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Offlinemathieu
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Clarification on GLC? floating white island 2wks after
    #18903766 - 09/28/13 05:52 PM (10 years, 4 months ago)

I'd appreciate whatever knowledge bombs you guys can drop on me.

This is my first go with GLC... I noc'd up 4x popcorn jars with B+ via MS awhile back, and extracted a few rounds of GLC from them. **It's worth considering that this has been a very non-rhizo myc from the start**

The pictures below are of GLC I extracted from 2 jars 2 weeks ago. I shook pretty violently to get the best myc off of the grains-- anticipating that the EZ-Felt filter patch WAS GOING to get wet, I applied bleach to the filter an hour prior to extraction and put it into my glove box. (I'm moving to injection port / whatman lids for future grain jars intended for GLC)

  • 2 weeks ago - GLC extracted from pure happy white colonized popcorn
  • 1 week ago - 1/2 of this GLC jar was used to noc
    ** I noc'd up 15x 2C plastics, drawing from the GLC jar 2x using the same syringe (re-sterilized between GLC jar draws via boiling water in/out 3x, bleach, alcohol, then flaming).
  • This morning - pure white island apparent on surface of the GLC; when the GLC is allowed to settle, there are threads hanging down that are indiscernible from the myc which sinks to the bottom.




PLEASE READ BEFORE COMMENTING ON THE 2ND IMAGE:
The first round I didn't shake as thoroughly, which left the best myc still attached to the popcorn. The morning after extracting, I was curious to see what came of the extra water injected into the jar, with no intent to use it-- so I poured the excess into a NON-STERILE JAR. When it developed similar bright-white islands, I assumed that it was due to being in a non-sterile environment and kept it around as a reminder and curiosity. It's been in there for about 4 weeks at room temp, and doesn't smell like the myc I know and love, but then I've never smelled a good GLC, and I'm not about to open up my good (?) one and stick my nose in it.



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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18903786 - 09/28/13 05:59 PM (10 years, 4 months ago)

Some wisdom from TL, but I'd really be more comfy with an image to go along with it.
#15540378

Quote:

TranscendingLife said:
Mycelium growing on top of the LC is not a sign of a contam.
I've had several LCs that grew on the top & none were contaminated.
Think of the LC somewhat like an agar dish.
The mycelium will colonize the top of the LC to find some GE & food.

The only bad LC I've had, was due to a bacteria contamination in the GLC jar that I didn't see.




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Edited by mathieu (09/28/13 05:59 PM)


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Invisibleabltsandwich
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18904850 - 09/28/13 10:21 PM (10 years, 4 months ago)

Looks fucked to me. Try it on a jar or cake if you really want to be sure, only way to know.


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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: abltsandwich]
    #18905105 - 09/28/13 11:34 PM (10 years, 4 months ago)

Quote:

abltsandwich said:
Looks fucked to me. Try it on a jar or cake if you really want to be sure, only way to know.




I tried it on 15x PF+ cakes in plastic prior to the wee-little white island forming... :crazy:
Nothing out of the ordinary so far, but all of the myc that took hold from the initial MS innoc and subsequent GLC noc ups have been altogether non-rhizo to start with. My concern is that the possible contam is bright, pristine white and visibly myc-ish.


I'm putting this guy in the fridge so that any negative growth slows until I've gotten a final answer.

Does anyone else have experience with this? The myc-ish roots leading off the bottom of the mini island, plus TL's wisdom from an old thread (above) are leading me to think that it *may* be myc, (first pic ONLY, no plans to move forward w/ the 2nd jar only 1/5 full and coated).



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Invisibleabltsandwich
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18908396 - 09/29/13 06:50 PM (10 years, 4 months ago)

Every time you use an LC you're potentially introducing contamination so the more you use it the more of a chance for contams.


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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: abltsandwich]
    #18909733 - 09/30/13 01:28 AM (10 years, 4 months ago)

Quote:

abltsandwich said:
Every time you use an LC you're potentially introducing contamination so the more you use it the more of a chance for contams.




ik ik...
1st GLC extraction was from spawn jar #A, this GLC was from spawn jar B. I used 1/2 of it last weekend, drawn through an injection port with a *clean* needle 2x. (sucked up bleach, let sit, expunged and sucked up boiling water before alcohol and flaming.

If the GLC is tainted, my gut is telling me that it's from the spawn / filter patch getting wet during extraction process, despite my pre-cleaning it with bleach.


That said, the fact that it has undiscernibly myc-like grown coming down from it, coupled with a few people describing myc on the surface of a GLC, I think it's possible that's what it is; just need some better evidence.


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Edited by mathieu (09/30/13 01:30 AM)


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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18909748 - 09/30/13 01:41 AM (10 years, 4 months ago)

It's worth noting that after this long (2 weeks) the similar growth on the surface of the other (dirty, non-sterile) GLC jar is still bright white 3 weeks later. I'm not very familiar with molds growing on liquid, but wouldn't it have sporulated by now, and likely to another color? (black/forest green/yellowish/...)

Please keep in mind that this liquid has always been myc dense, and in only ~1cm of water the myc has always been pressed up against the surface.

(REPEAT: I NEVER PLANNED TO DO ANYTHING WITH THIS OTHER THAN SEEING HOW MUCH WATER WAS LEFT AFTER EXTRACTION.)




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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18918884 - 10/01/13 11:02 PM (10 years, 4 months ago)

HALP!
Update: I just smelled one of the 15x cakes I knocked up with this (prior to the white island forming)

It has a DEEP myc smell.  it doesn't have the light earthy smell I was hoping for, seems a wee bit stronger than usual; may be due to CO2 balance. --  nor the foul feminist foot smell of a strait up dead cake. most of these 15 plastics reached 90% "colonization" (I hope) of a 2C cake in 8 days without GE. (used 10cc's of LC all over). Either way these guys are going in the fruiting chamber, but I'm seeing if I should hold off on innocing another 15x off what's left in the jar (now with a white island to keep it company).

Any assistance to help me identify the white island would be massively appreciated-- I've seen anecdotal evidence of floating myc, but no proof.

Thanks everyone for your questions and assistant.
It's time to turn on the rabbit signal!



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Offlinemathieu
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18936233 - 10/05/13 02:01 PM (10 years, 4 months ago)

HELP!


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InvisibleNimpo
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Re: Clarification on GLC? floating white island 2wks after [Re: mathieu]
    #18936309 - 10/05/13 02:17 PM (10 years, 4 months ago)

OP just grow em out and then youll know what is what.

Ive made LC before with floating mycelium clumps. They even had some black shit in it.

Im fruiting three monotubs now made with the LC's from that batch. I thought it was clearly contam'd, but the syringes were perfect.


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