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OfflineMonkeysuckerpunch
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Registered: 08/19/13
Posts: 56
Last seen: 3 years, 5 months
Jars with strange colors, contam on moisture?
    #18890979 - 09/25/13 08:10 PM (10 years, 4 months ago)

A while ago, I used a spore syringe to inoculate some jars.  This was the second round of jars so I used some fresh syringes and some I sterilized with a flame.  My mistake was instead of inserting the syringe in and out, I got the idea that I should spread the liquid around as much as possible.  I stuck the syringe in, and spread it around back and forth as I injected the liquid.

Here is the first picture:

The yellowish color seemed unusual, but I thought since it lined up exactly with the injection site that it was just lack of BRF material itself, and maybe moisture pooled in there and turned a yellowish color.

This is the second pic:

This is a bigger example of what worried me.  Lots of BRF material was moved by the syringe, left a wider hole where the mycelium couldn't colonize it?  Or is that a contam from the syringe tip??

This last pis is the one that worries me:

The site is too big for me to have scratched the material off, it's too far down for my syringe to have reached it, and while the mycelium kept growing down around it, it never got that section filled.  It is still in line with the injection site, though.

Anyone recognize it as a contamination?  Possibly just excess moisture?  This is my first grow, and I have definitely had to throw away other jars, but those jars had really gross and easily identifiable green shit on them.


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Offlineretaardvark
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Registered: 12/22/12
Posts: 64
Last seen: 4 years, 8 months
Re: Jars with strange colors, contam on moisture? [Re: Monkeysuckerpunch]
    #18891257 - 09/25/13 09:20 PM (10 years, 4 months ago)

It seems you are using a dry verm barrier on top -- is this the case?

If so, its purpose is to prevent debris from finding its way onto your sterile cake. It seems likely that swishing the needle around disturbed the barrier, allowing little crumbs and contaminants to fall or be pushed down into your cake.

You would disturb the barrier much less, and thus have a much smaller vector for contamination, if you minimize movement of your needle through your verm barrier.

They first two pics look like they might have some bacterial action, as evidence by some pretty dense metabolite build up; though I certainly don't have the experience to be entirely confident. Last pic is inconclusive to me.


Edited by retaardvark (09/25/13 09:21 PM)


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OfflineAmanita virosa
botanist by day
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Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 17 days
Trusted Cultivator
Re: Jars with strange colors, contam on moisture? [Re: retaardvark]
    #18891527 - 09/25/13 10:11 PM (10 years, 4 months ago)

Tough call. Second jar could be bacterial in that spot or could be nuthin. Is the third jar stalled?  Looks like it. If so I would suspect bacteria.


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