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Offlineosorus
Unfolding the Stone


Registered: 09/18/12
Posts: 68
Last seen: 10 years, 3 days
PF B+ Journal +HQ Pics
    #18901835 - 09/28/13 08:00 AM (10 years, 4 months ago)

Hello ! Thanks for checking out the thread.

After a handful of past cultivation attempts with varying degrees of success, and with the anticipation for the coming liberty cap season being too much altogether, I have decided to don the lab coat once again. It is my intention to keep a journal of my trials and tribulations over the next few weeks for the purpose of providing entertainment to those who wish to follow, and hopefully we can all learn a little something along the way. I have been fortunate to have the use of a good camera right now and will be providing the best photographs I can as things progress and hopefully dish out some nice fruit porn in a few weeks!

Initially I will be PF Tek'ing 6 B+ cakes made up with the standard BRF and vermiculite. After colonization I am planning to use one or two of the initial cakes to inoculate several more jars of substrate. I have never colonized this way before, only with spores, so there will be a learning experience here for me too.

After successful incubation, the plan is to birth and fruit the remaining cakes as per the standard PF tek. As part of my experimentation, I will be birthing the second round of jars to a bulk substrate, again something I have no experience with.

So, appreciating all advice and criticisms along the way, I hope you'll join me on this journey of discovery over the next few weeks.

Attached are the first pics of jar sterilization and inoculation.
I'm using half pint wide mouth masons with the substrate as described above. Jars were pressure cooked for 80+ minutes to sterilize before being inoculated with the B+ spores. Around 1/2-1cl per inoculation point. 2 points per jar. I have been advised to keep the inoculation holes covered until colonization begins, then remove the tin foil cover as shown. Perhaps someone can confirm this as a best practice. There is a ~1inch vermiculite barrier in each jar which should suffice in terms of incoming contamination. All advice welcomed on this one.

Also pictured is the base chamber of my terrarium with water heater. I used this previously during colonization to raise the ambient temperature, but had a much higher rate of contamination. In this case unless otherwise advised, I'm happy letting it fall by the way side and stick to allowing them to colonize at room temperature. Again I am keen to hear thoughts on this.

Some pics! :evil:







Edited by osorus (09/28/13 08:11 AM)


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