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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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My first agar to grain transfer. How do these wedges look?
#18857849 - 09/18/13 12:40 PM (10 years, 5 months ago) |
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They weren't as clearly rhizomorphic as from what I transfere from but still looke fairly healthy. I since moved to not altering the lids on agar jars and just using loose lids upside down. I'm debating on going back to altered lids as it seemed these took longer and possibly are being choked out? Maybe the reason they are a bit fuzzy towards the middle?
How do you think they look otherwise?
I drank a cup of coffee before doing this. Bad idea. I don't usually drink coffee so my shaky hands made it quite a pain as if its not with jars in first place.
I made more jars but the others the wedges fell down face first.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: My first agar to grain transfer. How do these wedges look? [Re: DeadPhan]
#18857923 - 09/18/13 12:58 PM (10 years, 5 months ago) |
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looks good man. You can shake the jars right away when you put wedges in. Unless you were waiting for feedback and already knew. It's maybe fuzzy in the middle because that's where the plates started from. Did you put a grain on the plate or start with spores? if those fuzzy spots are the original growth spot of the petri you might try to cut wedges a bit further out in the future to minimize the chance of an accidental over-ran contam getting in.
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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Re: My first agar to grain transfer. How do these wedges look? [Re: bodhisatta]
#18857996 - 09/18/13 01:11 PM (10 years, 5 months ago) |
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Those are from previous plates I made transfers from. I was almost curious to watch the myc take over the agar in 3d in regards to how it just grows on top in the plate. and yes. I just wanted to get pics of the wedges as I'm always skeptical of taking a pic over the open jar even if in front of flowhood. I'm gonna shake em all up but once just to watch it colonize the whole wedge.  Thanks for the feedback!
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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FunnyLight
Nom NOm NOM



Registered: 09/12/11
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Re: My first agar to grain transfer. How do these wedges look? [Re: DeadPhan]
#18859004 - 09/18/13 04:24 PM (10 years, 5 months ago) |
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What exactly do you mean when you talk about the altered lids for the agar jars?
Did you do up Doc-T's style of poor mans agar w/ the small mason jars? Or are you talking about leaving a "loose lid" on the grain jars you inoculated with the agar wedges?
Just not 100% on what you meant and if you're opening yourself up for contams.
-------------------- The most powerful drive through the ascent of man, is pleasure in his own skill. J. Bronowski Home of delicious "Psychedelic Nyotaimori". Thanks Lemmingp for that.
 
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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Re: My first agar to grain transfer. How do these wedges look? [Re: FunnyLight]
#18859412 - 09/18/13 05:41 PM (10 years, 5 months ago) |
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Quote:
FunnyLight said: What exactly do you mean when you talk about the altered lids for the agar jars?
Did you do up Doc-T's style of poor mans agar w/ the small mason jars? Or are you talking about leaving a "loose lid" on the grain jars you inoculated with the agar wedges?
Just not 100% on what you meant and if you're opening yourself up for contams. 
Altere lids as in lids with holes and tyvek or polyfil or sfd's covering them. Yes I am using mason jars so I am referring to no pour tek. I'm talking about not altering the lids and only leavin them upside down, rubber side up and not tightened all that much to allow GE.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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Re: My first agar to grain transfer. How do these wedges look? [Re: DeadPhan]
#18888589 - 09/25/13 10:12 AM (10 years, 4 months ago) |
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How long should I see full consolidation? I'll get some pics later, although it's a lil hard as I shook up to see anything. One week later, and I expected more than this. Basically, the agar wedges have really only grew around the immediate surrounding rye berries, forming a clump or ball if my myceliated agar and rye. I honestly thought there would be much more colonized by now. I'm starting to worry about my lids. I have a 3/8 inch hole, if I'm not mistaken, in the center of lid stuffed with polyfil. This has always been just fine for me with g2gs.
Do g2gs give jars a quicker head start what with more inoculation points and all?
I'm worried some reason, as barely any of my ms syringe to grain innoculations ever even germinated, and it's been like 7 weeks. I'm wondering if maybe the hoes are too big and causing my jars to dry out? Or if I'm stuffing too tight and it's choking them out. There is still slight consolidation, but doesn't look have as hydrated as I seem to remember from past jars.
My first run of innoculations was with tyvek. Everything went great. I switched to polyfil for my g2gs and still went great. This time around my innoculations didn't do shit. Although I suspect some bad syringes as I had agar contam on certain varietys. Almost two months later and I'm still waiting on fully colonized jars to be able to g2g with. Hopefully with the agar wedges I'm just being ancy. But, shouldn't I see a lot more growth a week later?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Re: My first agar to grain transfer. How do these wedges look? [Re: DeadPhan] 1
#18888620 - 09/25/13 10:21 AM (10 years, 4 months ago) |
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Those look fine, but you would have been better off not to shake as directed above.
The reason is that when you cut off a wedge, you cut through the growing, leading edge of the mycelium. It's best to let this recover without further damage, so that the mycelium crawls off the wedge and into the grains below. It makes little difference if the wedge is upside down or right side up because it's not going to recover and grow from the middle, but from the leading edges.
Once the mycelium has crawled into a few of the grains below, shake to distribute. Shaking early with a mycelium wedge slows things down because you only get recovery later from the wedge itself, not from all the mycelium which falls off when you shake, but doesn't recover and grow. You must then wait for what's left on the agar wedge to recover and grow again before the mycelium can leap off, setting things back a few days.
The other advantage to not shaking right away is that you can see the agar wedge on top so that if green mold or another contaminant develops, you see it before you shake so you can discard that jar. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 3 months
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Re: My first agar to grain transfer. How do these wedges look? [Re: RogerRabbit]
#18888649 - 09/25/13 10:31 AM (10 years, 4 months ago) |
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Thanks RR. I actually did wait a few days to shake on most of them. A couple jars I shook right away however. And the ones I waited for I gather could have waited longer as they were only just starting to touch the immediate surrounding grains.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: My first agar to grain transfer. How do these wedges look? [Re: RogerRabbit]
#18888733 - 09/25/13 10:56 AM (10 years, 4 months ago) |
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Quote:
RogerRabbit said: Those look fine, but you would have been better off not to shake as directed above.
The reason is that when you cut off a wedge, you cut through the growing, leading edge of the mycelium. It's best to let this recover without further damage, so that the mycelium crawls off the wedge and into the grains below. It makes little difference if the wedge is upside down or right side up because it's not going to recover and grow from the middle, but from the leading edges.
Once the mycelium has crawled into a few of the grains below, shake to distribute. Shaking early with a mycelium wedge slows things down because you only get recovery later from the wedge itself, not from all the mycelium which falls off when you shake, but doesn't recover and grow. You must then wait for what's left on the agar wedge to recover and grow again before the mycelium can leap off, setting things back a few days.
The other advantage to not shaking right away is that you can see the agar wedge on top so that if green mold or another contaminant develops, you see it before you shake so you can discard that jar. RR
Good to know. I'm almost positive I got my shake wedges right away info from a TC's post.
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