Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
BRF cake question
    #18904405 - 09/28/13 08:29 PM (10 years, 4 months ago)

I would like to do an agar to grain transfer. I'd like to use a BRF cake.

Instead of using a dry verm layer, can I use micropore tape over the breather holes and have the same level of protection from contams?


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Edited by MarcusFreeman (09/28/13 08:29 PM)


Extras: Filter Print Post Top
Offlinemushbecrazy
newbee
 User Gallery

Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
Re: BRF cake question [Re: MarcusFreeman]
    #18904431 - 09/28/13 08:34 PM (10 years, 4 months ago)

Never use a dry layer. Just throw you some mycelium in it. Shake and wait.


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


Extras: Filter Print Post Top
OfflineCpt.Crunch
Humanitarian


Registered: 08/09/13
Posts: 353
Last seen: 9 years, 7 months
Re: BRF cake question [Re: MarcusFreeman]
    #18904902 - 09/28/13 10:35 PM (10 years, 4 months ago)

Just make a simple spore syringe, what's the problem??

Quote:

Instead of using a dry verm layer, can I use micropore tape over the breather holes and have the same level of protection from contams?




No, if you don't want your cakes to contaminate then you need to put your verm layer their to protect it. Fine verm works the best, but coarse will do if that's all you can get.


Extras: Filter Print Post Top
Invisiblestevo

Registered: 04/11/05
Posts: 5,100
Re: BRF cake question *DELETED* [Re: Cpt.Crunch]
    #18905045 - 09/28/13 11:13 PM (10 years, 4 months ago)

Post deleted by stevo

Reason for deletion: .


Edited by stevo (03/13/20 10:58 AM)


Extras: Filter Print Post Top
Offlinekinkaku
I AM THE LAW!!!!
Male User Gallery


Folding@home Statistics
Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
Re: BRF cake question [Re: stevo]
    #18905075 - 09/28/13 11:23 PM (10 years, 4 months ago)

are you asking if you need the verm layer on cakes or grains? grains no, cakes highly recomended:thumbup:


--------------------


Extras: Filter Print Post Top
Offlinemushbecrazy
newbee
 User Gallery

Registered: 09/23/13
Posts: 158
Loc: alabama
Last seen: 10 years, 4 months
Re: BRF cake question [Re: kinkaku]
    #18905968 - 09/29/13 06:51 AM (10 years, 4 months ago)

Quote:

kinkaku said:
are you asking if you need the verm layer on cakes or grains? grains no, cakes highly recomended:thumbup:






I thought he was talking about mycelium from a brf jar into grain. If thats the case then no dry layer is wanted itvwill just get mixed in durring shaking the jars


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 5 days
Trusted Cultivator
OG Cultivator
Re: BRF cake question [Re: MarcusFreeman]
    #18906117 - 09/29/13 07:55 AM (10 years, 4 months ago)

Quote:

MarcusFreeman said:
I would like to do an agar to grain transfer. I'd like to use a BRF cake.






This doesn't make sense.  I think you need to brush up on your terms.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: RogerRabbit]
    #18906404 - 09/29/13 10:00 AM (10 years, 4 months ago)

I guess I need to clarify- and what I am asking about may not be something that people do.

I recently did my first grow. It went really well for me. I used the PF tek from RR's videos. I followed the tek to a 'T' and was pretty happy with the results.

When I started the grow, I used spore prints, made spore syringes, and inoculated the cakes with a MS solution. 

From my grow, I picked a few mushrooms that were exceptionally fast and large growers, and I cloned them to agar using some of the tissue from the inner stipe.

The cultures are growing beautifully, and now I would like to use them in my next batch of cakes.

So, now to my main question. I would like to know what is the best method to transfer that culture to a PF style cake. I had a friend tell me that I can make PF style cakes, but leave the dry verm off the top of the cake and put micropore tape over my breather holes. That way, I can drop the wedge on top of the PF cake and it will colonize from there. This was not part of the TEK I was following, so I was unsure if it will work.

I am also open to other suggestions about transferring agar culture to a PF style cake.

Thanks for the input so far!


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: MarcusFreeman]
    #18910260 - 09/30/13 07:42 AM (10 years, 4 months ago)

Alrighty then. Ill just try it and find out for myself.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
Offlinespoonfed
Stranger
Registered: 09/28/13
Posts: 15
Last seen: 10 years, 4 months
Re: BRF cake question *DELETED* [Re: MarcusFreeman]
    #18910279 - 09/30/13 07:51 AM (10 years, 4 months ago)

Post deleted by spoonfed

Reason for deletion: .



Edited by spoonfed (09/30/13 07:52 AM)


Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: spoonfed]
    #18910530 - 09/30/13 09:26 AM (10 years, 4 months ago)

That's pretty much the plan. I will be putting micropore tape over the inoculation holes. If I use the micropore tape, and treat the PF cake like a grain jar, i'm hoping to pull this off.

If it fails, darn. I'll make a grain jar. I want it to work with the cake because they are more "set it and forget it"- IMO


I guess I could try making a liquid culture from the agar, but I hear that LCs are a complete crap shoot.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
Trusted Cultivator
Re: BRF cake question [Re: MarcusFreeman]
    #18910671 - 09/30/13 10:25 AM (10 years, 4 months ago)

If you want to use a agar wedge to inoculate a PF jar make a lid with only a gas exchange hole on it. The hole is about 1/4 inch and you stuff it very tight with polyfil or two layers of tyvek glued on. Then you can put the wedge into the jar in a glove box or SAB. In this case you wouldn't use a verm barrier. I'm curious to how you have an agar wedge colonized but don't just do grains?


Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: bodhisatta]
    #18912119 - 09/30/13 05:03 PM (10 years, 4 months ago)

I have done some agar to grain as well. If I was trying to make a large grow, id probably put more energy into grains and monotubs.

In all honesty, my first grow produced more than enough for myself and any friends(if I had any) to munch on for months.


The cakes were simple enough to prepare, and I have all the materials except tyvek. When I prepared grains, it took much longer and stunk up the house.

Also, I have not really researched the different grain teks. I researched agar early on because I got into cultivation from collecting wild samples, printing them, then making a spore solution and growing it out on agar. I got to practice transferring mycelium in sterile conditions, fighting molds, and learning patience before I messed with cultivation.

I'll work my way up to grains, but for now I am enjoying the small scale cultivation


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
Trusted Cultivator
Re: BRF cake question [Re: MarcusFreeman]
    #18912301 - 09/30/13 05:46 PM (10 years, 4 months ago)

In that case use agar to make a LC and then test that LC on one cake and if it's good then use it on more.


Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: bodhisatta]
    #18912543 - 09/30/13 06:42 PM (10 years, 4 months ago)

Better yet, I'll do both.

I haven't made LCs before, so I'll have to read up on it.

This is one of my first grain jars:


I have three of these, which I made last week. I used a colonized agar wedge to transfer the mycelium. This species is not cubensis and will require some special fruiting conditions. I'm not expecting a complete success, but I don't think it will end in complete failure either.

Those are a wild Psilocybe that grow in my area, and it would be fun to grow them inside.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
Offlinespoonfed
Stranger
Registered: 09/28/13
Posts: 15
Last seen: 10 years, 4 months
Re: BRF cake question [Re: MarcusFreeman]
    #18913758 - 09/30/13 10:57 PM (10 years, 4 months ago)

Very Cool man!

Quote:

MarcusFreeman said:
That's pretty much the plan. I will be putting micropore tape over the inoculation holes.




I read that on some thread that RR does just that, Flame's needle and goes through tape...although your not using a needle.

Quote:

MarcusFreeman said:

If it fails, darn. I'll make a grain jar. I want it to work with the cake because they are more "set it and forget it"- IMO


I guess I could try making a liquid culture from the agar, but I hear that LCs are a complete crap shoot.




:toast: To trial and error! Why not, it is supposed to be a fun hobby! :laugh: 

Best of luck!


Edited by spoonfed (10/02/13 08:27 PM)


Extras: Filter Print Post Top
InvisibleMarcusFreeman
 User Gallery

Registered: 09/16/13
Posts: 376
Re: BRF cake question [Re: spoonfed]
    #18917037 - 10/01/13 04:14 PM (10 years, 4 months ago)

Thanks man!

I had a little spell of the ADD and got a little off path today, but it's probably for the better.

I picked up a big ass bag of rye grass seed and gypsum. After reading up on RGS, it seems WAY easier and cleaner than WBS. The prep of the WBS is what was turning me off of grain- soaking, boiling, straining, drying, and then PC for two hours... thats a lot of time and energy.


Im still going to do the other experiments too, but the RGS seems easy enough. Ive been working on that today. Tomorrow- time for liquid culture learning.


The cake with out the verm layer wont take more than 5 mins to prep, so ill probably work on that tonight.


I had two left over quarts of wbs that I was going to use on the "wild psilocybe" project, so I used half a wedge of the cloned cube in each jar, instead.

I have 2 more plates of the same clone that ill use on the RGS, LC, and vermless PF cake.

I guess ill find out which method I like best via trial and error. Techniques that work well for one may not be a great match for someone else. Im thinking that I'll let other folks enjoy the WBS. :smile:


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* BRF cake "goo" Sportello 416 4 03/08/18 09:52 PM
by Sportello
* Spawning sheep poop w/ brf cakes indicaz 4,345 9 10/04/09 09:18 PM
by bike__52
* multi BRF cake casing matamore 6,086 5 12/28/12 10:26 AM
by twistedty
* Spawning from BRF cakes. Juggles 8,127 7 01/24/02 10:03 AM
by Anonymous
* Can I use brf cakes for wheat straw cmb911 2,884 4 02/24/02 02:15 AM
by Zebedee
* SPAWNING POOP?! With BRF cakes indicaz 3,324 5 08/19/18 04:18 AM
by mushpunx
* Can I grind up BRF cakes & mix w/ Compost skoal 4,616 3 06/25/20 05:11 PM
by Cegda
* brf cakes -> verm casings. Where next aenima 3,065 2 03/19/02 03:17 AM
by aenima

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
869 topic views. 25 members, 179 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 12 queries.