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OfflineTheApprentice
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Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 2 months
Re: Monotub woes.... [Re: FrankHorrigan]
    #18896959 - 09/27/13 12:49 AM (10 years, 4 months ago)

Quote:

36fuckin5 said:
Antibacterial soap kills bacteria, not mold. You just learned that bleach doesn't kill mold spores. It kills the mycelium and then gives the spores some water.

Next time use alcohol.





Very good info!

I checked my alcohol bottle.  It says ETHEL Alcohol. 

Don't know how I grabbed that by mistake.  That's what I've been using
for g2g and agar work.  hmmm..

Quote:

FrankHorrigan said:
Quote:

TheApprentice said:
Quote:

JamesSpawned said:
How did you inoculate? I find its best to use soap with bleach added to it to get things really clean and lift small particles like spores from the plastic. Also, do you feel you were thorough with the bleach?




I never clean my tubs, even when they contam. I rinse them out and wipe them down and spawn again.

The issue is undoubtedly in your spawn.


It was a bad jar :frown:




Well, I have a question in regards to your statement.

I went to check on some agar trasnsfers I made on 9/25

One of the jars had the same green mold growin profusely.

EVEN ON THE AGAR WEDGE...

My question is,

If my monotub contam came from a jar, then why wasn't I
able to see it like I can see it in the jar?



Also, I had a jar from the same g2g batch that broke on me 75% colonized.

I picked the colonized pieces up off the floor, put them in a tray
DUNKED THEM

Then fruited the tray using the Bubble Wrap TEK.
Straight WBS

Like I said, from the same g2g batch.  That jar was supposed
to make it in the tub.

So, WTF?  All the jars couldn't have been bad, which means
the master jar wasn't bad. 

So if the problem with this tub came from a jar,
not a bad cleaning...

HOW CAN I PREVENT THIS NEXT TIME?

WHY COULDN'T I SEE THE CONTAM?

WHY DIDN'T I SMELL IT WHEN I SNIFFED THE JAR?

I'm so confused right now:confused:


--------------------


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Invisible404
error
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Registered: 08/20/10
Posts: 14,539
Re: Monotub woes.... *DELETED* [Re: TheApprentice]
    #18896981 - 09/27/13 12:57 AM (10 years, 4 months ago)

Post deleted by StateOfMind404

Reason for deletion: o0



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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Monotub woes.... [Re: 404]
    #18897074 - 09/27/13 01:28 AM (10 years, 4 months ago)

Because molds don't go green on grains very often.

You need to use proper sterile technique.

Molds that contam your grains after a G2G won't be going green before you spawn to bulk IME. Those are the sneaky little bastards.

Ditch the cultures that are going green. Start fresh. Odds are the mold and mycelium are mingling together so closely they are not able to be easily separated.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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