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Offlinemissy
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Registered: 09/19/13
Posts: 17
Last seen: 9 years, 9 months
First Grow w/ Pics: Contam Concern for 2 Jars. pls help.
    #18898643 - 09/27/13 01:09 PM (10 years, 4 months ago)

Hi, everyone, thanks for taking the time to read this & answer, if you did. I know someone that's a first time grower and has a concern for 2 out of 12 of their BRF PF Tek style, 1/2 pint jars.
 
    This person used: PESA spores
-3 parts verm/1 part BRF/1 part water.
-PC'd 1 hr @ 250'F, 15psi, cooled overnight.
-Inoculated 12 jars(2 holes ea, 1/4cc.) in glove box, inside of sterilized grow tent.
-Flamed needle tip and put alcohol on outer syringe (purchased online) as well, wore mask.
-Left jars to darkness at 76'-80'F, with foil removed from lids in tent.

    My friend put the jars in total darkness, but 4 days later, read on this great forum, that they would benefit from getting a 12/12 light schedule, so lights were added.
    The jars were checked every 3 days or so, growth was looking fine and normal. However, on the 13th day they noticed a bit of strange discoloration in 2 of the jars. Now, this could be nothing, but they thought it better to be safe than sorry.       

    I searched this sight a few hours looking for something similar to this, but was unsuccessful.  So, sorry if this has been discussed before. 
   
    There are no odors readily noticeable (she knows not to sniff it).  Some of the substrate looks different around those spots too, I noticed from the pics.  Please, any opinions would be much appreciated, thanks!

    The first 4 pics are of the healthy sides of the 2 jars in question.  These sides look good.  What concerns her is the brownish/tan/thin-looking spot at bottom right of the circular pattern of mycelium in the lower pics and the strange look the BRF has taken on just around the outer edges of the myc.  The brown/tan spots almost seem to interrupt the growth pattern and they are translucent. 
    Is she looking too closely at normal occurrences or should I tell her to throw it out?

    The first 4 are of the good looking myc, the ones after that are the other sides of the same 2 jars, and are the sides in question:





Thanks, again for any help!


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InvisibleGanzig
It's for the street cred
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Registered: 11/29/06
Posts: 8,206
Loc: Oregon Flag
Re: First Grow w/ Pics: Contam Concern for 2 Jars. pls help. [Re: missy]
    #18908252 - 09/29/13 06:22 PM (10 years, 3 months ago)

Lookin good.


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I must keep reminding myself of this. I must keep reminding myself of this.
I must keep reminding myself of this.


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Offlinemissy
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Registered: 09/19/13
Posts: 17
Last seen: 9 years, 9 months
Re: First Grow w/ Pics: Contam Concern for 2 Jars. pls help. [Re: Ganzig]
    #18922849 - 10/02/13 06:39 PM (10 years, 3 months ago)

Thanks for the reply, Ganzig.  I took a look today and there are these white clusters in over half of my 12 jars! They look contaminated, I think.  What do you think and what is it?



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OfflineKizzle
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Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 36 minutes
Trusted Cultivator
Re: First Grow w/ Pics: Contam Concern for 2 Jars. pls help. [Re: missy]
    #18923312 - 10/02/13 08:41 PM (10 years, 3 months ago)

Unless you have a filter on those you should have a dry verm layer, which I'm guessing you do but it should fill up the top otherwise it can move around and stuff can drop through.

At first look I thought maybe you had some primordia forming but looking closer it looks like Trich may have gotten through your layer in which case those bright white balls of mycelium will probably be turning green soon.


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Offlinemissy
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Registered: 09/19/13
Posts: 17
Last seen: 9 years, 9 months
Re: First Grow w/ Pics: Contam Concern for 2 Jars. pls help. [Re: Kizzle]
    #18924747 - 10/03/13 07:12 AM (10 years, 3 months ago)

Thanks, Kizzle.  After I took them from the PC, I noticed my verm layer wasn't good enough, but I was lazy and thought I'd give them a shot.  4 of them look ok, but I'll just start over.  I'm going to make LC with Karo and honey this time around & try the Plastic Tek along with some mason jars for comparison.  I'm nothing, if not persistent!


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Edited by missy (10/03/13 07:29 AM)


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Offlinemissy
Learning
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Registered: 09/19/13
Posts: 17
Last seen: 9 years, 9 months
Re: First Grow w/ Pics: Contam Concern for 2 Jars. pls help. [Re: missy]
    #19154509 - 11/18/13 02:39 PM (10 years, 2 months ago)

………And my persistence has paid off four times now!:thumb up: Thanks everyone for the replies.


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