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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlineretaardvark
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Registered: 12/22/12
Posts: 64
Last seen: 4 years, 8 months
Lid Sterility and Significance in Shaking
    #18902315 - 09/28/13 11:01 AM (10 years, 4 months ago)

In my microbiology class, it is considered absolutely terrible sterile procedure to invert a culture tube (~100ml) so as to expose sterile media to the inside of the lid. The reason given to me by my professor is that the autoclave cannot effectively sterilize anything with such poor thermal conductivity -- the portion of the tube with no media. However, the tubes are only about one quarter full.

Is this principle significant with the use of quart jars for grain spawn?
Or does the relatively large volume of grain in the jar (~75% full) conduct heat/pressure through the jars much more than, say, a quarter-full culture tube?


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Lid Sterility and Significance in Shaking [Re: retaardvark]
    #18902392 - 09/28/13 11:20 AM (10 years, 4 months ago)

Quote:

retaardvark said:
In my microbiology class, it is considered absolutely terrible sterile procedure to invert a culture tube (~100ml) so as to expose sterile media to the inside of the lid. The reason given to me by my professor is that the autoclave cannot effectively sterilize anything with such poor thermal conductivity -- the portion of the tube with no media. However, the tubes are only about one quarter full.

Is this principle significant with the use of quart jars for grain spawn?
Or does the relatively large volume of grain in the jar (~75% full) conduct heat/pressure through the jars much more than, say, a quarter-full culture tube?





Eventually everything in a PC/Autoclave will reach 250f +.  This has nothing to do with thermal conductivity.  I think something was lost in translation there.  I find it hard to believe that we would be using lids on culture tubes that we assume are not sterile.

It may have something to do with only running the autoclave 15 minutes.  Yes glass is horrible at conducting heat, but eventually it will still get to temp. 

I run my grain jars and am 100% certain that 100% of the media in my PC is 250F @ 15 psi.  This pretty much kills everything inside of the PC including jar lids.


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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