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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886342 - 09/24/13 07:23 PM (10 years, 4 months ago)

Idk how you would g2g to a PF jar unless it didn't have a verm barrier and then it would just be a brf cake in a half pint with a grain lid. Some of them look bacterial but like I said I'm not expert and have only seen a couple dozen of my own grain jars but none have ever looked like that.


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886360 - 09/24/13 07:26 PM (10 years, 4 months ago)

What kind of grain are you using, if you don't mind me asking? And what strains are you using?


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886377 - 09/24/13 07:30 PM (10 years, 4 months ago)

Strain doesn't matter in the slightest the strain is P.Cubenis but to not be a dick I've used GT B+ Creeper AA+ and they all act exactly the same with MS inoculations aside from AA+ maybe being a little bit slow on average to colonize.

http://www.shroomery.org/forums/showflat.php/Number/18836421#18836421
There's a lot of links in that post about strain discussion by RR and other TC's

I always use organic rye.

On second look up close the top middle and bottom right are the only ones that look suspect the others could all be ok for all I know but I've never seen growth look quite like that on grains but you did shake them right away too.


Edited by Trusted cuItivator (09/24/13 07:35 PM)


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886520 - 09/24/13 08:09 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
Strain doesn't matter in the slightest the strain is P.Cubenis but to not be a dick I've used GT B+ Creeper AA+ and they all act exactly the same with MS inoculations aside from AA+ maybe being a little bit slow on average to colonize.

http://www.shroomery.org/forums/showflat.php/Number/18836421#18836421
There's a lot of links in that post about strain discussion by RR and other TC's

I always use organic rye.

On second look up close the top middle and bottom right are the only ones that look suspect the others could all be ok for all I know but I've never seen growth look quite like that on grains but you did shake them right away too.




Thanks, jefe. I cross posted my pics over in the contam forum. The one red light is one jar (Not pictured) Had a white blob half the size of a thumbprint, above the substrate, growing off of a single seed stuck to a side, which I really can't be sure about.

I appreciate the help, really. As soon as I've got colonization, I'm planning on spawning around half to some trays I picked up at the good ol' mercado. I lost track of my Taz and Hillbilly strains in whichever jar, so that's definitely going to be interesting, when they begin to fruit!


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisiblebodhisattaMDiscordReddit
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18886550 - 09/24/13 08:16 PM (10 years, 4 months ago)

Quote:

Thanks, jefe. I cross posted my pics over in the contam forum. The one red light is one jar (Not pictured) Had a white blob half the size of a thumbprint, above the substrate, growing off of a single seed stuck to a side, which I really can't be sure about.




That always happened to me the first time I made grain jars and was to impatient and shook them more than the one time at 30% and then again at 100% prior to spawning. Always having to make my own mistakes to learn why people say the things they do for a reason.


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Re: WBS Jars inoculated, now for the meantime.. [Re: bodhisatta]
    #18886684 - 09/24/13 08:48 PM (10 years, 4 months ago)

Look good to me, but I am usually wrong anyway so that doesn't mean much


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Sockadin]
    #18887457 - 09/25/13 12:10 AM (10 years, 4 months ago)

Thanks, helps out a little.

I shook each jar around 30 mins back, so now it's pretty much a waiting game. Some clumps were pretty tough to bash off the walls, but I gave each a really good shake. It seems I filled a few of them a little too much, which made shaking kind of a hassle, but instead of pouring a bit out, I kept it safe and shook with each jar closed, filters still on.

I'm gonna check on them periodically, and hopefully sometime this weekend I'll see some colonization, and I'll be sure to snap a few pics for you guys. I'm picking some more supplies up pretty soon, and I'm thinking about using the last cc of each of my two syringes to PF tek-style noc up two extra jars with it, and I'll have those isolated from the rest. Here's hoping this starts looking good!


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSteyner
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18887756 - 09/25/13 03:15 AM (10 years, 4 months ago)

they look great, congrats man. in two days' time they will look near fully colonized, but give it another week. use the best one (make sure no contams and colonized 100%) to make grain liquid culture!

what you do is boil some water, and draw it into an empty syringe with needle whilst boiling. empty the syringe wherever just not back in the pot, and repeat about 4 or 5 times. on the last draw do not empty the syringe, but place the cap on (the cap should be in the boiling water as well to sterilize) and put it in the fridge to cool down. Let it cool properly.

now for the fun part. you want to shake your jar, then flame sterilize the syringe needle and inject the now sterilized and cooled water into the jar through the polyfill/tyvek. keep the syringe in the jar and give it a nice shake to get the loose mycelium into the water. then suck out as much water as possible, remove the syringe and tape up the hole you just made if through the tyvek. Voila, mycelium laden water in your syringe, as good as, no even better than a spore syringe. you won't fill it all the way as a lot of the water will be sucked up by the grains but don't worry, a little goes a long way.

you can use this syringe to noc a jar of sterilized water with one of your special lids and a piece of broken glass/shotglass inside. the myc should grow within a week and the solution will be super clear as you don't need any sugars in it since the water picked up nutrients when shaken through the grains. so contams are easy to spot. from here you can get as many syringes as you need, full of active already germinated and already growing mycelium! the glass in the jar is used to break up the clouds of mycelium to make it easier to suck into your syringes whenever you need to draw from it.

use what's left of the syringe in more grain jars, hopefully by then you have the pc to sterilize things properly! if the syringe is left out for about a week the myc water may get very thick, and at some point maybe after 3 to 4 weeks it will clog the needle so before that, you want to move it to the fridge where it will last for months. same with the liquid culture jar once you see it's colonized far enough.

looking forward to see your pic of that beautiful clear jar with white clouds of mycelium growing inside!


Edited by Steyner (09/25/13 03:43 PM)


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Steyner]
    #18889238 - 09/25/13 01:11 PM (10 years, 4 months ago)

Thanks! I was planning on making liquid cultures with the remaining 1cc in each of my syringes, but the idea for getting myc straight from the jars never occurred to me!

Some of the myc is actually poking through to the face of my jars, and I noc'd them up at around 10 last night. It's nothing big, I'll snap some pics if I get the chance (Not at my pad now), but I'd measure it as a fifth of a centimeter-sized growths that are poking through. I'm hoping they'll look good after a few days!

I went out this morning and stocked up on cleaning supplies, and picked up a 16oz bottle of clear Karo with Vanilla, and I'm boiling up some LC as I type this. I'm gonna let it cool throughout the day, and use up my jars tonight to 'noc up each jar. As soon as those go good, I'm gonna start transferring them to more and more jars.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSmeagol
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18889515 - 09/25/13 02:08 PM (10 years, 4 months ago)

There's a good thread about the dangers of LC by FrankHarrigan. Grain to grain transfers can expand your spawn supply by  10. 1 agar dish becomes 4 quarts, which can becoe 40 quarts.


--------------------
The road to hell is paved with good intentions, but it is not paved with clarity.” -Terence

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InvisibleSkinty
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18889694 - 09/25/13 02:59 PM (10 years, 4 months ago)

Quote:

Circle K said:The scientific part of my brain




This was when I knew this had to be trolling as I knew dog would have removed this with a .44 by now :lol:


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Skinty]
    #18896841 - 09/27/13 12:13 AM (10 years, 4 months ago)

Quote:

Skinty said:
Quote:

Circle K said:The scientific part of my brain




This was when I knew this had to be trolling as I knew dog would have removed this with a .44 by now :lol:




He actually tried, the other week. We convinced him his gun didn't have any bullets in it, so he shot the TV instead.

Anyway, the jars are really coming along. I'm gonna give them another solid week or two, but the mycelium's just shot out, now that I've shaken them! My liquid culture jars are displaying tiny, tiny little spore/myc particles when I swill the jars, so it's gonna be neat seeing those strand together in a few days!

The awesome thing about liquid culture, I've been reading, is that so long as you either have spores or a healthy substrate, you can always use that method, even without a pressure cooker, to conserve spores in that fashion. It could be that I'm all kinds of stoned now, but the ability to even get healthy myc off of a dried mushroom is just all kinds of awesome.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18903854 - 09/28/13 06:17 PM (10 years, 4 months ago)

My jars have taken off, for sure. I've got a good amount of pics of the mycelium in them, along with my two liquid culture jars. There's pretty much nothing but a few near-microscopic bits swirling about in the jars, but figuring I noc'd them each up with just 1CC, I figure it'll take a little while to notice any activity.

Anyway, onto the pictures I've snapped. I'm double sure and run mine through an EXIF scanner just to be safe, but most of them look great.

Here's the lineup. You can easily tell which one is doing the best, but it's got me a bit worried.


I've got no clue what this is, and I'm hoping it's something benign, can anyone diagnose it?


Sans that brown shit, these three best jars are my pride and joy of this entire process.


I snapped a few more, each of a different jar.





I'm gonna include pics of my liquid cultures, each in a pint jar with 1 1/2 tsp of Karo light;



I shake my jars once or twice a day to encourage growth, should I be shaking them more/less? I read that you can make a pint of LC in two days with a magnetic stirrer, but I didn't stick an agitator in either jar. Should I give that a shot, at some point?

Let me know how I'm doing, you guys, this is starting to become awesome. I'm gonna make a few more syringes off of the jars before I put them into trays/tubs (We don't need cakes, where we're going.).

I feel like this was a really close save, if at least half of what I have now goes right. Whatever keeps me from needing to buy more spores tells me that I've done something right.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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InvisibleSkinty
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904456 - 09/28/13 08:41 PM (10 years, 4 months ago)

Wat does dog think :shrug:


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InvisibleChaos Creations
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Re: WBS Jars inoculated, now for the meantime.. [Re: Skinty]
    #18904604 - 09/28/13 09:18 PM (10 years, 4 months ago)

Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Chaos Creations]
    #18904837 - 09/28/13 10:17 PM (10 years, 4 months ago)

Quote:

Skinty said:
Wat does dog think :shrug:




You wouldn't believe it. Every question we ask him, he answers "Yes". That's it. He's either doing that or yelling at us to "Make the shrooms" already. He tried to send me to get him some more bullets, but I slashed my own tires.

Quote:

Chaos Creations said:
Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth




Alright, I'll hold off of that, and work with the next batch I'm able to PC. I've shaken after inoculation, then when my jars were at
20%. Now that they've really taken off, I'm thinking I'll shake some of the lesser performers, that are still quite performing, hah.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePirax
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904876 - 09/28/13 10:27 PM (10 years, 4 months ago)

Those are all screwed
You did almost everything right except the pressure cooker part.
I went to goodwill and found a pressure cooker for like $9. Then ordered the 15psi weight for the lid online for like $6.
Do everything you did again, except do it right, with a pressure cooker. And do some more reading.
I would just use those jars for an outdoor patch. And redo everything the right way with a PC, then G2G, then spawn to bulk/make LC, agar, etc.


Edited by Pirax (09/28/13 10:30 PM)


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OfflineCircle K
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Re: WBS Jars inoculated, now for the meantime.. [Re: Pirax]
    #18904881 - 09/28/13 10:28 PM (10 years, 4 months ago)

Quote:

Pirax said:
Those are all screwed
You did almost everything right except the pressure cooker part.
I went to goodwill and found a pressure cooker for like $9. Then ordered the 15psi weight for the lid online for like $6.
Do everything you did again, except do it right, with a pressure cooker. And do some more reading.





Damn, why am I getting all the downers? I'd say things have started looking really good.


--------------------
Hold on tight, to your dream!

Unless you have shoeboxes, then hold onto those.

Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.



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OfflinePirax
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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18904888 - 09/28/13 10:32 PM (10 years, 4 months ago)

All of those jars are obviously contaminated. You can visibly see bacteria growing, and possibly mold.
Read the edit I made on my original post, you might be able to make an outdoor patch with those but I would not attempt to fruit those inside your house.


Edited by Pirax (09/28/13 10:32 PM)


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Re: WBS Jars inoculated, now for the meantime.. [Re: Circle K]
    #18909095 - 09/29/13 09:24 PM (10 years, 4 months ago)

Quote:

Circle K said:
Quote:

Skinty said:
Wat does dog think :shrug:




You wouldn't believe it. Every question we ask him, he answers "Yes". That's it. He's either doing that or yelling at us to "Make the shrooms" already. He tried to send me to get him some more bullets, but I slashed my own tires.

Quote:

Chaos Creations said:
Honestly I wouldn't make any Grain LCs off of a non PCed jar. The odds just aren't in your favor. Shake less. Id shake maybe 2 times once at 30% colonization and once at maybe 60%. Shaking too much actually damages them and slows growth




Alright, I'll hold off of that, and work with the next batch I'm able to PC. I've shaken after inoculation, then when my jars were at
20%. Now that they've really taken off, I'm thinking I'll shake some of the lesser performers, that are still quite performing, hah.





Don't shake after inoculation. Tell your dog to stop humping the jars there is a little red rocket mark on one of them.


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