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OfflineMassively Nooby
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Inexperienced Mycology
    #18888791 - 09/25/13 11:11 AM (10 years, 4 months ago)

I've only recently, past few months, become interested in mycology and mushroom cultivation as a hobby. I've had no success thus far, but today is day ten on incubation of twelve half pint jars of Huautla I ordered from the spore works. So far, I've seen no signs of growth, however when opening the plastic box I store them in I'm detecting a feint "sour" odor, an obvious contamination but as of yet I can not determine which of the jars are contamed and which, if any, aren't.

That aside, today I receive my order of a 10cc syringe of the ********** Psilocybe Cubensis Corumba Brazil. With this I wanted to try my hand at a WBS to bulk substrate. Problem being, I do not have access to a pressure cooker and I've been forced to try using fractional sterilization. Anyway, to get to the point, is fractional sterilization, if I run a course of 5-7 days, 1 hr boiled then cooled for 24 and repeated, will this give me an effective sterilization?

I understand that it's impossible to entirely sterilize all the WBS in this method, but is its effectiveness worth attempting or should I just wait to try WBS to bulk when I get a PC?

I'll update this later tonight with some pictures.


****Mod edit: no vendor names please!


Edited by stonesun (09/25/13 01:38 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Inexperienced Mycology [Re: Massively Nooby]
    #18888813 - 09/25/13 11:17 AM (10 years, 4 months ago)

Hey man remove the vendors names from your post. You don't grow from syringes they're for microscopic inspection only. Don't throw people under the bus and give the rules a good read which you should do before your first post.

Quote:

I've been forced to try using fractional sterilization. Anyway, to get to the point, is fractional sterilization, if I run a course of 5-7 days, 1 hr boiled then cooled for 24 and repeated, will this give me an effective sterilization?




This has almost never worked for anyone who had to try it for themselves just to see if they could cut the corner. As you will find out for yourself this is not a corner that's worth cutting.

Quote:

r should I just wait to try WBS to bulk when I get a PC?




you NEED the PC to do grains.


Do the PF tek in accordance with the "lets grow mushroom" vidoes/tek
http://www.shroomery.org/11432/Lets-Grow-Mushrooms-Pf-tek-videos

Follow this to the T don't skip anything or add anything. Get your first success then try other things.


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Offlinespacechildo
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18888824 - 09/25/13 11:20 AM (10 years, 4 months ago)

Don't do fractional sterilization. According to RR, it doesn't work.
What does work however, and to a satisfactory degree, is to steam
them in a regular pot for 8 hrs.

You'll probably need to add more water during the cycle,
so keep some water boiling in another kettle next to you.

Good Luck :thumbup:


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Invisibleanne halonium
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Re: Inexperienced Mycology [Re: spacechildo]
    #18888845 - 09/25/13 11:25 AM (10 years, 4 months ago)

or, ya could just do the rational thing, and get a PC.


--------------------
:aliendance:


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InvisiblebodhisattaMDiscordReddit
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Re: Inexperienced Mycology [Re: spacechildo]
    #18888850 - 09/25/13 11:25 AM (10 years, 4 months ago)

8 hours without a PC will get them to the point at which the cubes have a chance to grow, but even still they're not completely sterilized. That's just the point at which you start to see a success rate happen.


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Offlinespacechildo
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18888886 - 09/25/13 11:34 AM (10 years, 4 months ago)

Quote:

bodhisatta said:
8 hours without a PC will get them to the point at which the cubes have a chance to grow, but even still they're not completely sterilized. That's just the point at which you start to see a success rate happen.




..start to see a success rate happen? 0/10 is still a rate :wink:
RR claims to get satisfactory results this way, his webpage describes
a 55 gallon drum used to steam sterilize grains for 8 hrs.

He has a freakin farm, I think he grows more than the rest of us around here combined :grin:


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Re: Inexperienced Mycology [Re: spacechildo]
    #18888916 - 09/25/13 11:45 AM (10 years, 4 months ago)

FWIW, I thought I heard what I posted from a RR post. He said he hadn't seen contams in something like 20k blocks of substrate, but just sterilizing the jars properly is not even half the battle to success when you first start out.


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Offlinespacechildo
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18888954 - 09/25/13 11:56 AM (10 years, 4 months ago)

Quote:

bodhisatta said:
FWIW, I thought I heard what I posted from a RR post. He said he hadn't seen contams in something like 20k blocks of substrate, but just sterilizing the jars properly is not even half the battle to success when you first start out.




I don't get this.. You say RR said it does not work,
yet he has 0 contams in 20000 blocks..?

http://www.mushroomvideos.com/Wood-Fired-Steam-Boiler


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Re: Inexperienced Mycology [Re: spacechildo]
    #18888973 - 09/25/13 12:01 PM (10 years, 4 months ago)

They're only as sterilized as a PF cake at that point. It just takes 8 hours to get everything up to temp but it's still only 212 rather than 250ish. If you have really good clean spawn and a spawn at an appropriate ratio and have been doing things for a while you'll be successful but for a new grower an 8hour cycle for grain jars is the easier part of the whole thing. let alone the fact that he will probably do things like wipe with alcohol after flame sterilizing tools, shitty lids, leaving foil on the lids etc...


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Offlinespacechildo
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18889001 - 09/25/13 12:08 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
They're only as sterilized as a PF cake at that point. It just takes 8 hours to get everything up to temp but it's still only 212 rather than 250ish. If you have really good clean spawn and a spawn at an appropriate ratio and have been doing things for a while you'll be successful but for a new grower an 8hour cycle for grain jars is the easier part of the whole thing. let alone the fact that he will probably do things like wipe with alcohol after flame sterilizing tools, shitty lids, leaving foil on the lids etc...




Say what? A PF-cake is just as sterilized as grain is, it just takes
more timer or higher temp to "reach through to" the grains..

Of course, a PC would be preferable, but I'd still say to go steam for 8 hrs if pc is not an option.


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Re: Inexperienced Mycology [Re: spacechildo]
    #18889062 - 09/25/13 12:27 PM (10 years, 4 months ago)

Some endospores can survive 212

Your grain is fine if some endospores, most likely wont germinate or make if they did, but it's not fully sterilized.

http://www.shroomery.org/forums/showflat.php/Number/6605097#6605097
http://www.shroomery.org/forums/showflat.php/Number/6682322#6682322

Rice doesn't have endospores for the most part so it's cool to steam it but it's not the same as it would be if it were a PCed pf cake.



Edited by Trusted cuItivator (09/25/13 12:37 PM)


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Offlinespacechildo
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18889503 - 09/25/13 02:04 PM (10 years, 4 months ago)

I think those posts are older than his 55 gallon drum.
I don't think there's anything in those links suggesting what you're saying is right either.
Try to read the link, then answer.
Or search up newer threads where he says 8hrs steam is a-ok


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Re: Inexperienced Mycology [Re: spacechildo]
    #18890510 - 09/25/13 05:59 PM (10 years, 4 months ago)

I didn't say it wouldn't work, using the 8 hours, as it obviously has, even I said RR had 0 contams... I'm just saying it's probably not going to work for him since everything else is going to have to go smooth too. Reword my post for me and PM me it so I can edit it and make you happy.


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Offlineloki44
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Re: Inexperienced Mycology [Re: bodhisatta]
    #18890683 - 09/25/13 06:41 PM (10 years, 4 months ago)

if no pressure cooker, just do the pf-tek, better to have one bird in hand than 2 flying, chances are you are gonna fail at grains without a pressure cooker


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Re: Inexperienced Mycology [Re: bodhisatta]
    #18892816 - 09/26/13 06:43 AM (10 years, 4 months ago)

Quote:

bodhisatta said:
I didn't say it wouldn't work, using the 8 hours, as it obviously has, even I said RR had 0 contams... I'm just saying it's probably not going to work for him since everything else is going to have to go smooth too. Reword my post for me and PM me it so I can edit it and make you happy.




Turning sassy, eyh?
So you're advising against sterilizing grains because growing shrooms
has a lot of trap doors?
And it might not work because he is inexperienced?

If that's the case, why would you encourage him to spend money on a pressure cooker,
when you're already doubting his abilities to grow?
And how do you suggest he'd get his experience? By not growing?

This is just too dumb, coming from the guy who made a post about contams
being an exaggerated problem and something we shouldnt worry about!


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