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OfflineJahLambsbread
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Registered: 08/08/10
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cloning question
    #18908379 - 09/29/13 06:46 PM (10 years, 4 months ago)

when cloning, can u just drop the tissue straight into a half pint jar with the vermiculite and everything pre-made so u don't have to transfer it later?


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OfflineStromriderM
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Re: cloning question [Re: JahLambsbread]
    #18908389 - 09/29/13 06:48 PM (10 years, 4 months ago)

You'd have to be the luckiest person on the planet for that to work


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InvisibleWilly Wonka
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Re: cloning question [Re: Stromrider]
    #18908586 - 09/29/13 07:28 PM (10 years, 4 months ago)

No you have to drop the tissue onto agar.  Then the culture might still sector out.  At which point you will need to transfer each sector to a new agar dish until you have a mono culture.  Once you have a mono culture you will need to fruit it to test it.  You need to test it to see if you have isolated the strain with the qualities of your original fruit. 


--------------------
AMU



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OfflineJahLambsbread
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Re: cloning question [Re: Willy Wonka]
    #18908885 - 09/29/13 08:42 PM (10 years, 4 months ago)

i don't have agrcan i just put it in a plastic bag?


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OfflinePussyFart
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Re: cloning question [Re: Willy Wonka]
    #18909696 - 09/30/13 01:08 AM (10 years, 4 months ago)

Quote:

Willy Wonka said:
No you have to drop the tissue onto agar.  Then the culture might still sector out.  At which point you will need to transfer each sector to a new agar dish until you have a mono culture.  Once you have a mono culture you will need to fruit it to test it.  You need to test it to see if you have isolated the strain with the qualities of your original fruit.



That would be isolating, not cloning.

Isolation is not needed for cloning.

If it is a clean tissue sample it would work.

We use agar dishes to make sure the culture is clean.


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