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OfflineSexySmurff
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Pan Goliath Mystery Contam on Agar
    #18907769 - 09/29/13 04:23 PM (10 years, 3 months ago)

long story short.  2 pan goliaths from a sponsor and 2 replacements, so in all 4 syringes and 30 plates.  i am currently growing out 2 other pans and 5 others cubes so in my opinion the batch that these syringes came from is is definitely contaminated.

but by what. 



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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


Edited by SexySmurff (10/01/13 09:47 AM)


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OfflineSexySmurff
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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18907860 - 09/29/13 04:48 PM (10 years, 3 months ago)

i have already been able to move the pan sandoze and pan hawiians onto grain.







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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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OfflineCircle K
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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18908170 - 09/29/13 06:06 PM (10 years, 3 months ago)

Quote:

SexySmurff said:
i have already been able to move the pan sandoze and pan hawiians onto grain.




I've got no idea what your contams are, but those jars look so awesome. Did you spawn with agar, or however that's said? How long have those jars been going for?


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Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflineDorfnob
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Re: Any clues as to what contam this is? [Re: Circle K]
    #18908203 - 09/29/13 06:12 PM (10 years, 3 months ago)

microdochium nivale I'm guessin but I'm not totally sure, jars look nice good job :thumbup:


Edited by Dorfnob (09/29/13 06:16 PM)


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OfflineSexySmurff
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Re: Any clues as to what contam this is? [Re: Circle K]
    #18908605 - 09/29/13 07:30 PM (10 years, 3 months ago)

those jars were spawned with agar on 9/24.

grain is wheat. i used to use milo but having switched to wheat i feel like wtf have i been doing all this time. 

milo is such a pita to hydrate without splitting. 

wheat is so much easier.

and i do not shake following inoculation with agar.  per rr's suggestion, i always let the agar jump off into the grain and around day 5.  most are done around day 11.


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Re: Any clues as to what contam this is? [Re: Dorfnob]
    #18913862 - 09/30/13 11:26 PM (10 years, 3 months ago)

Quote:

Dorfnob said:
microdochium nivale I'm guessin but I'm not totally sure, jars look nice good job :thumbup:





could very well be.  after looking at macro colonies i notice that they tend to form concentric circles. 

here is what they look like as of todaY.



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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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OfflineSexySmurff
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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18919532 - 10/02/13 03:15 AM (10 years, 3 months ago)

further progression of the contaminant





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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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OfflineDorfnob
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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18931837 - 10/04/13 03:49 PM (10 years, 3 months ago)

Do you have any other ideas for what it could be And why are you keeping it around? I think once I run out of the grain I havE now I'll give wheat a try.


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Re: Any clues as to what contam this is? [Re: Dorfnob]
    #18948300 - 10/08/13 06:10 AM (10 years, 3 months ago)

Definitely, on the wheat. You'll never look back.  As far as keeping them around, I still want to identify them so I am letting ut grow out


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18976608 - 10/14/13 01:25 PM (10 years, 3 months ago)

it's pretty much confirmed that this batch was contamed right out of the gate with several members messaging me confirming that they too got the same contam from same batch / same vendor. 

so having turned to another sponsor for this particular strain, let's see what happens.


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18978032 - 10/14/13 07:01 PM (10 years, 3 months ago)

so what is the probability that the same contam would show up in five different syringes obtained from two different sponsors????








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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18983245 - 10/15/13 08:30 PM (10 years, 3 months ago)

That's a bummer man sorry to hear your having such bad luck lately.


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Re: Any clues as to what contam this is? [Re: Dorfnob]
    #18983558 - 10/15/13 09:39 PM (10 years, 3 months ago)

bad luck with trying to obtain a nice clean pan cambo syringe. 


:wink:


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18996732 - 10/18/13 06:08 PM (10 years, 3 months ago)

so i was told by the second sponsor vendor that pans aren't meant for agar but dung based substrates.  hmmmmmmm. 

these are the results of the syringe obtained from second source but exhibiting the same contam as the primary source. 

 


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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OfflineMAIA
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Re: Any clues as to what contam this is? [Re: SexySmurff]
    #18997038 - 10/18/13 07:24 PM (10 years, 3 months ago)

Quote:

SexySmurff said:
so i was told by the second sponsor vendor that pans aren't meant for agar but dung based substrates.  hmmmmmmm. 





That's not a very objective answer ... at all ! In mycology, agar is supposed to be an universal medium.


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OfflineSexySmurff
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Re: Any clues as to what contam this is? [Re: MAIA]
    #18997111 - 10/18/13 07:39 PM (10 years, 3 months ago)

my thoughts exactly!


--------------------
Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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