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Offlinenasser
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agaricus on agar updated !!!! with pics
    #18910001 - 09/30/13 05:11 AM (10 years, 3 months ago)

hi am new with white button
i tried to clone a store bought agaricus bisporus on pda and mea and on cardboard the problem is that hte myc is growing on the tissue and no growth on agar its some kind of fuzzy little growth on the agar what is the problem

one of the plates is from spores after 4 months when i transfered it to grain the same problem occurred the myc is growing on agar but not the grain


Edited by nasser (10/04/13 02:05 AM)


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Offlinethiotimoline
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Re: agaricus on agar problems????? [Re: nasser]
    #18913315 - 09/30/13 09:32 PM (10 years, 3 months ago)

Mycelium sometimes takes a while (days to weeks depending on species, age of culture, and other things) to adapt to a new food source, and then grow rapidly once it decides to stop being picky. In the meantime, fuzz growing only on the tissue is absolutely normal.

How long has the tissue been on the agar? One problem with cloning store-bought mushrooms is that they start a culture from spores and grow it out through as many generations of spawn as they can get away with before putting it on bulk substrate and giving it "fruiting conditions." With age, a culture may get pickier about what food it will accept, or may refuse to spread or grow mushrooms at all. (But this does not explain problems with a culture you have started yourself from spores.)

If there is fuzzy growth on the agar not near the tissue, either (1) the tissue touched that part before landing in its final position, lost a few cells there, and those are now growing or (2) you have mold contamination. I can't quite tell what's going on from these pictures.


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Offlinecubenpete
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Re: agaricus on agar problems????? [Re: thiotimoline]
    #18913628 - 09/30/13 10:31 PM (10 years, 3 months ago)

What kind of agar are you using? I ran into this problem, mycelium growing on tissue only and dying off. Come to find out, some agar agar bought in Asian markets contain fungicide to ensure shelf life. I also ran into this problem when trying to make a draft cider from store bought apple juice... it had arsenic in it and killed my yeast rapidly. Live, grow and learn.


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Offlinedrake89
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Re: agaricus on agar problems????? [Re: cubenpete]
    #18913909 - 09/30/13 11:42 PM (10 years, 3 months ago)

i'm guessing pic 3 is from spores.  i don't have much experience with agaricus, but don't bother with store bought shrooms.  LC from a vendor is the best recipe for success.


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Offlinenasser
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Re: agaricus on agar problems????? [Re: thiotimoline]
    #18914134 - 10/01/13 12:52 AM (10 years, 3 months ago)

Quote:

thiotimoline said:
Mycelium sometimes takes a while (days to weeks depending on species, age of culture, and other things) to adapt to a new food source, and then grow rapidly once it decides to stop being picky. In the meantime, fuzz growing only on the tissue is absolutely normal.

How long has the tissue been on the agar? One problem with cloning store-bought mushrooms is that they start a culture from spores and grow it out through as many generations of spawn as they can get away with before putting it on bulk substrate and giving it "fruiting conditions." With age, a culture may get pickier about what food it will accept, or may refuse to spread or grow mushrooms at all. (But this does not explain problems with a culture you have started yourself from spores.)

If there is fuzzy growth on the agar not near the tissue, either (1) the tissue touched that part before landing in its final position, lost a few cells there, and those are now growing or (2) you have mold contamination. I can't quite tell what's going on from these pictures.






its now 7 days i did the clone on 23 Sep
and the myc is only on the agar so i don`t think its a contamination

mr. cubenpete 
am using ordinary PDA and MEA with same results
  on cardboard ther is a deferent growth its better but the camera is bad so i will upload the photo next week till i buy another one
now should i try it on grain at this stage or just wait  ?????


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Offlinenasser
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Re: agaricus on agar problems????? [Re: drake89]
    #18914149 - 10/01/13 12:58 AM (10 years, 3 months ago)

the 3rd picture is from spores and it took too long time several months  but i dont know what to do with it it did not succeeded on grain


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Offlinenasser
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Re: agaricus on agar problems????? [Re: nasser]
    #18929346 - 10/04/13 02:05 AM (10 years, 3 months ago)

thes new pics after the myc started growing on agar not forgetting the cardboard its really amazing i never believed in cloning agaricus on cardboard hope its not a contamination bu the mys started from the tissue i think its just fine it needs more time.

its now day 11 and its cool i can`t wait to see it on grain any advice for a grain recipe


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Life is no more than a piss of shit
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Offlinethiotimoline
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Re: agaricus on agar problems????? [Re: nasser]
    #18931540 - 10/04/13 02:45 PM (10 years, 3 months ago)

Looking good!

My grain recipe is:
-rinse whole rye grains (a cup of them per quart jar)
-soak overnight
-boil for 10min
-drain them, shaking a bit to help steam evaporate
-distribute evenly into jars
-pressure cook for 90min

Sometimes I've added some gypsum, but it didn't seem to make much difference.


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