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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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No growth in WBS
#18910674 - 09/30/13 10:27 AM (10 years, 3 months ago) |
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So, I noced up 7 WBS quart jars with PESA about 2 weeks ago. Literally, no growth at all for two weeks?! I see/smell nothing but WBS when inspecting the jars. I have 2 theories why this may have happened. 1) i used SHIP's to noc the jars up and for GE I used EZ felt but instead of the RTV silicone ( i couldnt getthe silicone to stick the felt on for some reason) I used micropore tape to hold the felt to the lid 2) the only otherthing i could think that would hinder growth would be the storage of the syringe. I never opened the plastic bag the syringe came in but it was in the back of my closet for a few months. Although it never got that warm in the closet could this kill the spores? I know it should have been in the fridge but yeah theywerent kept there lol.
Any input on these 2 theories or why there is no growth showing? thanks for the help guys
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: vaneazy]
#18910851 - 09/30/13 11:23 AM (10 years, 3 months ago) |
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It's probably some error in the prep, Post a pic of the jars and tell us how your preped the jars too.
The syringes go through the mail they're fine in your cabinet.
The ez felt could have introduced contamination it's hard to say how good you did with the GE port though
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Dr. FunkNShine
Funkalicious
Registered: 11/20/12
Posts: 293
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Sometimes it might take that long, but not all the time. How are you able to really smell them?
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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i can post pics but they just look like WBS in jars lol no growth of anything. i prepped them by rinsing the grains until the water ran clear, let them soak for 24hrs with some added gypsum, brought them to a simmer, let them dry. passed the toilet paper test. Put them in quart jars, PCed for 120 minutes with foil on top of them. the lids have a tiny hole with rtv on both sides for inoculation and for GE i cut squares of ezfelt and connected them to the lid with micropore tape. im not home right now but if its necessary ill post some jar pics later although like i said they will just be pics of jars with water marks on them from the PC with some WBS in them
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
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Re: No growth in WBS [Re: vaneazy]
#18910895 - 09/30/13 11:32 AM (10 years, 3 months ago) |
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Drfunk i can smell the faint smell of wbs through the ge felt but i also cracked a single jar to see if it smelled funny but just wbs i figured sometimes it takes a while im just surprised/kind of pissed im not seeing any growth even from contams lol
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Rockhound
The Rockweiler



Registered: 01/19/13
Posts: 664
Loc: hell creek anticline
Last seen: 8 years, 7 months
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Re: No growth in WBS [Re: vaneazy]
#18910913 - 09/30/13 11:37 AM (10 years, 3 months ago) |
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Throw out the jar you 'cracked' open, and don't do that again. There is no point in closing up that jar and expecting anthing but contam's.
-------------------- Rocks speak to me, and tell me this: The Hell Creek formation is a gigantic slab of rocks that covers several western states. It contains an account of the dinosaurs' demise. In the late Cretaceous period, the first Cannabis species appear, and soon after, all the non-bird dinosaurs disappeared. Obviously, marihuana killed the dinosaurs. That giant meteor that smacked the yucatan peninsula right afterwards, coincidence.
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: Rockhound]
#18910958 - 09/30/13 11:48 AM (10 years, 3 months ago) |
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theres a chance of contam but i mean i dont see any harm in keeping it around just to see if i see any growth. i only had the lid cracked for a couple seconds and it was in a clean room... as soon as i see signs of contam its gone though i know im not supposed to open them at all but after 2 weeks of nothing showing i had to check
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Rockhound
The Rockweiler



Registered: 01/19/13
Posts: 664
Loc: hell creek anticline
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Re: No growth in WBS [Re: vaneazy]
#18910989 - 09/30/13 11:54 AM (10 years, 3 months ago) |
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Your prep was identical to mine, and it works will for me. I suspect the filter isn't a great seal, or time and patience might fix everything. My guess is on patience.
-------------------- Rocks speak to me, and tell me this: The Hell Creek formation is a gigantic slab of rocks that covers several western states. It contains an account of the dinosaurs' demise. In the late Cretaceous period, the first Cannabis species appear, and soon after, all the non-bird dinosaurs disappeared. Obviously, marihuana killed the dinosaurs. That giant meteor that smacked the yucatan peninsula right afterwards, coincidence.
Edited by Rockhound (09/30/13 11:55 AM)
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: Rockhound]
#18910993 - 09/30/13 11:55 AM (10 years, 3 months ago) |
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hopefully the time and patience will pay off haha thanks though
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OregonMushys
Rye Wata Whippin



Registered: 09/09/13
Posts: 280
Last seen: 6 years, 4 months
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Re: No growth in WBS [Re: vaneazy]
#18911148 - 09/30/13 12:37 PM (10 years, 3 months ago) |
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Im havin problem with my 2yr old spore syringes not germinating anything. Take spore prints from every most recent grow, and/or make agar slants or you'll have to order spores every 2 yrs lol.
-------------------- Ps. Cubensis Ps. Cyanescens Ps. Stuntzii *GrowLog*
    
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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im just gonna give this a bump to see if the night crowd at the shroomery has anything to say lol
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: vaneazy]
#18914839 - 10/01/13 07:03 AM (10 years, 3 months ago) |
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Everything that can be said has been said unless you want to post pictures, but if you think your jars are A-OK then don't post them and figure out where things went wrong?
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: No growth in WBS [Re: vaneazy]
#18914895 - 10/01/13 07:29 AM (10 years, 3 months ago) |
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Where and how did you source the spores that you inoculated your jars with? I think you have a contamination though because you used micropore tape to adhere the filters to the lids instead of silicone. Micropore tape has very large pores that contamination to the mushroom culture can easily get through and is why when adhering filter disks to lids silicone is used because the silicone creates an air tight seal allowing air to only pass the filtering material.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: hamloaf]
#18915526 - 10/01/13 10:44 AM (10 years, 3 months ago) |
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Quote:
hamloaf said: Where and how did you source the spores that you inoculated your jars with? I think you have a contamination though because you used micropore tape to adhere the filters to the lids instead of silicone. Micropore tape has very large pores that contamination to the mushroom culture can easily get through and is why when adhering filter disks to lids silicone is used because the silicone creates an air tight seal allowing air to only pass the filtering material.
This, is probably exactly why he has problems. But heh he don't wana show us his jars.
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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whoa bodhisatta just relax lol i was getting around to posting them ive just had a lot on my plate the last couple days and didnt have much time
the lids i made,   and theres the jars. the lighting was weird but theres literally nothing growing in any of the jars, not even contams. And i even opened the same jar i opened last time and it still just smells like straight wbs. the picture of the single jar is kind of fuzzy but thats just water marks ( i guess thats what happens in a PC )on the outside
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: vaneazy]
#18922534 - 10/02/13 05:23 PM (10 years, 3 months ago) |
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oh sorry, i forgot to ask. Ive used just micropore tape for my GE ( with 4 holes in metal lids)for my first 2 grows and never had any problems. I thought a lot of people used micropore tape with success?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,504
Loc: USA
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Re: No growth in WBS [Re: vaneazy]
#18923003 - 10/02/13 07:22 PM (10 years, 3 months ago) |
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I use micropore tape all the time with success.. cloth kind. Ive never used the paper kind
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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yeah same here, supa. and these guys just talkin ish haha I actually think the paper kind is called something other than micropore tape for some reason though lol i cant remember where i saw that
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vaneazy
Stranger

Registered: 09/20/12
Posts: 332
Last seen: 10 years, 1 month
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Re: No growth in WBS [Re: vaneazy]
#18925185 - 10/03/13 09:59 AM (10 years, 3 months ago) |
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anything bodhisatta?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No growth in WBS [Re: vaneazy]
#18925224 - 10/03/13 10:12 AM (10 years, 3 months ago) |
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Bad syringe? I'm at a loss. The micropore tape thing is weird I would think you would have at least contamination growing if that was the problem. Did you shake them up after you injected the syringe?
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