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OfflineMyTiny_Shroom
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Little glass vials--LT storage
    #18899692 - 09/27/13 05:29 PM (10 years, 4 months ago)

So I got these little glass vials that I want to store the live myc for the long term.  Reading here that you can store the little piece of myc in these vials in the frig as long as they have food, air, and the right environment.  I think the article said this tek was developed in the 60s for long term myc storage. 
My question is how long will the myc stay alive?
And do I need to drill a small hole on top of the vial lid for air exchange too?  Any input will be welcomed!


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InvisibleFrankHorrigan
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Re: Little glass vials--LT storage [Re: MyTiny_Shroom]
    #18899742 - 09/27/13 05:42 PM (10 years, 4 months ago)

For proper long term storage, you will want to make culture slants and store them at 40F once they are colonized.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineMyTiny_Shroom
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Re: Little glass vials--LT storage [Re: FrankHorrigan]
    #18901234 - 09/28/13 12:46 AM (10 years, 4 months ago)

"When they are ready to go into storage, I snap the cap down all the way (air-tighT) and put them into plastic baggies again."

Thanks, Frank for the neat infos.  I will use that tek next time.
So the cap has a breathing hole for GE, right?


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InvisibleFrankHorrigan
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Re: Little glass vials--LT storage [Re: MyTiny_Shroom]
    #18902274 - 09/28/13 10:50 AM (10 years, 4 months ago)

Depends on what kind of tubes you use.

Those pictured in my link, they are cultubes. The cap has two snaps- one provides GE and one is completely airtight.

You need GE while colonizing. Once they are ready for storage, then you snap the cap down airtight.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineMyTiny_Shroom
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Re: Little glass vials--LT storage [Re: FrankHorrigan]
    #18903630 - 09/28/13 05:20 PM (10 years, 4 months ago)

So while they are in storage they do not need any GE?  I wonder
if the myc will die if no GE.


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InvisibleFrankHorrigan
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Re: Little glass vials--LT storage [Re: MyTiny_Shroom]
    #18903679 - 09/28/13 05:31 PM (10 years, 4 months ago)

You mean when it's at 40F?

It does not.

I have about 40 different cultures that are stored this way and they are all just as viable as when they went in :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineMyTiny_Shroom
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Re: Little glass vials--LT storage [Re: FrankHorrigan]
    #18904657 - 09/28/13 09:29 PM (10 years, 4 months ago)

I thought it was interesting that even at 40F they did not
suffocate when the lid got shut.  Maybe the myc don't need
fresh air in order to survive inside the tubes.
How long you think they will live in there?


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InvisibleFrankHorrigan
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Re: Little glass vials--LT storage [Re: MyTiny_Shroom]
    #18904707 - 09/28/13 09:43 PM (10 years, 4 months ago)

If you put a piece of wood in there for it to chew on, it will be good for years.

If not, the only thing that you need to do is transfer it once a year or so to new slants, the agar will begin to dry out after a while.

When they are in the fridge, the myc does not need GE, it is not growing, it is essentially "dormant."

Any slant tek you see on here will recommend closing your tubes completely when you place it into the fridge.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineMyTiny_Shroom
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Registered: 08/30/13
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Re: Little glass vials--LT storage [Re: FrankHorrigan]
    #18905540 - 09/29/13 01:57 AM (10 years, 4 months ago)

Ohhh, I see it now.
Thanks much for all the good infos.  I still have a lot
to learn here.  Will try that out soon.


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