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wolf8312
Pennywise


Registered: 10/01/12
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Jar for no pour agar
#18897602 - 09/27/13 07:19 AM (10 years, 4 months ago) |
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Hey! I wanted to get into agar at some point so I bought these glass petri dishes and a tube to sterilize them in!

Thing is though on reflection I'm only really concerned with making up a wedge or two to get away from using spore prints/syringes so more an more I've been leaning towards going with a no pour agar tek to avoid opening the jars and to minimize contamination!
I was thinking using small wide mouth jars like the one below but fitting them out with some syringe filters and injection ports. Mix up a mixture of agar and light malt extract, pour into the jars, PC, and then knock up with a spore syringe right through the port!

Question is does this sound like a good plan?
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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SupaThaRipper
Genetics Hoarder



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Re: Jar for no pour agar [Re: wolf8312]
#18897626 - 09/27/13 07:37 AM (10 years, 4 months ago) |
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I cant find the link but deathryder has a tek similar to this in which i do and results have always been great. Im sure youll be OK
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wolf8312
Pennywise


Registered: 10/01/12
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Thanks bud, do you use injection ports as well?
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
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Re: Jar for no pour agar [Re: wolf8312]
#18897696 - 09/27/13 08:18 AM (10 years, 4 months ago) |
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Yea that jar will work fine. Make a SHIP or use an injection port and a form of filtered GE.
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  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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wolf8312
Pennywise


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Thanks bud I will use the same method as I have in my photo on the left there!
Does this method have a high chance of producing a clean culture first time? I was thinking maybe make up about four jars, transfer a clean wedge to a grain jar and then when its fully colonized go grain to grain. Sound about right?
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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PrinceShroom
Experienced Mofo



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Posts: 395
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Re: Jar for no pour agar [Re: wolf8312]
#18897751 - 09/27/13 08:48 AM (10 years, 4 months ago) |
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Yea depending on ur sterile technique it can reliably give you a clean culture. Wedge to jar to g2g is a very tried tested and true method.
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  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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You'll probably find that you hate using the no-pour any way. I have glass petri dishes and I can see why people still prefer plastic disposables. Let alone using no pour. I tried no pour in the really short 4 pack jars they're like 125ml or something. Talk about a pain in the ass to work with in a glove box.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Jar for no pour agar [Re: bodhisatta]
#18897812 - 09/27/13 09:07 AM (10 years, 4 months ago) |
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If you do no-pour agar in a jar, it's gonna be full of condensation. If you try it in a glass petri, it'll boil over.
Don't cut any corners, especially with agar. Pour your plates for the best results.
Also, you're gonna want backup plates, so that on your next grow you don't have to wait for germination again. You can just take a wedge, get it going on another plate then use that on your grain.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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wolf8312
Pennywise


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Re: Jar for no pour agar [Re: 36fuckin5]
#18897889 - 09/27/13 09:34 AM (10 years, 4 months ago) |
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Well my parade is well and truly rained on! Just ordered the jars too!
I understand that if I am wanting to clean things up and make multiple transfers or get into cloning and such that petri dishes would be the much better option. But will it really be such a bad idea if all I am after is a couple of wedges, for one grain jar?
To anyone who likes no pour:- how do you get round the condensation problems? I'm guessing that condensation leads to a build up of water which could in turn increase the probability of contamination right?
That said I have seen a lot of no pour teks by some respected cultivators which kind of led me down this road in the first place! Cant all be bad news?
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Jar for no pour agar [Re: wolf8312]
#18897907 - 09/27/13 09:39 AM (10 years, 4 months ago) |
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For me the condensation in a no pour jar has always ruined things. If I use a small drop of a MS syringe to get things started the germination happens everywhere on the dish because there is too much water to absorb back into the agar and this makes a pretty useless plate unless it's clean from the start.
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36fuckin5
Alchemycologist


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Re: Jar for no pour agar [Re: wolf8312]
#18897908 - 09/27/13 09:39 AM (10 years, 4 months ago) |
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I've tried every damn no-pour TEK out there, and all of them suck.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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wolf8312
Pennywise


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Re: Jar for no pour agar [Re: 36fuckin5]
#18897942 - 09/27/13 09:49 AM (10 years, 4 months ago) |
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-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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memberjockey
Travelling w/out moving



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Re: Jar for no pour agar [Re: wolf8312] 1
#18897949 - 09/27/13 09:51 AM (10 years, 4 months ago) |
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I turn the jars upside down after they've cooled and if there is still a few drops of condensation when I'm ready to use it I turn the jar upside down crack the lid and drain it out, I've had almost zero contamination with the jars than with the petri dishes but even that has been very low.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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I don't know what your first agar recipe will be but I don't endorse cat food agar. I would skip that one. Good luck with the no pour. Not saying it won't work but you'll probably end up finding out for yourself that it's not worth the hassle compared to just good techniques. I had to try the no pour jars myself though too.
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memberjockey
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Re: Jar for no pour agar [Re: wolf8312]
#18897991 - 09/27/13 10:07 AM (10 years, 4 months ago) |
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Quote:
wolf8312 said: http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981
This one sucks?
ITS GOING TO WORK! 
Pouring it is much quicker than making the jars and inoculating a petri dish is much easier than screwing off the cap but if everything is clean my jars never contaminate & when a petri dish does I just isolate it and lift the top enough to take out a good piece before throwing it out. This plate has the mycelium taking over the contamination I took a couple of wedges from this and they're fine showing no contamination at all. Again only the petri dishes show any contamination none of the jars did, you might keep all your stuff upside down since the contamination usually starts at the edge of the dish.
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wolf8312
Pennywise


Registered: 10/01/12
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Last seen: 10 days, 10 hours
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Thanks dude! Thing is win or lose its not really that big a deal to me! My ambitions are just not in the same league as some of the heavy weight cultivators around here! I just want an introduction to agar really! Your results seem good which is encouraging!
I wasn't even planning on opening the jar though I might crack it like u said to drain the condensation (in a GB!). My plan was to use rubber injection ports and inject the spores into the jar that way! Will also use a syringe filter for GE.
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Quote:
bodhisatta said: For me the condensation in a no pour jar has always ruined things. If I use a small drop of a MS syringe to get things started the germination happens everywhere on the dish because there is too much water to absorb back into the agar and this makes a pretty useless plate unless it's clean from the start.

Quote:
wolf8312 said: http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981
This one sucks?
ITS GOING TO WORK! 
Sucks ass. Condensation all over the place. Most of Doc_T's TEKs aren't too good.
Quote:
memberjockey said: I turn the jars upside down after they've cooled and if there is still a few drops of condensation when I'm ready to use it I turn the jar upside down crack the lid and drain it out, I've had almost zero contamination with the jars than with the petri dishes but even that has been very low.
That's awful procedure. Never turn your plates upside down or open them for longer than absolutely necessary. If you'd use proper technique in the first place, you wouldn't have to fix things later.
Also, that water that runs out is highly nutritious. It can get to the edge of your jar/plate and let contams creep in.
You just can't cut corners with agar at all.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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wolf8312
Pennywise


Registered: 10/01/12
Posts: 2,356
Last seen: 10 days, 10 hours
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Re: Jar for no pour agar [Re: 36fuckin5]
#18898178 - 09/27/13 11:07 AM (10 years, 4 months ago) |
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Hum the voice of experience Vs what I want to believe! 
I'll let you know how it goes!
-------------------- "I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of." Pennywise the dancing clown
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memberjockey
Travelling w/out moving



Registered: 11/07/11
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Re: Jar for no pour agar [Re: 36fuckin5]
#18898289 - 09/27/13 11:35 AM (10 years, 4 months ago) |
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Quote:
PrinceShroom said: Yea depending on ur sterile technique it can reliably give you a clean culture. Wedge to jar to g2g is a very tried tested and true method.

I drained them when I was ready to use them if there was water sitting on the top because if I didn't and just add the spores they water logged them, not on the plates just the jars.
I don't store the plates upside down?
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Quote:
memberjockey said: I don't store the plates upside down?
Nope. The only thing that'll do is cause contams to come in through the lid, which will be covered in basically LC.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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