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CaseyJones199
Live it all for real


Registered: 09/12/12
Posts: 82
Last seen: 10 months, 25 days
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Sterilizing SFDs after use on a contaminated jar
#18894759 - 09/26/13 04:05 PM (10 years, 4 months ago) |
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Lets say someone has a jar that is colonizing with the desired fungi species. It could be a grain jar or one of those no-pour wide-mouth 1/4 pint agar jars that TransendingLife makes. Both of those could use a whole (uncut) SFD as a GE filter.
Now lets say that the jar gets a contamination and the substrate (or media) is fucked. Now we know that the glass jar can be sterilized with several bleach baths and finally a 1 hour cycle in a PC or autoclave, but what are the options with the whole SFD that was used for the jar that became contaminated?
You can't just throw the SFDs in a bleach bath, or could you? And no one wants to throw out an SFD. So would it be a viable option to take a clean quart jar, stuff it with the "contaminated" SFDs, place a filtered lid on the jar and then PC or autoclave it? Would the SFDs be safe to use again or should an SFD that experienced a contam be thrown out?
FYI I am asking specifically about whole, uncut SFDs. Not quarter-sized SFDs that were cut and aren't whole.
TL;DR How would you go about sterilizing an SFD for reuse after that SFD was on a jar that became contaminated.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Sterilizing SFDs after use on a contaminated jar [Re: CaseyJones199]
#18894777 - 09/26/13 04:10 PM (10 years, 4 months ago) |
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Just use it again. No need to clean it. It'll be sterilized in your PC.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Sterilizing SFDs after use on a contaminated jar [Re: 36fuckin5]
#18895226 - 09/26/13 06:13 PM (10 years, 4 months ago) |
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If the PC sterilizes the grains how could it not sterilize the SFD 
This is also why I don't bother going all ape shit crazy about washing jars they only get rinsed after being used, anything that's left over is just more food for my myc.
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