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OfflineBeppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 3 months
Golden teacher syringe contams
    #18894218 - 09/26/13 02:11 PM (10 years, 4 months ago)

So I was advised my syringe was an issue by some members here. So I ran an experiment.

I knocked up 6 new jars. 2 with the contaminated golden teacher, 2 with a new golden teacher, and 2 with Ecuador.

Ecuador is already colonizing. All the golden teachers (including the new syringe) are contaming again. So I hit up freespores for replacements and got my fingers crossed. I suppose I should make a liquid culture with the last CC of equador.

Does this all sound right to you guys conceptually? Also how many jars can I knock up from the LC I'll make? (honey tek)


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OfflineJamesSpawned
Fart smeller! Wait...
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Registered: 01/15/13
Posts: 773
Loc: Treasure Coast
Last seen: 7 years, 2 months
Re: Golden teacher syringe contams [Re: Beppe]
    #18894230 - 09/26/13 02:14 PM (10 years, 4 months ago)

G2G the Ecuador, save the last cc for a new master.


--------------------
ARE YOU NOT ENTERTAINED?!



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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Golden teacher syringe contams [Re: JamesSpawned]
    #18894507 - 09/26/13 03:17 PM (10 years, 4 months ago)

Quote:

JamesSpawned said:
G2G the Ecuador, save the last cc for a new master.




This. Don't risk the last of your good spores on LC that may or may not work out.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineBeppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 3 months
Re: Golden teacher syringe contams [Re: Beppe]
    #18894814 - 09/26/13 04:22 PM (10 years, 4 months ago)

G2G from PF tek? Should I just colonize a jar, shake it, then transfer to a nice big ziplock with new steam sterilized BRF sub? I know 100% colonized cakes are pretty contam resistant so a high spawn: low sub ratio should recolonize fast and then I could do a bulk cake tek.

Why is it LCs aren't so reliable? The honey tek seems to work for most posts I have researched. Just curious?

Note: I have no principle, therefore buying a PC or glove box is out of the question.
Also I have 1 new syringe of the golden teacher strain (two have produced a opaque grey dense fungus/mold) so I want to try that before I give up on these needles.

BUT: my 2 Ecuador cakes are already germinated and begun colonization- MY FIRST. so thank everyone here for the knowledge. Your all stars.


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