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slartibartfast
Stranger



Registered: 05/13/08
Posts: 203
Last seen: 4 years, 9 days
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Spawn Bag issues
#18904984 - 09/28/13 10:54 PM (10 years, 4 months ago) |
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Greetings all, I am at a bit of a loss to explain my continued failure with spawn bags and would appreciate any suggestions. I have been getting poor (ok almost a complete loss) results in my efforts to use spawn bags to produce lots of cube spawn so i will explain what i do and see if anyone can figure out whats going wrong. I prepare the grain in the same way i do for spawn production in 1 or 2 quart jars and have experimented with final moisture contents between 40-55% and then PC the bags (2-4 quarts of grain per bag) for 3 hrs and then allow to cool in front of my flow hood overnight and inoculate with either a slurry syringe (20cc) or by grain to grain and then impulse seal the bags (filter patch bags from FP) and incubate at a temperature in the low 70's and almost without fail after a few days of colonization they go green or slimy. I have tried different cube strains (PE, Burma, Wollongong) with the same results. When i use Jars of 1 or 2 quarts volume i have no problems and when i use the same bags and same prep for oysters or hericeum i have no problems so i can't see it being either a preparation or problem of bad technique so I am At a loss to figure out why the cubes go wrong all the time. Any help is appreciated Slart
-------------------- Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit. Trades welcome
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kinkaku
I AM THE LAW!!!!




Registered: 04/02/13
Posts: 1,322
Loc: Россия
Last seen: 8 years, 1 month
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first off those arent different cube strains they are varieties(cube is a cube)
second do you protect the filter patches in any way when autoclaving? I had the same problem due to comprimised filter patches, so I would inspect them before use. you could also try increasing the pc times.
oh and oysters have a hardcore mycelium and are good at beating contams to the punch so thats not a good comparison.
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slartibartfast
Stranger



Registered: 05/13/08
Posts: 203
Last seen: 4 years, 9 days
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Re: Spawn Bag issues [Re: kinkaku]
#18905279 - 09/29/13 12:31 AM (10 years, 4 months ago) |
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Thanks for the response and i have always thought strain and variety to be much the same (in horticulture they are used interchangeably so the term cultivar is preferred) but nomenclatural accuracy aside No i don't protect the filter patches while PC'ing so will give that a try. And while oysters will rip through a substrate super fast Hericium is slower in my experience than any Cubes i have grown so i would have thought the bags where coming through the PC'ing in good condition. Slart
-------------------- Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit. Trades welcome
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Zeparthian
Young Mycologist


Registered: 10/01/12
Posts: 223
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Not sure if this is applying to you, but if you just can't figure it out maybe PC was the mistake.
I moved away from sea level, now im well above 7,000 feet for sure. I had to increase my PC times because I was getting only 33% success rate due to you must increase PC times by 15 minutes every thousand feet past 4,000. Someone could have misinformed me on this one, but i know my success rate is back up to near 100%  Good luck mate.
-------------------- Having troubles? RR's here to help! Let's Grow Mushrooms - RR Watch, absorb, and put that information to use! With a little help, miracles do happen
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