 11:50 AM (10 years, 3 months ago) |
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Quote:
vaneazy said:
the lids i made,   
Your lids are kind of a hot mess but if there is no growth at all after two weeks, contams or otherwise, I would suspect the syringe is no longer viable.
Do you have another one you can use? I'd use it.
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vaneazy
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Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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how/why are the lids a mess? just because of the felt being taped on with micropore? the injection hole is just rtv silicone
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Yea, I would assume bad syring also. He never did answer where or how he sourced his spores. My guess is still contamination from the filter construction. You need two layers of tyvek between micropore tape in order for that to make a viable filter and also because not every contaminate to the mushroom culture is visible.
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vaneazy
Stranger

Registered: 09/20/12
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Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: hamloaf]
#18925589 - 10/03/13 12:11 PM (10 years, 3 months ago) |
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oh sorry, i got they syringe from a sponsor, i would say who but i know ill get yelled at haha and i thought if you were using tyvek you didnt even need the tape? when did everyone start hating on micropore tape? lol when i was first researching before i got my supplies i remember people saying they liked the tape since it was cheap/easy to find/ fairly reliable
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: hamloaf]
#18925597 - 10/03/13 12:14 PM (10 years, 3 months ago) |
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I just thought that it's perhaps the filter being made from micropore tape was too porous and has let the WBS dry out over the first few days to the point at which myc and any contams could not germinate on it.
Quote:
oh sorry, i got they syringe from a sponsor, i would say who but i know ill get yelled at haha and i thought if you were using tyvek you didnt even need the tape? when did everyone start hating on micropore tape? lol when i was first researching before i got my supplies i remember people saying they liked the tape since it was cheap/easy to find/ fairly reliable
IMO, micropore tape is good as extra protection on a PF jar's injection ports. It's polyfill or tyveck that is "tight" enough to keep moisture in, allow for gas exchange, and resist trans-filter contamination if the filter does get wet.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: No growth in WBS [Re: vaneazy]
#18925627 - 10/03/13 12:22 PM (10 years, 3 months ago) |
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Nobody is hating on micro pore tape, kid. You just have to understand that micropore tape alone is not a viable filtering material for grain jar lids. Micropore tape is reccomeded to be used when getting started with brf cakes. It's not even needed for brf cakes if your vermiculite layer is dry.
Either way the problem stems from a bad syringe or bad lid filter construction. Best bet is to start over. Hit up the Marketplace for new genetics.
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: hamloaf]
#18925730 - 10/03/13 12:49 PM (10 years, 3 months ago) |
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So bodhisatta, i should just take off the felt/tape and just put polyfill in the larger holes and that should suffice?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: vaneazy]
#18925738 - 10/03/13 12:52 PM (10 years, 3 months ago) |
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Yea polyfill or silicone two pieces of tyvek top and bottom. If using polyfill make sure it's stuffed very tight though. If your polyfill port is 1/4" or larger you can use it for injecting through with a needle too but I still like to use the SHIPs.
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Chilled
Out


Registered: 04/21/12
Posts: 261
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Last seen: 4 years, 8 months
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Re: No growth in WBS [Re: vaneazy]
#18925864 - 10/03/13 01:23 PM (10 years, 3 months ago) |
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I too have had a very similar problem to this and finally solved it, which was the pressure cooker not holding enough pressure so not raising the water boiling point enough.
Also, did you get the needle red hot when knocking jars up? Your prep sounds fine but this is the hit and miss you play with MS and syringes, would love to get some isolates and use those instead of MS syringes.
Edited by Chilled (10/03/13 01:25 PM)
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vaneazy
Stranger

Registered: 09/20/12
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Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: Chilled]
#18925891 - 10/03/13 01:28 PM (10 years, 3 months ago) |
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yeah, the needle get red hot, accidentally almost melted the plastic at the base of the needle too xD lol im not sure if this is correct but my PC has the rocker thing atthe top instead of a gauge but the 15 pound pressure rocker does bounce back and forth during PCing... does this always mean its reached 15psi? ill be super upset if i have to buy another pressure cooker haha
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Chilled
Out


Registered: 04/21/12
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Re: No growth in WBS [Re: vaneazy]
#18930755 - 10/04/13 11:21 AM (10 years, 3 months ago) |
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Where did you get the PC from? Also counting after you have got needle that hot to 10 for it to cool a little before using is good practise too. Also shacking the syringe as hard as you can for a period is good idea.
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MoGrow
Lysergic Funeral Procession



Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 1 year, 2 months
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Quote:
FrankHorrigan said:
Quote:
vaneazy said:
the lids i made,   
Your lids are kind of a hot mess but if there is no growth at all after two weeks, contams or otherwise, I would suspect the syringe is no longer viable.
Do you have another one you can use? I'd use it.
Thats what i say, i dont know when you ordered your syringe but ive read posts about not ordering during the summer because mail trucks get hot as shit. Im with frank on the shot spores, if contam is your issue you will see it. On the subject of lids, there are many ways you can set up a lid but id be afraid of taping anything to the inside of my lids, for fear that the shaking my grains would pull the filter off... but thats just me. GL.
-------------------- Trades Current Contest! My current mono (pics) Ghetto Bulk Dehydrator Tek How I Made My Alcohol Lamp Oeric McKenna said: "My innoculant is heavily contaminated with mushroom mycelium"
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: No growth in WBS [Re: vaneazy]
#18931104 - 10/04/13 12:53 PM (10 years, 3 months ago) |
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Quote:
vaneazy said: how/why are the lids a mess? just because of the felt being taped on with micropore?
Yes 
I would use silicone to attach the filters, much more reliable.
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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i used the tape so it would be easy to remove the felt when it started getting worn. I figured pulling it with the rtv would be a lot more difficult. guess ill have to switch it up lol
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: No growth in WBS [Re: vaneazy]
#18931668 - 10/04/13 03:09 PM (10 years, 3 months ago) |
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I mean, are you worried about the plastic getting messed up? Silicone peels off really easily.
If you are worried about the filters themselves, don't be...I wouldn't trust the felt when it starts to look worn anyhow. Plus the filter is going to wear out long before the lid does.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
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Actually on the plastic lids the silicone peels off a little too easily. It hear it helps if you scuff the lid a little with sandpaper.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
psillyshroomer said: Actually on the plastic lids the silicone peels off a little too easily. It hear it helps if you scuff the lid a little with sandpaper.
I hear this a lot but I'll be honest, out of my 150-odd plastic lids that I've used for years...I've not had a single issue with the silicone peeling off.
I could definitely see it happen though.
I recommend using grey RTV silicone for attaching the filters, this type is very rigid when it cures.
Also, use twice as much silicone as you'd think you would need. I see people with little tiny beads of silicone in their lids and shake my head.
I'll get my lid write up done soon, it's simple enough and I have my light box now...I'm just lazy
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Sanding the area where your going to adhere the filter disk makes the disks stick better to the plastic.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 49 minutes, 26 seconds
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Quote:
FrankHorrigan said:
Quote:
psillyshroomer said: Actually on the plastic lids the silicone peels off a little too easily. It hear it helps if you scuff the lid a little with sandpaper.
I hear this a lot but I'll be honest, out of my 150-odd plastic lids that I've used for years...I've not had a single issue with the silicone peeling off.
I could definitely see it happen though.
I recommend using grey RTV silicone for attaching the filters, this type is very rigid when it cures.
Also, use twice as much silicone as you'd think you would need. I see people with little tiny beads of silicone in their lids and shake my head.
I'll get my lid write up done soon, it's simple enough and I have my light box now...I'm just lazy 
Lazy fucker
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