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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 3 months
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20 Tub Isolate grow 4
#18892903 - 09/26/13 07:36 AM (10 years, 4 months ago) |
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Well, this is what i've been waiting for. In late august, I had 20 petris of an iso that I put to RGS in qrt jars. 2-3 weeks later (my house is usually 68*) I G2G'd them all to their own individual spawn bag with 5 qrts of RGS. After those colonized, I spawned them to Coir/Verm. I dont know exactly how much, but I would assume around 1 1/3 brick of coir and 4 qrts of verm all pasteurized using my bulk steam pasteurizer in sig.
4 tubs were put into fruiting 3 days ago. They're starting to knot up. Hopefully a beautiful pinset by the end of the weekend. I'm having a little issue of water pooling on the bottom of the tub. Next tubs will have a liner with a small hole drilled in a bottom corner, with tape put over it. That way when it does build up with water, i'll just take the tape off and drain. Notahacker420 had this idea




The 4 tubs were spawned on sunday. There is some really great growth going on there should be put into fruiting by sunday

These 5 tubs were made on tuesday. Just as well, some really great growth. Possibly put them into fruiting on sunday as well.

I had some extra jars that didnt look as good as my other jars that were G2G'd into spawn bags, so i made some buckets up I'll be writing a tek here soon

These 2 spawn jars will be made into buckets as well, probably Friday after I pasteurize all my substrate tomorrow

This jar is a clone from a fruit i called "gigantor", hence that is what i named the culture :P I'll G2G this into a spawn bag and fruit it into a mono to see how well it performs

It was cloned from one of the big fruits in that cluster from this tub. I can't remember exactly which one it was, but it weighed 60 grams wet on the first flush

And now to the spawn bags that are almost fully colonized. There's 7 that will be spawned on friday. There is 1 extra that got some bacteria. I stupidly put a blanket over some bags to help speed up colonization, but man oh man these bags produce some HEAT. luckily i took it off after only 1 night so only this bag is fucked. I'll probably spawn it outside, although I really havent had any luck with grain outside (i throw out my spent substrate and get great outdoor fruits). Since it's getting towards fall and i havent had any luck, i'm really not going to put any effort into spawning it outside.

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I'll update about once every 2 days, until shit starts to get real purdy.
By the way, I've grown out this ISO before and it averaged roughly 7oz dried first flush, with the second flush getting 1oz, then toss outside and pull another oz or so
Edited by Blake_Shroom (09/26/13 07:37 AM)
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Interesting. I'm looking forward to seeing the results!
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   The Corbett Report Open Source Intelligence News
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ely2121
Vagabond

Registered: 05/02/11
Posts: 385
Loc: 5th dimension
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Re: 20 Tub Isolate grow [Re: Pestile]
#18898538 - 09/27/13 12:41 PM (10 years, 4 months ago) |
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good stuff 
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Blake_Shroom
Stranger



Registered: 09/09/09
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Re: 20 Tub Isolate grow [Re: ely2121]
#18898676 - 09/27/13 01:16 PM (10 years, 4 months ago) |
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There's some pins popping up. Should be quite the pinset
I'm spawning 7 tubs today and tomorrow, for a total of 16 tubs colonizing (a few already done) and 4 tubs fruiting. And 3 buckets fruiting, 2 more to be spawned this weekend too.
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
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How many quarts do you use per bag? Is there anyway you could do a how to tek for your mycobags? I would love that! also how do you inoculate them? With agar, lc, g2g, MS etc etc
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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And here's a tub from a week ago. I had 3 tubs going, 1 contam'd right after fruiting, 1 contam'd after pins, and this one went through a full first flush and was tossed so i can cycle tubs. This was the same culture as all of the current tubs, but with 1 key difference.
I had 3 jars colonizing when i went out of town. They weren't good enough to g2g into bags at the time, so i let the colonize more. when I got back, i G2G'd them into bags, then spawned. My culture im working with now is not as "old". Rather than colonize jars, I had 20 petris made up and they sat in a fridge for well over a month, although I can tell this culture is losing its vigor.
I started this strain on agar in late march, maybe early april. I made some transfer, put to grain, and grew out a ton of plates. I took some clones, cleaned up cultures, and here we are. I chose this specific one because it was from the best cluster of the best producing tub, and I have results to prove its quality (averaging 7.5 oz dried first flush). But I took some pieces from my slants, and i can tell i need to find a new culture.
I have some maz on agar now, which i will find iso with from straight transfers, not growing out and cloning.
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 3 months
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Quote:
nn-IlliniSpiralDMT said: How many quarts do you use per bag? Is there anyway you could do a how to tek for your mycobags? I would love that! also how do you inoculate them? With agar, lc, g2g, MS etc etc
I have a tek i wrote in my signature. I only use agar when i inoculate. 5 qrts of spawn per bag, usually half a qrt of spawn when g2g, making for a total of 5 1/2 qrts of spawn per tub
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Got some pins in my tubs that are in fruiting. Looks pretty good. Gotta love all those clusters 
Also, a lot of tubs are fully colonized. I'll put those into fruiting today
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Well, 2 of them look great! 1 looks pretty decent and the other looks to be struggling. My house is pretty cold so that's why there's not much growth. I'm hoping for some dense meaty fruits 
I put 9 other tubs into fruiting today. No pins yet, some have knots. Since I dont have a good camera, I'm going to wait until pins to post pics 
Out of the other 7 tubs, 5 should be ready for fruiting by friday. The other 2 had some bacteria in the spawn bag. I dunked my grains for a while and added some extra jars into the tubs. I had the sub ready and didnt need these 2 tubs for a while, so i spawned it anyways. Worst comes to worse, the sub will colonize, then contam, then i'll throw it outside. I get way better results putting out a colonized sub outside then spawning an outdoor bed anyways
Here's one of the buckets. The other 2 have yet to knot up yet.
Edited by Blake_Shroom (10/02/13 12:59 PM)
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Stromrider
This must be the place



Registered: 06/02/13
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Loc: Dept of know what I'm say...
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Damn Blake you've been a busy beaver! Nice work
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tybojengles
Introspective Warrior



Registered: 08/02/13
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Loc: the sphincter of the worl...
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Re: 20 Tub Isolate grow [Re: Stromrider]
#18921450 - 10/02/13 01:51 PM (10 years, 4 months ago) |
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Very nice! I'm totallyz jelly.
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Well my internet if fucking up real good right now, so I can't really upload pics :/
Here's the one I could upload. At least it's the best one
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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Quote:
Blake_Shroom said:

Well my internet if fucking up real good right now, so I can't really upload pics :/
Here's the one I could upload. At least it's the best one 
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: 20 Tub Isolate grow [Re: DeadPhan]
#18930971 - 10/04/13 12:18 PM (10 years, 4 months ago) |
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Welcome to my world, Blake! 
I'm glad the isolate turned out good. I personally would not put an untested isolate to 20 tubs, but you picked a good one
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Not necessarily untested. I've done like 3 or 4 tubs with this iso. It averaged 6.5-7oz.
By the looks of these 4 tubs, i feel like my average might be lower, maybe at 5.5-6, but we'll see after I harvest this tub and it dries.
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Harvested the first one. The next one will be harvested tonight or tomorrow.
If you have seen any of my other threads, I let my guys over mature just a bit. I know this, I know its not prime picking time, but its what I like so please keep spore comments or potency comments to yourself

This is one of the 4 tubs fruited at the same time. These guys are growing so slow. Maybe a bacteria issue, i dont know. Regardless, I know they will be dense as hell when I do harvest

I think this one is going to hurt my average, but its all good. Just weird that its the same culture, but I like it being different. There's some monsters in there
Edited by Blake_Shroom (10/05/13 11:48 AM)
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Sporeo

Registered: 10/04/13
Posts: 23
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Sweet pics. Use trash bag liners next time and you won't get all those side pins. When the substrate starts to evaporate it creates a micro climate between the sub and tub wall. The humidity will be greater on the sides, leading to side pins.
Edited by Sporeo (10/05/13 12:59 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: 20 Tub Isolate grow [Re: Sporeo]
#18936050 - 10/05/13 01:14 PM (10 years, 4 months ago) |
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Quote:
Sporeo said: When the substrate starts to evaporate consolidate it creates a micro climate between the sub and tub wall.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Sporeo

Registered: 10/04/13
Posts: 23
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Re: 20 Tub Isolate grow [Re: PussyFart]
#18936086 - 10/05/13 01:26 PM (10 years, 4 months ago) |
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Haha, true. I meant to say when moisture evaluates from the substrate. Sorry lol
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: 20 Tub Isolate grow [Re: Sporeo]
#18936264 - 10/05/13 02:07 PM (10 years, 4 months ago) |
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That is not what consolidation is or why the substrate shrinks and creates that microclimate.....
You are looking for constant evaporation regardless.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (10/05/13 02:07 PM)
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