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OfflineJahLambsbread
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Registered: 08/08/10
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cloning questions
    #18899622 - 09/27/13 05:15 PM (10 years, 4 months ago)

Some mushrooms are just about to drop spores.  I don't know whether i should make a spore print or make a clone of the tissue.

What do you guys recommend and if I do the tissue cloning how do I go about getting the mycelium into a jar?

One tek says to let the tissue sit in a bag untill myceliam grows but how would I getthat into a half pint jar?


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OfflineBlake_Shroom
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Re: cloning questions [Re: JahLambsbread]
    #18899633 - 09/27/13 05:18 PM (10 years, 4 months ago)

I would just take prints. It sounds like you don't know how to clone, and believe me, if you dont have it down good then you'll have a lot of failure

I tried doing the tek where you insert a syringe into the fruit and then into a LC. I contamn'd so many jars after that. The absolute best and most reliable way is to pick a pin forming in a cluster, and put it to agar:thumbup:


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OfflineJahLambsbread
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Re: cloning questions [Re: JahLambsbread]
    #18899639 - 09/27/13 05:18 PM (10 years, 4 months ago)

can i just drop the tissue straight into a half pint jar with the vermiculite and everything pre-made so I don't have to transfer it later?


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OfflineJamesSpawned
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Re: cloning questions [Re: JahLambsbread]
    #18899650 - 09/27/13 05:20 PM (10 years, 4 months ago)

Both. Set the cap aside to print then pull the stem in half to split it cleanly and clone.


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OfflineJahLambsbread
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Re: cloning questions [Re: JahLambsbread]
    #18899655 - 09/27/13 05:21 PM (10 years, 4 months ago)

yeah obviously i don't know how to clone. that is why i am asking...OBVIOUSLY


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OfflineJahLambsbread
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Re: cloning questions [Re: JahLambsbread]
    #18899661 - 09/27/13 05:22 PM (10 years, 4 months ago)

does it matter if the mushroom is a little dry from the spore print rather than straight from the terrarium?


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OfflineBlake_Shroom
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Re: cloning questions [Re: JahLambsbread]
    #18899664 - 09/27/13 05:22 PM (10 years, 4 months ago)



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OfflineJamesSpawned
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Re: cloning questions [Re: JahLambsbread]
    #18899666 - 09/27/13 05:22 PM (10 years, 4 months ago)

OP do you have a SAB?


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