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Offlinedark_nemesis
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Round 2
    #18908926 - 09/29/13 08:54 PM (10 years, 3 months ago)

So awhile ago i posted a couple of jars using a tek that wasn't very good. I got alot of negative responses. So i scrapped that project, and started anew. I followed the PF Tek pretty much to the T. Minus two things. Instead of using half pint jars, i used 1/4 pint jars. I also did not use as big of a fruiting chamber. I am using  the shotgun fruiting chamber. Its been about 2 and a half weeks and i believe i am at about 85-90 percent colonization. I have five jars, but each with a different strain. 1 Has ecuador, 1 penis envy, 1 cambodian, 1 s. American, and 1 has amazon. Ill upload some pics when i can(im posting from my phone.) I do have one question though, would having all five cakes each with a different strain in one fruiting chamber cause any problems? I was worried that maybe you could only have one strain per chamber, but was unsure if it would matter. Any comments on the subject?


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Offlinegeazerpleaser
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Re: Round 2 [Re: dark_nemesis]
    #18908939 - 09/29/13 08:56 PM (10 years, 3 months ago)

they should all be fine together in the same fruiting chamber :mushroom2:


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Offlinedark_nemesis
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Re: Round 2 [Re: geazerpleaser]
    #18909002 - 09/29/13 09:09 PM (10 years, 3 months ago)

Okay, I was just wanting to make sure. I was worried that with the different strains that there might be a problem with the mushrooms, when the veils break having spores cross contaminate or anything. I really didn't think there would be because considering that while.colonizing, there is more probability that the mycelium can fight off any other things trying to take a foothold. So i didnt think that other spores would do anything. Besides that, the cakes are already colonized so i didnt think that anything would come of it.


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Offlinegeazerpleaser
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Re: Round 2 [Re: dark_nemesis]
    #18909052 - 09/29/13 09:17 PM (10 years, 3 months ago)

as long as there fully colonized on there own cakes theres are no chance of spores from one species taking over the other since they'll be fully developed 

Oh and you should make a a grow log, I'd love to see all those different types of cubes!


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Offlinedark_nemesis
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Re: Round 2 [Re: geazerpleaser]
    #18909109 - 09/29/13 09:29 PM (10 years, 3 months ago)

Actually I have kind of started an elementary grow log. I have the date of inoc, and the date i started to see the white fuzz. I have been checking the jars just about every other day and havent really logged the mycelium growth. What exactly should i have down in my grow log? This is my first grow by myself. I helped a friend a loooooong time ago grow some and he kind of explained it.to the process to me so i started doing some research. Obviously i have started my own grow, but dont exactly know what makes a "good" grow log.


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Offlinegeazerpleaser
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Re: Round 2 [Re: dark_nemesis]
    #18909134 - 09/29/13 09:35 PM (10 years, 3 months ago)

well everyone has there own way of doing a grow log but the most common things you should put in them are teks you've used, time stamps (how long it takes for the mycelium to show, how long for them to be able to be birthed, how long did they take to be able to fruit), the weigh of the harvested fruit from each cake, and above all PICTURES.


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Offlinegeazerpleaser
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Re: Round 2 [Re: geazerpleaser]
    #18909170 - 09/29/13 09:44 PM (10 years, 3 months ago)

Also Id love some spore samples of some of those strains when they fruit, if you're so kind to share :wink:


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Offlinedark_nemesis
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Re: Round 2 [Re: geazerpleaser]
    #18909228 - 09/29/13 09:54 PM (10 years, 3 months ago)

okay thanks for the info. Like i said i started a log, but ill get on myself about taking better noted and taking alot of pictures. As i said, ill post some pictures when i get a chance.


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18910561 - 09/30/13 09:35 AM (10 years, 3 months ago)

im not sure what you mean by you'd love some spore samples good sir. Again, sorry im an extreame noob.


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18912533 - 09/30/13 06:40 PM (10 years, 3 months ago)

okay, so here are the pictures of the jars at about 85% or so colonization.














I'm thinking in roughly about 1 1/2 weeks I'll start to see some pinning, and at that time I am going to get my FC up and running. I'll also take some more pictures of jars as I start to see pinning. I'll take some pictures of the FC and post those as well, so you can see what I'm working with. Again its smaller then the FC in the Tek vids, but so are my jars so I figured if I downsized one, the other wouldn't matter if it was also downsized.


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Offlinegeazerpleaser
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Re: Round 2 [Re: dark_nemesis]
    #18912580 - 09/30/13 06:48 PM (10 years, 3 months ago)

Quote:

dark_nemesis said:
im not sure what you mean by you'd love some spore samples good sir. Again, sorry im an extreame noob.




lol idk if this is sarcasm or not, but what I ment was when they are ready to be picked they should start to drop spores from under there caps  and some people cut off the caps off of the stems and place them on aluminum paper or regular paper and millions of spores fall out leaving a black print on the paper, so what I meant was you should send me one of those prints :grin:

EX:


Edited by geazerpleaser (09/30/13 08:10 PM)


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Offlinetybojengles
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Re: Round 2 [Re: geazerpleaser]
    #18912978 - 09/30/13 08:08 PM (10 years, 3 months ago)

:whathesaid:


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Offlinedark_nemesis
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Re: Round 2 [Re: tybojengles]
    #18918338 - 10/01/13 09:05 PM (10 years, 3 months ago)

Okay guys, here is an update on my jars. I've taken pictures with a better camera(obviously my phone is nothing compared to my actual camera). Oh and I've read that flipping the jar over on its top about a week before birthing and the D&R to help allow gas exchange. So I've recently flipped the jars over.

My Fruiting Chamber with the jars inside:


I don't have the perlite inside because I am not currently at that stage. As I said, they are at about 90% colonization at this point which you can see from my pictures below.

South American:






Penis Envy:






Cambodian:






Ecuador:






Amazon:






All The Babies in One Shot:


As you can see from the pictures, there is moisture build up inside the jars. This is my first grow, so I am a little worried about the moisture inside the jars. What do you guys have to say on the subject? I know that a little build up is normal, but I'm not quite sure what "normal" is.


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OfflinePussyFart
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Re: Round 2 [Re: dark_nemesis]
    #18918368 - 10/01/13 09:11 PM (10 years, 3 months ago)

It's perfectly normal for them to condensate, as they are constantly generating heat.

And we know that condensation is from a temperature differential, so yea it's normal.


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Offlinedark_nemesis
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Re: Round 2 [Re: PussyFart]
    #18918843 - 10/01/13 10:53 PM (10 years, 3 months ago)

Oh and one more question, should I D&R before I start seeing the signs of pinning, or after? In the RR videos, he said to wait one week after 100% colonized, and then D&R , but I've also read that you should wait till you start to see pinning before removing from the jar and moving to the FC. The latter seems a little counter-productive because IMO I wouldn't think that handling the cakes after pinning would be a good idea (don't want to damage the pins/cause excessive bruising).  Also would you guys say that my assessment of the percentage of colonization is accurate based on my photos? I'm just wondering because I don't want to wait too long to D&R.

and sorry for all the "fancyness" of the text, im just messing around with the buttons up top the comment box and learning  :takingnotes:  :takingnotes:
When Should you wait to D&R
You may choose only one


Votes accepted from (10/01/13 08:50 PM) to (No end specified)
View the results of this poll



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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18920089 - 10/02/13 09:16 AM (10 years, 3 months ago)

Anyone?


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OfflinePussyFart
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Re: Round 2 [Re: dark_nemesis]
    #18920757 - 10/02/13 12:07 PM (10 years, 3 months ago)

Always give BRF cakes an extra week for consolidation.

They will just sit in the FC and consolidate if you don't.

That is a bunch of wasted misting/fanning for nothing.

Even if there are invitro pins, let them consolidate.


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Offlinedark_nemesis
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Re: Round 2 [Re: PussyFart]
    #18921556 - 10/02/13 02:17 PM (10 years, 3 months ago)

Ah okay, thank you. When I birth the cakes, I want to cut the pins off though correct? I was reading on another thread of a guy who birthed them without cutting them off, and he said he got a really slimy cap.


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OfflinePussyFart
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Re: Round 2 [Re: dark_nemesis]
    #18921570 - 10/02/13 02:23 PM (10 years, 3 months ago)

Quote:

dark_nemesis said:
Ah okay, thank you. When I birth the cakes, I want to cut the pins off though correct?



No, just dunk for 24 hours in tap water, roll/sprinkle them gently with dry verm and place them in the fruiting chamber.

The pins that are there would most likely turn out to be your biggest fruits.

Quote:

dark_nemesis said:
I was reading on another thread of a guy who birthed them without cutting them off, and he said he got a really slimy cap.



Slimy caps have nothing to do with leaving invitro pins attached.

They will be fine, all mine were when I was doing cakes.


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Offlinedark_nemesis
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Re: Round 2 [Re: PussyFart]
    #18921742 - 10/02/13 03:00 PM (10 years, 3 months ago)

Okay, thanks for the info.


Edited by dark_nemesis (10/02/13 03:00 PM)


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18929161 - 10/04/13 12:43 AM (10 years, 3 months ago)

Okay guys, its been 3 days since my last update on my jars. Thought it may be time to let you guys know how they are doing. I've just checked on them tonight and it seems like I am getting some ribbons/possibly the start of pinning(hoping :grin: :thumbup:). Here are some pics. Sorry if the pictures are kind of hard to see exactly what I am talking about, the jars I am using are all textured on the outside and make seeing things a tad bit difficult. So, I used a light to try and enhance the quality a little in some and circled what I am talking about in Red in all of them. Also it seems that some of them are easier to see what im talking about with the picture smaller then full size. :confused:

S. American:

There are actually two(2) bulges atop of the S. American jar. I'm thinking maybe knots? Seeing as I'm not 100% sure what knots are, this is obviously a guess. Any ideas?

Ecuador:


Penis Envy:


Cambodian:


Amazon:


I'm thinking that maybe tomorrow morning I'll initiate the D&R and leave it over night while I go see a concert (Bassnectar :bow2: :dancer: :rockon: ) and then when I get back, rinse my perlite, place it in my FC and then roll the cakes and place them in the fruiting chamber. Seems like a pretty solid plan. Well that's it for this update. Thanks for taking the time to read all this mumbo jumbo. Peace!


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18954793 - 10/09/13 02:52 PM (10 years, 3 months ago)

Okay guys here is another quick update. I've done the D&R, and I've got my mushrooms in the FC. I've been misting and fanning about 3-6 times a day. I have some pinning/mushrooms growing. :laugh: Here are some pictures.

Ecuador:


Amazon:


Cambodian:


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Offlinespacechildo
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Re: Round 2 [Re: dark_nemesis]
    #18954849 - 10/09/13 03:06 PM (10 years, 3 months ago)

cut down the sizes of your alu foil.
It's hindering much of the moisture drawn up through the perlite.
Just leave maybe 1/2" in a circle outside of the cakes edges.

Besides that, it's looking mighty fine! :thumbup:


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18955069 - 10/09/13 04:01 PM (10 years, 3 months ago)

Okay thank you for the info, I'll trim them back right now.


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18963628 - 10/11/13 11:54 AM (10 years, 3 months ago)

Okay, so another quick update, 3 of my cakes have started getting some really promising results. My other two (P.E. and S. Amer.) seem to be slow to fruit. I want to say that i read that P.E. is slow to fruit, but not sure about S. Amer. Any ideas? but here are the pics of the three cakes that are really taking off (sorry for the slightly blurry pics):

Cam:


Ecuador:


Amazon:


Thanks for reading!


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18969583 - 10/12/13 07:23 PM (10 years, 3 months ago)

Here are some new pics of these bad boys. I really was not expecting them to grow this fast. I'm killer excited about this. :laugh: :smile:

Ecuador:


Cambodian:


Amazon:


Top-Down veiw:


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18971760 - 10/13/13 10:59 AM (10 years, 3 months ago)

Okay, so let me fill you guys in a little bit. Where i have been growing these bad boys is in my spare bedroom that hold my 75 gal. fish tank. Well I have been having a bad habit of not shutting my fish tank lights off at night. WELLLLL last night I actually shut them off while I went to bed. HOLY SHIT! I seriously did not know that shutting off the light could effect these guys soooo much. They have practically doubled in size. Here are some pictures. I posted the ones up there ^ last night. Here they are this morning:

Top-Down View:


Cam:



Eq:



Amaz:


Oh and my other two cakes have finally started pinning! YAY! :rockon:

P.E.


S. American:


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18972136 - 10/13/13 01:13 PM (10 years, 3 months ago)

So my first veil just ripped. Should I harvest? Its only one that's torn though. Its also attached to like three other at its base so I'm not sure how to go about it. Any ideas?


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18972215 - 10/13/13 01:39 PM (10 years, 3 months ago)

Anyone?


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InvisibleOneiricOutsider
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Re: Round 2 [Re: dark_nemesis]
    #18972401 - 10/13/13 02:34 PM (10 years, 3 months ago)

If you're going for highest potency/dry gram then yes, I'd harvest. If the other mushrooms are still small/growing, just take off the one with the torn veil before it drops its spores everywhere. I'd harvest the rest pretty soon afterwards though and re-dunk for the next flush. Use some clean scissors to cut at the base as close to the cake as possible.
Edit: A sharp knife might be better/more precise, especially around a cluster.


Edited by OneiricOutsider (10/13/13 02:38 PM)


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Offlinedark_nemesis
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Re: Round 2 [Re: OneiricOutsider]
    #18973616 - 10/13/13 07:37 PM (10 years, 3 months ago)

Okay thanks. I went out and bought an Exacto knife.  One more thing, I've read that I need to give my cakes a 2 day rest before i re-DNR and put back into the FC, is this true?


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OfflinePussyFart
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Re: Round 2 [Re: dark_nemesis]
    #18973621 - 10/13/13 07:38 PM (10 years, 3 months ago)

Not really needed.

Neither is the second roll.

Just dunk them and place them back in the FC.


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18974185 - 10/13/13 09:54 PM (10 years, 3 months ago)

Okay thanks. Exactly how long should i wait after the veil breaks before i try to do a spore print? I've done my first harvest of the Cam strain, and got 22.3 grams wet from about 5 mushies. I pretty much picked them as soon as the veil was completely broken from the cap and is hanging on the stipe. Is this too soon? The caps seem to have flattened a bit.
I'm currently attempting my first spore print (wish me luck), but it seems easy enough so hopefully it will go without a hitch. The cap is laying flush (flat) on the aluminum foil so I think i picked it and cut it correctly.

Since my dehydrator isn't here yet, I went out and bought a little plastic basket with decent slits in the four sides and a solid base and placed a piece of aluminum foil as the base and placed another piece of aluminum foil atop to cover it and pocked holes into it for maximum air flow. I then placed said basket on a shelve and put a fan about 1 to 1.5 feet away from it and have it blowing on it at it's maximum setting. I'm hoping that soon ill have some mushies to eat :mushroom2: .


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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18987097 - 10/16/13 04:56 PM (10 years, 3 months ago)

Dark_nemesis here, got another update for you guys. I've picked all the mushrooms from the Cam, the Ecuador, and the Amazon. I've dried them simply by putting them in a basket and placing them in front of a fan for 24+ hours(Hopefully my dehydrator will arrive either today or tomorrow).

The Amazon and the Ecuador I got 3.5 and 3.55 grams (dry) The Cam I got roughly 4.6g (dry) but only have 3.6g. I HAD to test them! :rolleyes:

Me and my girlfriend each ate .5 (it was her first time :tongue2: ) I ate just a cap that weighed .5 and my girl ate a cap and a stem that weighed .5. We both had a pretty good time. I got a really good body buzz that faded into a head high. We both got a really good case of the giggles. This lasted for roughly 1.5 to 2 hours. We weren't really trying to trip balls as much as we were trying to make sure they were actually good and some what potent. They seem to be decent considering I haven't ate shrooms in well over 2 years, and I weigh roughly 150lbs and a half gram effected me for 2 hours? Yea they are decent.

But actually I had a question for you experts here at the shroomery. My other two cakes (P.E. and South American) have been extremely slow to fruit. I have also noted that there is some blue-ing of the cake on the S. Amer. (see pics below). My girlfriend says she picked it up and rotated it for better sunlight. There also seems to be some discoloration of the base of some of the stems.(again pics below) Is this contamination or just bruising of the cakes and stems? The parts I'm questioning is the rim closest to the camera in the pics, and the area kind of below that. The stems in question are the stems are atop of the cakes. I'd hate to have to chalk it up to contam. but if it is there isn't much I can do about it.

Here are the pics of the cake in question:



Here are some more pictures of the grow as we neared harvest and at harvest:


Here are some pics of the spore prints that I got (one is a little dark because I only left it for 12 or so hours):




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Offlinedark_nemesis
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Re: Round 2 [Re: dark_nemesis]
    #18988473 - 10/16/13 09:41 PM (10 years, 3 months ago)

Anyone? I could use an answer because i have some that are ready to harvest, and if they are contaminated then I'm just going to throw the whole cake away, if they aren't, then I'm keeping them :laugh:


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InvisiblePastywhyteMDiscord
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Re: Round 2 [Re: dark_nemesis]
    #18988513 - 10/16/13 09:49 PM (10 years, 3 months ago)

It looks fine, in fact a little bluing is to be expected after pushing out some boomers like those. If the cake feels light you can rehydrate it mid flush by setting it in a saucer of water. Be sure to dunk after. Those fruits look fine, if you don't want em I'll eat em :awesomenod:

Try to take your prints under cleaner conditions, don't expose them to more open air than absolutely necessary.


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Offlinedark_nemesis
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Re: Round 2 [Re: Pastywhyte]
    #18988588 - 10/16/13 10:02 PM (10 years, 3 months ago)

Okay thank you. About the prints, just from the pics, do you think that these prints will be viable? Only reason I ask is because I don't want to be sitting on some prints and then when I go to use them, get nothing but contams.

Also I'm thinking of going bulk using a 40/50/10 ratio using coco coir/verm/coffee grounds. I've been doing some research about it on here and it seems easy enough, was going to follow this tek:

http://www.shroomery.org/forums/showflat.php/Number/14995972#14995972

How hard is bulk exactly? I'm slightly intimidated by the thought of it, but the success of the cakes gives me hope. I plan on continuing the cakes on the side so as to try to "perfect" the art of it, but am kind of hoping for a little more yield and payout of mushies :laugh:


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InvisiblePastywhyteMDiscord
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Re: Round 2 [Re: dark_nemesis]
    #18988682 - 10/16/13 10:18 PM (10 years, 3 months ago)

Bulk is not hard, pasteurize your sub, grate the cakes fine, mix it all up well. Be sure to use a spawn ratio no lower than 1-4. 1-2 is pretty good. Skip the coffee grounds until you have your pasteurization perfected. I like to do 75% coir 25% verm. Should give a good yield.

I do my prints in my SAB. This is a pretty good tek to follow.

http://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=2809570&PHPSESSID=&fpart=1#2809570

If you don't have a PC you can do the same thing in the oven at 450F for an hour. Just use foil to cover the jar instead of tyvek. I would not consider any print taken from a fruit that was grown in open air sterile but this is pretty clean for the most part. I almost never see contams on agar with spore prints taken this way.

Many people just take the print right off the roll in the SAB as the idea is that foil is pretty clean to begin with. Both can work.

No matter what I cannot stress agar enough. Prints are almost never sterile and because of this syringes are almost never sterile. The sooner you learn agar the better :super:


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Offlinedark_nemesis
The Initiate
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Registered: 09/07/13
Posts: 83
Last seen: 10 months, 8 days
Re: Round 2 [Re: dark_nemesis]
    #18988749 - 10/16/13 10:29 PM (10 years, 3 months ago)

Ok thank you again for the info. Could you possibly refer me to a good agar tek or tutorial? Also not to be a pain, but a SAB build tek? I've been wanting to make one for a little while now, but haven't really done any searching for a tek. I know I should be using the search feature, and I apologize for not. I just figured since I have you hear, might as well ask to see if you know of a good one, or just some good keywords to search with. And I dont exactly have a "ton" of money, so is agar exspensive? As for the SAB something that isn't too expensive would be preferred.

Edit: To all of those that have helped me in this grow, as soon as I get to the 50 post requirement, 5 mushies will be headed you way! Thank you!


Edited by dark_nemesis (10/16/13 10:31 PM)


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
Trusted Cultivator
Re: Round 2 [Re: dark_nemesis]
    #18988879 - 10/16/13 10:56 PM (10 years, 3 months ago)

Not contams. But, Still Air Box is the way to go with prints. I use a Tupperware container when making prints. I leave the lid on, but not sealed. The small amount of air current helps to dry the cap just a bit while it deposits the spores. I did want to mention that Agar is the most interesting way to understand not only your sterile techniques, but also how and what you are working with.

  If you decide to get into agar, Frank Horrigan/ Roger Rabbit (videos are worth $9.00) or Stro all have great information about the beginning to end process of Agar.

Essentially you can watch your process in action. Clone and then isolate the sectors. I would definitely study up on Grain's and proper Grain techniques if you are planning on moving to Agar.


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Offlinedark_nemesis
The Initiate
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Registered: 09/07/13
Posts: 83
Last seen: 10 months, 8 days
Re: Round 2 [Re: Sockadin]
    #18988894 - 10/16/13 10:59 PM (10 years, 3 months ago)

Ok thank you again for the information. Has anyone tried this tek?

http://www.shroomery.org/forums/showflat.php/Number/16044347/fpart/1/vc/1

Saw it and it seems pretty interesting and seems like it would remove the need for a SAB for the most part?


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