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OfflineBlake_Shroom
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20 Tub Isolate grow * 4
    #18892903 - 09/26/13 07:36 AM (10 years, 4 months ago)

Well, this is what i've been waiting for. In late august, I had 20 petris of an iso that I put to RGS in qrt jars. 2-3 weeks later (my house is usually 68*) I G2G'd them all to their own individual spawn bag with 5 qrts of RGS. After those colonized, I spawned them to Coir/Verm. I dont know exactly how much, but I would assume around 1 1/3 brick of coir and 4 qrts of verm all pasteurized using my bulk steam pasteurizer in sig.

4 tubs were put into fruiting 3 days ago. They're starting to knot up. Hopefully a beautiful pinset by the end of the weekend. I'm having a little issue of water pooling on the bottom of the tub. Next tubs will have a liner with a small hole drilled in a bottom corner, with tape put over it. That way when it does build up with water, i'll just take the tape off and drain. Notahacker420 had this idea






The 4 tubs were spawned on sunday. There is some really great growth going on there :laugh: should be put into fruiting by sunday



These 5 tubs were made on tuesday. Just as well, some really great growth. Possibly put them into fruiting on sunday as well.




I had some extra jars that didnt look as good as my other jars that were G2G'd into spawn bags, so i made some buckets up :thumbup: I'll be writing a tek here soon



These 2 spawn jars will be made into buckets as well, probably Friday after I pasteurize all my substrate tomorrow



This jar is a clone from a fruit i called "gigantor", hence that is what i named the culture :P I'll G2G this into a spawn bag and fruit it into a mono to see how well it performs



It was cloned from one of the big fruits in that cluster from this tub. I can't remember exactly which one it was, but it weighed 60 grams wet on the first flush



And now to the spawn bags that are almost fully colonized. There's 7 that will be spawned on friday. There is 1 extra that got some bacteria. I stupidly put a blanket over some bags to help speed up colonization, but man oh man these bags produce some HEAT. luckily i took it off after only 1 night so only this bag is fucked. I'll probably spawn it outside, although I really havent had any luck with grain outside (i throw out my spent substrate and get great outdoor fruits). Since it's getting towards fall and i havent had any luck, i'm really not going to put any effort into spawning it outside.




*******************************************************

I'll update about once every 2 days, until shit starts to get real purdy.

By the way, I've grown out this ISO before and it averaged roughly 7oz dried first flush, with the second flush getting 1oz, then toss outside and pull another oz or so :thumbup:


Edited by Blake_Shroom (09/26/13 07:37 AM)


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InvisiblePestile

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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18892920 - 09/26/13 07:44 AM (10 years, 4 months ago)

Interesting. I'm looking forward to seeing the results! :smile:


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Offlineely2121
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Re: 20 Tub Isolate grow [Re: Pestile]
    #18898538 - 09/27/13 12:41 PM (10 years, 4 months ago)

good stuff :smile:
:popcorn:


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: ely2121]
    #18898676 - 09/27/13 01:16 PM (10 years, 4 months ago)






There's some pins popping up. Should be quite the pinset

I'm spawning 7 tubs today and tomorrow, for a total of 16 tubs colonizing (a few already done) and 4 tubs fruiting. And 3 buckets fruiting, 2 more to be spawned this weekend too.


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Offlinenn-IlliniSpiralDMT
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18898898 - 09/27/13 02:12 PM (10 years, 4 months ago)

How many quarts do you use per bag? Is there anyway you could do a how to tek for your mycobags? I would love that! also how do you inoculate them? With agar, lc, g2g, MS etc etc


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18899019 - 09/27/13 02:45 PM (10 years, 4 months ago)



And here's a tub from a week ago. I had 3 tubs going, 1 contam'd right after fruiting, 1 contam'd after pins, and this one went through a full first flush and was tossed so i can cycle tubs. This was the same culture as all of the current tubs, but with 1 key difference.

I had 3 jars colonizing when i went out of town. They weren't good enough to g2g into bags at the time, so i let the colonize more. when I got back, i G2G'd them into bags, then spawned. My culture im working with now is not as "old". Rather than colonize jars, I had 20 petris made up and they sat in a fridge for well over a month, although I can tell this culture is losing its vigor.

I started this strain on agar in late march, maybe early april. I made some transfer, put to grain, and grew out a ton of plates. I took some clones, cleaned up cultures, and here we are. I chose this specific one because it was from the best cluster of the best producing tub, and I have results to prove its quality (averaging 7.5 oz dried first flush). But I took some pieces from my slants, and i can tell i need to find a new culture.

I have some maz on agar now, which i will find iso with from straight transfers, not growing out and cloning.


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: nn-IlliniSpiralDMT]
    #18899020 - 09/27/13 02:46 PM (10 years, 4 months ago)

Quote:

nn-IlliniSpiralDMT said:
How many quarts do you use per bag? Is there anyway you could do a how to tek for your mycobags? I would love that! also how do you inoculate them? With agar, lc, g2g, MS etc etc



I have a tek i wrote in my signature. I only use agar when i inoculate. 5 qrts of spawn per bag, usually half a qrt of spawn when g2g, making for a total of 5 1/2 qrts of spawn per tub


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18910665 - 09/30/13 10:21 AM (10 years, 3 months ago)









Got some pins in my tubs that are in fruiting. Looks pretty good. Gotta  love all those clusters :laugh:

Also, a lot of tubs are fully colonized. I'll put those into fruiting today


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18920984 - 10/02/13 12:57 PM (10 years, 3 months ago)






Well, 2 of them look great! 1 looks pretty decent and the other looks to be struggling. My house is pretty cold so that's why there's not much growth. I'm hoping for some dense meaty fruits :laugh:

I put 9 other tubs into fruiting today. No pins yet, some have knots. Since I dont have a good camera, I'm going to wait until pins to post pics :thumbup:

Out of the other 7 tubs, 5 should be ready for fruiting by friday. The other 2 had some bacteria in the spawn bag. I dunked my grains for a while and added some extra jars into the tubs. I had the sub ready and didnt need these 2 tubs for a while, so i spawned it anyways. Worst comes to worse, the sub will colonize, then contam, then i'll throw it outside. I get way better results putting out a colonized sub outside then spawning an outdoor bed anyways

Here's one of the buckets. The other 2 have yet to knot up yet.



Edited by Blake_Shroom (10/02/13 12:59 PM)


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OfflineStromriderM
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18921444 - 10/02/13 01:50 PM (10 years, 3 months ago)

Damn Blake you've been a busy beaver! Nice work :thumbup:


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Offlinetybojengles
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Re: 20 Tub Isolate grow [Re: Stromrider]
    #18921450 - 10/02/13 01:51 PM (10 years, 3 months ago)

Very nice!  I'm totallyz jelly.


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: tybojengles]
    #18930927 - 10/04/13 12:06 PM (10 years, 3 months ago)



Well my internet if fucking up real good right now, so I can't really upload pics :/

Here's the one I could upload. At least it's the best one :shrug:


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OfflineDeadPhan
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18930946 - 10/04/13 12:12 PM (10 years, 3 months ago)

Quote:

Blake_Shroom said:


Well my internet if fucking up real good right now, so I can't really upload pics :/

Here's the one I could upload. At least it's the best one :shrug:



:justcantwait:


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InvisibleFrankHorrigan
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Re: 20 Tub Isolate grow [Re: DeadPhan]
    #18930971 - 10/04/13 12:18 PM (10 years, 3 months ago)

:popcorn:

Welcome to my world, Blake! :rofl:

I'm glad the isolate turned out good. I personally would not put an untested isolate to 20 tubs, but you picked a good one :wink:


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18931411 - 10/04/13 02:17 PM (10 years, 3 months ago)

Not necessarily untested. I've done like 3 or 4 tubs with this iso. It averaged 6.5-7oz.

By the looks of these 4 tubs, i feel like my average might be lower, maybe at 5.5-6, but we'll see after I harvest this tub and it dries.


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18935683 - 10/05/13 11:19 AM (10 years, 3 months ago)




Harvested the first one. The next one will be harvested tonight or tomorrow.

If you have seen any of my other threads, I let my guys over mature just a bit. I know this, I know its not prime picking time, but its what I like so please keep spore comments or potency comments to yourself




This is one of the 4 tubs fruited at the same time. These guys are growing so slow. Maybe a bacteria issue, i dont know. Regardless, I know they will be dense as hell when I do harvest



I think this one is going to hurt my average, but its all good. Just weird that its the same culture, but I like it being different. There's some monsters in there


Edited by Blake_Shroom (10/05/13 11:48 AM)


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InvisibleSporeo

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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18935994 - 10/05/13 12:57 PM (10 years, 3 months ago)

Sweet pics. Use trash bag liners next time and you won't get all those side pins. :wink: When the substrate starts to evaporate it creates a micro climate between the sub and tub wall. The humidity will be greater on the sides, leading to side pins.


Edited by Sporeo (10/05/13 12:59 PM)


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OfflinePussyFart
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18936050 - 10/05/13 01:14 PM (10 years, 3 months ago)

Quote:

Sporeo said:
When the substrate starts to evaporate consolidate it creates a micro climate between the sub and tub wall.



:thumbup:


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InvisibleSporeo

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Re: 20 Tub Isolate grow [Re: PussyFart]
    #18936086 - 10/05/13 01:26 PM (10 years, 3 months ago)

Haha, true. I meant to say when moisture evaluates from the substrate. Sorry lol


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OfflinePussyFart
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18936264 - 10/05/13 02:07 PM (10 years, 3 months ago)

:facepalm:  That is not what consolidation is or why the substrate shrinks and creates that microclimate.....

You are looking for constant evaporation regardless.


Edited by PussyFart (10/05/13 02:07 PM)


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InvisibleSporeo

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Re: 20 Tub Isolate grow [Re: PussyFart]
    #18936347 - 10/05/13 02:28 PM (10 years, 3 months ago)

Then why does the substrate shrink?  I was agreeing with you when I said true and then made it more clear about what I was saying... I've been in the hobby for a couple years. If you substrate doesn't shrink because it loses mostiure then I feel like a fool. Lol


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18936353 - 10/05/13 02:30 PM (10 years, 3 months ago)

I don't use liners. Personal choice. Works better for me. I don't mind side pins.

This is a log for your enjoyment, I'm not asking for help. I've got my process down and like the way i do it,differs t strokes for differs t folks


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InvisibleSporeo

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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18936383 - 10/05/13 02:39 PM (10 years, 3 months ago)

Okay. Just trying to help. Nice log!


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OfflinePussyFart
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18936397 - 10/05/13 02:42 PM (10 years, 3 months ago)

Quote:

Sporeo said:
Then why does the substrate shrink?  I was agreeing with you when I said true and then made it more clear about what I was saying... I've been in the hobby for a couple years. If you substrate doesn't shrink because it loses mostiure then I feel like a fool. Lol



I mean sure, loss of moisture helps a little, but consolidation is the mycellium digesting the available substrate...

Quote:

RogerRabbit said:
The first thing is full colonization, plus a week or more for the mycelium to 'consolidate' its hold on the substrate, thereby digesting some of the food it has colonized.
RR



http://www.shroomery.org/forums/showflat.php/Number/13356414#13356414

This is why the substrate shrinks....

If it was just from moisture loss, then when we rehydrate it it would expand somewhat to its original size.

At least thats my logic....I could be wrong.


Edited by PussyFart (10/05/13 02:44 PM)


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18936419 - 10/05/13 02:51 PM (10 years, 3 months ago)

Quote:

Sporeo said:
Okay. Just trying to help. Nice log!



Sorry didn't mean to sound like a dick. I just always get shit from letting my tubs over mature.

For me, liners take a bit longer to use. With rgs it sucks because the grains are so small the get behind the liner. Plus my yield is better without a liner


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InvisibleSporeo

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Re: 20 Tub Isolate grow [Re: PussyFart]
    #18939799 - 10/06/13 11:22 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

Sporeo said:
Then why does the substrate shrink?  I was agreeing with you when I said true and then made it more clear about what I was saying... I've been in the hobby for a couple years. If you substrate doesn't shrink because it loses mostiure then I feel like a fool. Lol



I mean sure, loss of moisture helps a little, but consolidation is the mycellium digesting the available substrate...

Quote:

RogerRabbit said:
The first thing is full colonization, plus a week or more for the mycelium to 'consolidate' its hold on the substrate, thereby digesting some of the food it has colonized.
RR



http://www.shroomery.org/forums/showflat.php/Number/13356414#13356414

This is why the substrate shrinks....

If it was just from moisture loss, then when we rehydrate it it would expand somewhat to its original size.

At least thats my logic....I could be wrong.




In my case I dont consolidate. I wait 10-14 days and fruit when I see pins. I see pins 3 days after full colonization. Get 2 flushes and toss. When my tubs fruit the sub still touches the walls. After a few days it pulls away for the sides. Do tubs consolidate after fresh air and fruiting conditions are introduced? Just wondering lol

OP, how do you not benefit from a liner in your tub? That's the first time I've ever heard that. Lol


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InvisibleFrankHorrigan
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Re: 20 Tub Isolate grow [Re: Sporeo]
    #18939840 - 10/06/13 11:35 AM (10 years, 3 months ago)

"Consolidation" is a misleading word here, IMO.

The substrate shrinks as the mycelium digests it. This process begins once the mycelium starts growing. It has nothing to do with how long you keep it under colonizing conditions.

However, moisture loss from evaporation and fruit production will speed this shrinking along.

Also, fruit bulk at 100%.

Quote:

  • Consolidation is for highly nutritious substrates such as BRF + verm. The mycelium needs time to digest the substrate before fruiting.

  • Bulk substrate is less nutritious than PF cakes and can be fruited at 100%. As fresh air is the number one pinning trigger, it is important to give it what it needs as soon as possible for best results.

  • My two cents on the practical application of consolidating bulk is this:

    When I'm fruiting my tubs, I try to avoid aborts whenever possible. I hate the little fuckers, taking up space on my substrate and failing to mature into the beautiful mushroom I know they could be.

    Back when I first started working with tubs a lot, I did consolidate everything. The pinsets could end up being quite massive!

    But come harvest time, I would find a shitload of my pretty pinset aborted. That sucks.

    When colonizing your tub, the RH is near 100%.

    Your mycelium needs this (and high CO2 levels) to grow rapidly while it consumes the available substrate. After 100% colonization, the mycelium realizes it has no more food available to it and starts trying to pin. This process of going from "ready" to "pinning" takes a few days to a week depending on what you are working with.

    Here is my theory:

    By keeping the colonizing conditions (100% RH and high CO2) when your tub is starting to knot up, you are essentially "tricking" the mycelium into thinking that 100% RH and high CO2 levels are what it has to work with.

    When you change this abruptly (at the sight of pins, for example) you are going to be making the conditions less ideal for the pinset that has been produced. That leads to a larger number of aborts.

    You can certainly get a larger pinset when you consolidate your tubs. I don't have any doubt. 

    But I've said it before, a larger pinset is not necessarily a better pinset.

    For these reasons, I choose to give it fresh air as soon as it is 100% colonized. By doing this I introduce my mycelium to its fruiting conditions as soon as possible. so that it can be "accustomed" to them and fruit as well as it can with what it has got. I hope this makes sense :thumbup:





Frank's tips and tricks :wink:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18939871 - 10/06/13 11:43 AM (10 years, 3 months ago)

I never wait for fruiting. Always fruit at 100%  I have definitely noticed a difference.

My sub shrinks significantly during colonization. Like at least  a qrt inch between tub walls and sub or more...


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18940308 - 10/06/13 01:20 PM (10 years, 3 months ago)

Quote:

Blake_Shroom said:






That one produced 8.2 oz dried first flush :laugh:



Harvested this one. Looks like its going to be more. There was some serious clusters going on




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OfflineStromriderM
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18940314 - 10/06/13 01:22 PM (10 years, 3 months ago)

Looks good Blake


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Re: 20 Tub Isolate grow [Re: Stromrider]
    #18940641 - 10/06/13 02:43 PM (10 years, 3 months ago)

Those are beautiful mushroom forests, they pin in such dense clusters! Makes me look forward to when I get to start casings, congrats man. :shroomeryhead:


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OfflineMush4Brains
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Re: 20 Tub Isolate grow [Re: OneiricOutsider]
    #18940779 - 10/06/13 03:17 PM (10 years, 3 months ago)

Damn, if only trading isolates wasn't illegal.  People would be growing so many shrooms all over the place.


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18940822 - 10/06/13 03:26 PM (10 years, 3 months ago)

Very sexy harvest pics, blake! Good job :congrats:

Quote:

Mush4Brains said:
Damn, if only trading isolates wasn't illegal.  People would be growing so many shrooms all over the place.




I wish I could sell or trade wedges from my library. It is a shame.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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OfflineMush4Brains
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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18940842 - 10/06/13 03:30 PM (10 years, 3 months ago)

I would love if you could trade or sell wedges too :lol:.

Sorry to jack your thread OP.  Good shit.


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18942591 - 10/06/13 10:14 PM (10 years, 3 months ago)



Just finished drying. That one dried to 8.75 oz


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18942862 - 10/06/13 11:26 PM (10 years, 3 months ago)

:wooyeah:

looks like things are going along just fine at shroomery. nice work. congrats. love the quality and number of pics in this thread.


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OfflineBlake_Shroom
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Re: 20 Tub Isolate grow [Re: silos]
    #18944106 - 10/07/13 10:36 AM (10 years, 3 months ago)





I'll pick the bucket today. The other tub tomorrow, and the third i'll pick in probably 3 days


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18944329 - 10/07/13 11:30 AM (10 years, 3 months ago)

Fuckin' inspired.

You're a champ.

Got my first clean LC going on right now

JARS JARS JARS JARS JARS

thumbs up for shooting big btw.


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Re: 20 Tub Isolate grow [Re: shadowman-x]
    #18948657 - 10/08/13 09:17 AM (10 years, 3 months ago)



Well, I meant to harvest this one last night, but I got really drunk watching the game so I woke up early today and harvested it. There's a lot of big ones, which is different from my usual tubs that have a ton of small fruits. While I think this can harm my average yield, these guys seemed dense as fuck. I'm pretty excited to see what they weigh when they dry



This tub seems to be sterile. There's also a lot of odd looking fruits. Hopefully tomorrow i'll have a big full canopy and i'll harvest in the morning.



Here's my bucket. 1 qrt of spawn to like 3-4 qrts cvg. Yielded just over 2 oz dried! cant believe it. It's by far the best efficiency i've had with respect to qrts of spawn to yield.


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18950702 - 10/08/13 05:36 PM (10 years, 3 months ago)

Harvest from the tub at the top of my last pic: 7.5 oz


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Re: 20 Tub Isolate grow [Re: Blake_Shroom] * 1
    #18950752 - 10/08/13 05:46 PM (10 years, 3 months ago)

Eat them all :crazy2::rocket:


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18956189 - 10/09/13 07:56 PM (10 years, 3 months ago)



9.5 oz.... my new personal record :laugh:


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18956269 - 10/09/13 08:11 PM (10 years, 3 months ago)

Sexayy


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Re: 20 Tub Isolate grow [Re: highc]
    #18958425 - 10/10/13 09:20 AM (10 years, 3 months ago)



A lot of the other tubs are pinning, but it isnt picture worthy yet. Maybe tomorrow they'll be looking real good like :thumbup:


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18958588 - 10/10/13 10:18 AM (10 years, 3 months ago)

Sir, thank you for this. It is motivating me to stop being lazy lol


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18959184 - 10/10/13 12:30 PM (10 years, 3 months ago)

Quote:

Blake_Shroom said:


9.5 oz.... my new personal record :laugh:



This whole tub was sterile... Any idea why that happened? Same culture for all these tubs. This one "stalled" for a bit when it was pinning. As you can see from my posts, my other tubs were well ahead of this one, even though they pinned 1-2 days apart.

I'm just worried that they wont be potent. I truly dont want to test them myself, i already planned an acid trip for today. i'll give some to some friends and see what they think :thumbup:

Total yield so far off of 4 tubs and 1 bucket: 34 oz dried :laugh:

all tubs that were picked were put outside since its going to rain for like the next 2 weeks. hopefully i pull an oz off of each spent tub!


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18959462 - 10/10/13 01:41 PM (10 years, 3 months ago)

Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18959781 - 10/10/13 02:43 PM (10 years, 3 months ago)

Well they are already dry. I would have loved to have sterile fruits. I think if I cloned and tried to grow out again that the culture would be way too old


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18963406 - 10/11/13 10:57 AM (10 years, 3 months ago)

Are you sure this is an isolate?

The way your tubs are varying so much between each (esp with the sterile tub) I would think your isolate my actually be a close-isolate but not quite.

Quote:

Mush4Brains said:
Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.




Not if it were an isolate, he'd have the same genetics he started with :shrug:


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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18963487 - 10/11/13 11:20 AM (10 years, 3 months ago)

Could be a spontaneous mutation. Something triggering that whole mycelial body to bypass spore production or something along those lines. Sporeless mushrooms are neat. Especially in species where spores are a health hazard, like oyster


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Re: 20 Tub Isolate grow [Re: Oeric McKenna]
    #18963531 - 10/11/13 11:31 AM (10 years, 3 months ago)

All of my other tubs + these 4 have looked the same, minus the one with the bigger ones. The sterile one is weird, I think i did something to the environment and it triggered it to not produce spores.

There is also a good chance I mislabled some petris :laugh:


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18963553 - 10/11/13 11:36 AM (10 years, 3 months ago)

Fair enough, I was just curious.

Regardless of petri labels being correct or not, you did a whole lot right here!


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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18963555 - 10/11/13 11:37 AM (10 years, 3 months ago)

Quote:

FrankHorrigan said:
Are you sure this is an isolate?

The way your tubs are varying so much between each (esp with the sterile tub) I would think your isolate my actually be a close-isolate but not quite.

Quote:

Mush4Brains said:
Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.




Not if it were an isolate, he'd have the same genetics he started with :shrug:




Yes, but if it were a genetic mutation that arose then the clone will have the mutation and you can propagate it further.


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18963565 - 10/11/13 11:39 AM (10 years, 3 months ago)

Makes sense :thumbup:

I've just never seen a spontaneous mutation in my isolates I've been working with for quite some time :shrug:


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Re: 20 Tub Isolate grow [Re: FrankHorrigan]
    #18963710 - 10/11/13 12:11 PM (10 years, 3 months ago)

I don't doubt that you haven't, something like that to affect the whole tub would have to occur early in the spawn run, I would think.  Could it be from physical conditions?  I really have no clue, I mean, I took a few genetics courses during college, but I'm no expert (especially when talking about mushroom genetics).  If it was an environmental factor, it won't be cloneable, but if it was a genetic mutation that came from a true isolate, it can be cloned I believe.


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18968767 - 10/12/13 03:18 PM (10 years, 3 months ago)


















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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18968783 - 10/12/13 03:21 PM (10 years, 3 months ago)

Do you get a lot of bottom pinning without using a liner?  Doesn't look like that bucket turned out as good as your first one :frown:.


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Re: 20 Tub Isolate grow [Re: Mush4Brains]
    #18968865 - 10/12/13 03:44 PM (10 years, 3 months ago)

Nope. I actually very rarely get bottom pins. Now side pins, that's a different story. I get a good amount of those. But it works good for me. If there are Amy bottom pins, they never mature until I toss the substrate outside and upside down.

I do have a tub with a liner going. Theres no pics of it because it hasn't even started knotting up yet. I think there's a bacteria issue with it. I may just toss it outside since its going to be rainy and humid for the next week or 2


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18981556 - 10/15/13 02:20 PM (10 years, 3 months ago)















The 2 buckets produced 1.5 oz each, not bad. I've picked this tub. Its the only one that is different shaped, and it has consistently yielded higher, although its 56 qrt instead of 66 like all my others.



I'll post yields tonight


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18981596 - 10/15/13 02:32 PM (10 years, 3 months ago)

Cool so you didn't grow these on cakes? cuz it looks lie the whole bottom is just myocil


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Edited by Kiya_Star427 (10/15/13 02:33 PM)


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18981758 - 10/15/13 03:15 PM (10 years, 3 months ago)

Quote:

Blake_Shroom said:






:crazy2:


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Re: 20 Tub Isolate grow [Re: Kiya_Star427]
    #18981779 - 10/15/13 03:20 PM (10 years, 3 months ago)

Blake you owe me a new phone. I started reading this thread and came all over the one I had.

Lol in any case very nice grow, simply gorgeous. Quite inspiring. And im so going to start using your steam pastuerizer. And great concept well done!


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Re: 20 Tub Isolate grow [Re: Kiya_Star427]
    #18981793 - 10/15/13 03:24 PM (10 years, 3 months ago)

Quote:

Kiya_Star427 said:
Cool so you didn't grow these on cakes? cuz it looks lie the whole bottom is just myocil




the subject of the thread says Tubs

Blake....do you use a protective layer of sub over your grains after spawning or nah?


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Re: 20 Tub Isolate grow [Re: DeadPhan]
    #18982077 - 10/15/13 04:27 PM (10 years, 3 months ago)

Quote:

DeadPhan said:
Quote:

Kiya_Star427 said:
Cool so you didn't grow these on cakes? cuz it looks lie the whole bottom is just myocil




the subject of the thread says Tubs

Blake....do you use a protective layer of sub over your grains after spawning or nah?



Nope. I did for a while, then tried it without, and they colonized way faster. And fast enough to where not of the grain dried out.

I'm having a real issue with water pooling at the bottom of my tubs. I'm drilling some small holes in the corner to drain. This is insane


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18985401 - 10/16/13 09:50 AM (10 years, 3 months ago)

Quote:

Blake_Shroom said:








9.5 oz dried first flush :laugh:


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #18990020 - 10/17/13 09:26 AM (10 years, 3 months ago)




Dry weights tonight


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #19008461 - 10/21/13 12:59 PM (10 years, 3 months ago)
















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Re: 20 Tub Isolate grow *DELETED* [Re: Blake_Shroom]
    #19008514 - 10/21/13 01:16 PM (10 years, 3 months ago)

Post deleted by Citric

Reason for deletion: http://www.shroomery.org/forums/showflat.php/Number/14960167 #7



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Edited by Kiya_Star427 (10/21/13 01:17 PM)


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Re: 20 Tub Isolate grow [Re: Kiya_Star427] * 1
    #19008519 - 10/21/13 01:18 PM (10 years, 3 months ago)

Imagine if there was no talk of "flipping" anything in this forum.

This is Muchroom Cultivation, not Drug Dealing 101...


Edited by Citric (10/21/13 02:44 PM)


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Re: 20 Tub Isolate grow [Re: PussyFart]
    #19008574 - 10/21/13 01:31 PM (10 years, 3 months ago)

:whathesaid:


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Re: 20 Tub Isolate grow [Re: tybojengles]
    #19009245 - 10/21/13 04:09 PM (10 years, 3 months ago)

Quote:

tybojengles said:
:whathesaid:




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Re: 20 Tub Isolate grow [Re: Brain Fart]
    #19009271 - 10/21/13 04:16 PM (10 years, 3 months ago)

Nice fruits!  :kaneclap:


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Re: 20 Tub Isolate grow [Re: ChinChiller]
    #19009418 - 10/21/13 04:56 PM (10 years, 3 months ago)

nice log bra!


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Re: 20 Tub Isolate grow [Re: PirateSwazey]
    #19009685 - 10/21/13 05:51 PM (10 years, 3 months ago)

congrats on the successful grow :mushroom2:


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Re: 20 Tub Isolate grow [Re: phxBoomer]
    #19010008 - 10/21/13 06:46 PM (10 years, 3 months ago)

So beautiful. So inspiring. Making my mouth water. Nice work Blake.


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #19010035 - 10/21/13 06:49 PM (10 years, 3 months ago)

Quote:

Blake_Shroom said:



Dry weights tonight



Those ones were 12.1 oz and 11.9 oz, respectively.

The other ones matures when I was out of town. My brother harvested them for me. I dont know the exact weights, but I do know that they averaged over 9 oz.

Second flushes outside averaged around 1 oz. Its getting cold and theres not much more rain predicted so i doubt i'll get a lot more outside


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Re: 20 Tub Isolate grow *DELETED* [Re: Kiya_Star427]
    #19010215 - 10/21/13 07:19 PM (10 years, 3 months ago)

BLAKE, looking good, homie.  Your shit is top notch :thumbup:


Edited by Citric (10/21/13 10:01 PM)


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Re: 20 Tub Isolate grow [Re: Blake_Shroom]
    #20049694 - 05/28/14 01:27 AM (9 years, 8 months ago)

Quote:

Blake_Shroom said:





There's some pins popping up. Should be quite the pinset

I'm spawning 7 tubs today and tomorrow, for a total of 16 tubs colonizing (a few already done) and 4 tubs fruiting. And 3 buckets fruiting, 2 more to be spawned this weekend too.




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Re: 20 Tub Isolate grow [Re: Brain Fart]
    #20049695 - 05/28/14 01:28 AM (9 years, 8 months ago)

Shush


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