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spacedcwby
Stranger

Registered: 09/21/13
Posts: 9
Last seen: 10 years, 1 month
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LC Jar ?
#18875878 - 09/22/13 01:16 PM (10 years, 5 months ago) |
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Just pulled my first four jars of light karo and water out of the pc. 2 of them sealed - as in vacuum sealed themselves. The other two will still pop if pushed. They were screwed tight before I lifted them out of the pc. Will those two that didn't vacuum have a higher probability of contams once i noc them?
The color is very nice and light golden. Didn't carm up too bad. First attempt with an lc. Had good luck with ms brf jars in the past but my latest attempt seems to have stalled about 3 weeks into colonizing after 5-10 days of "germination" I guess you would call it. Thought lc was a better bet for the money and the addition of a flea market pc could turn into something a bit more than cakes anyhow. Want to try out the dub tub tek with some lc to wbs to horse poo. Thanks!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Your lids should have some sort of filtered gas exchange port. If there is only a self healing injection port you'll get a vacuum when you try to suck up your LC.
Take a picture of your jars with the lids in view.
Edited by Trusted cuItivator (09/22/13 01:20 PM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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If you plan to use a syringe to inoculate those jars, you might get all your spores sucked in to it.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Mushouse
Mycomancer

Registered: 06/26/06
Posts: 500
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Draw air into a syringe through a flame (sterile air), then use that syringe to equalize pressure.
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Quote:
Mushouse said: Draw air into a syringe through a flame (sterile air), then use that syringe to equalize pressure.
Nah, just stuff an empty syringe with polyfil, and put it through the port.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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While the polyfill syringe will work for equalizing pressure, I would be wary using it to relieve the initial vacuum as it can be quite strong.
That much "suck" just might suck through some funk.
I'd suck air in through a flame and actually over pressurize the jar so you only have to do this step once in a while, not every time.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I would start over and use a lid with a filtered hole.
LC supplies are cheap.
Scratch that. I would ditch the whole project and make agar. But that's not what this thread is about. I digress.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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jimbojones
Stranger
Registered: 09/07/13
Posts: 8
Last seen: 10 years, 3 months
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I would just start over. Make some proper lids with a self-healing inoculation port and filtered gas exchange. All you need is tyvek and rtv silicone.
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Quote:
maddchef said: While the polyfill syringe will work for equalizing pressure, I would be wary using it to relieve the initial vacuum as it can be quite strong.
That much "suck" just might suck through some funk.
I'd suck air in through a flame and actually over pressurize the jar so you only have to do this step once in a while, not every time.
I have a hard time believing any sort of contamination would be able to get through multiple inches polyfil.
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Quote:
maddchef said: While the polyfill syringe will work for equalizing pressure, I would be wary using it to relieve the initial vacuum as it can be quite strong.
That much "suck" just might suck through some funk.
I'd suck air in through a flame and actually over pressurize the jar so you only have to do this step once in a while, not every time.
I have a hard time believing any sort of contamination would be able to get through multiple inches polyfil, but I guess it could.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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It's an lc, and probably his first, better to go on the safe side. Plus there was no mention of a pc so in theory he would stuff the dirty syringe with gunked up hands in a nasty environment.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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spacedcwby
Stranger

Registered: 09/21/13
Posts: 9
Last seen: 10 years, 1 month
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PC'd the jars, yes.
Did have a vacuum in them. Was able to noc up 4 jars with a 10cc syringe. One did get a little greedy but after that it was a breeze.
I understand all the pressurization issues and having to equalize, thanks for the advice though. My real question though was if there isn't a vacuum present after a run in the pc, will the lc be more prone to contams.
The karo didn't carm up too bad, one is really quite clear but the other three have a light yellow tinge to them. Not cloudy at all. They are in a closet in the middle of the house in the dark at about 70f or so. Should I bump up the temps with a heat mat under an inch or so below them? I'm assuming rh has nothing to do with noc'ing up an lc.
Thanks everyone. Having fun with this. I want penis envy galore this time around...
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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You should get the vacuum out and get some air in, that's why a filter is recommended. There needs to be some gas exchange, with a vacuum you can't have a gas exchange. You'd be surprised how much vacuum there is in those jars.
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   The Corbett Report Open Source Intelligence News
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spacedcwby
Stranger

Registered: 09/21/13
Posts: 9
Last seen: 10 years, 1 month
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Re: LC Jar ? [Re: Pestile]
#18882123 - 09/23/13 08:15 PM (10 years, 4 months ago) |
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Right on. Will do. Already seeing some little white fluffs floating about. Happy days are ahead.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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After only a day and a half and I wouldn't think that's myc in there.
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spacedcwby
Stranger

Registered: 09/21/13
Posts: 9
Last seen: 10 years, 1 month
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I was pleasantly surprised myself. Used 3 1/2 pt jars and 1 1/4 pt jar. The 1/4pt is probably almost 20% already. Lots of fuzz! One of the 1/2pts isn't doing much but the other two are coming along nicely. Thinking of waiting another couple of days and getting some of the 1/4pt's fuzz to add to the other jars to speed them along. Is this advisable or should I just start with fresh jars to make more lc?
First time lc, so I couldn't be happier at this point. We'll see how sterile I was. Doesn't appear that there are any contams in the jar.
Prepping wbs jars today. Soaked the stuff overnight. Has about 20% sunflower seed. The co-op does sell straight white millet. Would that be more suitable next time I need more?
As for my sub. Torn between the str8 up hpoo or the 50/50 hpoo/straw. Suggestions appreciated!
Bought four sweater sized tubs the other day. About 10" deep and 12x20" probably. Should do me well for a first run with dub tubs.
This is fooking great. I love watching this myc.
Edited by spacedcwby (09/27/13 01:34 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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I wouldn't screw around with LC's just use the syringe you're only adding a vector for contamination to set in by carelessly using high nutrient solutions. The time it's taking you to fool around with that is longer than it would have took to have the spores just germinate themselves in your jars.
I suggest you edit out the sponsor name since you shouldn't be using there syringes for cultivation. Read the rules before posting
The co-op should have rye berries too. My local co op has organic rye berries for something like 79 or 89 cents a pound and you only need 1 cup of them per quart jar. I suggest rye.
I suggest making your own substrate because by the time you spend the money you could have bought everything the substrate they sell you is made of and then the extra money you can spend on the tools needed to prepare it. Then you'll actually learn something rather than hoping for the best.
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spacedcwby
Stranger

Registered: 09/21/13
Posts: 9
Last seen: 10 years, 1 month
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Quote:
bodhisatta said: Then you'll actually learn something rather than hoping for the best.
very true
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