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Offlinefatchillin
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Registered: 10/24/11
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PH of agar effecting germination?
    #18894508 - 09/26/13 03:17 PM (10 years, 4 months ago)

Here's my process + my problem:

-PDA ingredients: 1/6th big package of agar agar (appr. 7 grams), 2 scoops organic potato flakes, 1 teaspoon organic honey, and 500ml water. heated until completely dissolved.
-prepped PDA agar with no PC, only boiling water sterilized for 1.75 hours (while stirring every ~25 minutes)
-poured PDA into dishes in a SAB
-inoculated some dishes with spores, some with wild edible cultures.
-1/20 dishes contaminated post inoculation.
-while only 2/14 used dishes have germinated

All done with super sterile procedure ^

The only growth i have right now is an outdoor woodlover variety, so i was wondering if that strain would be more prone to being able to survive the conditions of the agar plate while other strains (edible / medicinal cultures + 2 cube strains) have yet to germinate.

Would it be possible an ingredient in my potato flakes is hindering the germination of the spores? i feel it may be possible due to the brand / type, could organic potato flakes with seasonings be detrimental for agar plates? (I feel that's where I messed up / cause of bad germination)

Thanks Guys, Much Love


Edited by fatchillin (09/26/13 03:34 PM)


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