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Offlinefightknightx
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PF Tek with no sterilization
    #18879821 - 09/23/13 09:42 AM (10 years, 4 months ago)

Hello fellow Shroomery members.

One night (about 4 days ago) I got bored and decided to make one PF jar using no sterilization techniques just to see what happened. I used 2:1:1 verm:brf:water and put about a 1-1/2 inch layer of dry verm on top of the substrate. I covered with ONE coffee filter and no other contaminant barriers. Inoculated in open-air only flame sterilizing the syringe.

I also have 6 PF jars that one 1 has started to germinate (only in one tiny spot) in over a week and a few days. *I used grain rice pretty much rather than flour for the 6 jars and packed the substrate down which, I believe, has stalled the jars.*

This non-sterile jar is filled with germinating myc after 5 days with no signs of contaminants.

Maybe I am just getting lucky with this one jar?





Below: The substrate with rice grain, not flour. Only germination point.


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OfflineStromriderM
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Re: PF Tek with no sterilization [Re: fightknightx]
    #18879831 - 09/23/13 09:44 AM (10 years, 4 months ago)

:lol: Oh lord :facepalm:


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: fightknightx]
    #18879833 - 09/23/13 09:44 AM (10 years, 4 months ago)

Its only been 4 days dont count yourself lucky yet.
You need to grind the rice up to flour for cakes to work


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: Stromrider]
    #18879835 - 09/23/13 09:45 AM (10 years, 4 months ago)

Quote:

psillyshroomer said:
:lol: Oh lord :facepalm:



I know eh?
All day everyday:shrug:


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OfflineStromriderM
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18879841 - 09/23/13 09:47 AM (10 years, 4 months ago)

I am curious to see what happens to them though. What made you want to do this if you don't mind me asking because if sterilization wasn't necessary then trust me we would not go to the trouble


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: Stromrider]
    #18879846 - 09/23/13 09:49 AM (10 years, 4 months ago)

Its gonna contam.
I say the 6-7 day mark.


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OfflineJamesSpawned
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Re: PF Tek with no sterilization [Re: Stromrider]
    #18879851 - 09/23/13 09:50 AM (10 years, 4 months ago)

lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: JamesSpawned]
    #18879856 - 09/23/13 09:51 AM (10 years, 4 months ago)

Quote:

JamesSpawned said:
lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...



QFT
This...


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InvisiblebodhisattaMDiscordReddit
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18879895 - 09/23/13 10:05 AM (10 years, 4 months ago)

Nah man this will work great. Just open it up after 100% and take a good big sniff.


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Offlinefightknightx
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18880206 - 09/23/13 11:32 AM (10 years, 4 months ago)

Quote:

PrinceShroom said:
Quote:

JamesSpawned said:
lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...



QFT
This...



It was def. just for shits and giggs. Really the point of this post was because I was curious to see if it could happen, maybe that 1% chance. I had an extra syringe that I'm not interested in growing and really just went with it.

I will update in a few days and see if it contaminates or not.


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Offlinefightknightx
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18880229 - 09/23/13 11:36 AM (10 years, 4 months ago)

Quote:

PrinceShroom said:
Its gonna contam.
I say the 6-7 day mark.



See post above for reason.


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: fightknightx]
    #18880357 - 09/23/13 12:19 PM (10 years, 4 months ago)

Yea lets see.

I think your 1% chance is super high too.  Probably more like .0000001%


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InvisiblePestile

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Re: PF Tek with no sterilization [Re: fightknightx]
    #18880397 - 09/23/13 12:30 PM (10 years, 4 months ago)

I don't quite get it? Why would anyone do such a thing?


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: Pestile]
    #18880409 - 09/23/13 12:33 PM (10 years, 4 months ago)

For an epic fail time and LULZ?:shrug:


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OfflineJamesSpawned
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Re: PF Tek with no sterilization [Re: Pestile] * 1
    #18880411 - 09/23/13 12:34 PM (10 years, 4 months ago)

Quote:

Pestilence said:
I don't quite get it? Why would anyone do such a thing?




People gamble and play the lottery everyday, sure the odds are better in vegas but the stakes are pretty low here...


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Re: PF Tek with no sterilization [Re: JamesSpawned]
    #18880655 - 09/23/13 01:36 PM (10 years, 4 months ago)

I was actually curious about this as well, so when I made my jars I didn't sterilize or inoculate one of them. Within a couple days it looked like I was growing a fruitcake!


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InvisibleSkinty
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Re: PF Tek with no sterilization [Re: tybojengles] * 2
    #18880672 - 09/23/13 01:39 PM (10 years, 4 months ago)

4 days ago? I dunno what this discussion is about when it contams...that thing isn't covered with cubensis mycelium...:peyotezen:


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InvisiblePestile

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Re: PF Tek with no sterilization [Re: fightknightx]
    #18880819 - 09/23/13 02:11 PM (10 years, 4 months ago)

I think someone needs to re-read about the basic principles of growing mushrooms. This is a waste of time for everyone, including you.


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Offlineheratogwea
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Re: PF Tek with no sterilization [Re: Pestile]
    #18881515 - 09/23/13 05:41 PM (10 years, 4 months ago)

Why all the pretentious replys? Let the guy have his fun.


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InvisibleThe Psyentist
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18881584 - 09/23/13 06:00 PM (10 years, 4 months ago)

Quote:

PrinceShroom said:

You need to grind the rice up to flour for cakes to work




No you don't.


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OfflineMush4Brains
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Re: PF Tek with no sterilization [Re: The Psyentist]
    #18881609 - 09/23/13 06:08 PM (10 years, 4 months ago)



Yeah, that looks like real healthy myc. :lolsy:

I bet that coffee filter is doing it's job keeping contams out too.


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InvisiblePestile

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Re: PF Tek with no sterilization [Re: heratogwea] * 1
    #18883639 - 09/24/13 06:40 AM (10 years, 4 months ago)

Quote:

heratogwea said:
Why all the pretentious replys? Let the guy have his fun.




That's what happens when you post stupid shit in Mushroom Cultivation.


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Offlineheratogwea
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Re: PF Tek with no sterilization [Re: Pestile]
    #18885796 - 09/24/13 04:56 PM (10 years, 4 months ago)

Stupid is a relative term.


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OfflineMush4Brains
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Re: PF Tek with no sterilization [Re: heratogwea]
    #18885817 - 09/24/13 05:02 PM (10 years, 4 months ago)

Yeah, and this is relatively stupid as shit.  You can see what looks like mold growing on the side of the jar.  He's using a coffee filter as a filter, and he didn't even bother sterilizing his jars.  That isn't even stupid, it is ignorant and a slap in the face to everybody who has tried to improve cultivation before him. 

It's like when a professor says "there is no such thing as a stupid question."  Yes there is.  This is fucking stupid.  Stop coddling the idiots who are wasting space on Mush Cult.


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InvisiblePestile

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Re: PF Tek with no sterilization [Re: heratogwea]
    #18885833 - 09/24/13 05:06 PM (10 years, 4 months ago)

Quote:

heratogwea said:
Stupid is a relative term.




Maybe, but shit isn't. Everyone knows what shit is.


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Offlineheratogwea
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Re: PF Tek with no sterilization [Re: Mush4Brains]
    #18885841 - 09/24/13 05:06 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Yeah, and this is relatively stupid as shit.  You can see what looks like mold growing on the side of the jar.  He's using a coffee filter as a filter, and he didn't even bother sterilizing his jars.  That isn't even stupid, it is ignorant and a slap in the face to everybody who has tried to improve cultivation before him. 

It's like when a professor says "there is no such thing as a stupid question."  Yes there is.  This is fucking stupid.  Stop coddling the idiots who are wasting space on Mush Cult.




Read the op. He noced up a jar not "jars".

Cant believe all the hostility. .. on a forum dedicated to magic mushrooms no less :shrug:

You would think the guy deficated on your mothers grave judging by some of your reactions.

Whatever strokes your E ego I guess..


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OfflineMush4Brains
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Re: PF Tek with no sterilization [Re: Pestile]
    #18885845 - 09/24/13 05:07 PM (10 years, 4 months ago)

Quote:

Pestilence said:
Quote:

heratogwea said:
Stupid is a relative term.




Maybe, but shit isn't. Everyone knows what shit is.




:lol:

"This non-sterile jar is filled with germinating myc after 5 days with no signs of contaminants."


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OfflineGiggle_Grower
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Re: PF Tek with no sterilization [Re: Mush4Brains]
    #18886131 - 09/24/13 06:22 PM (10 years, 4 months ago)

Why! WHY!
Honestly, I did something similar to this and many many other stupid horrible things growin mushies when I started. Of course that was before I found the Shroomery. Thats how I learned they were stupid and horrible things. I can't  imagine doing those again, intentionally.


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OfflineJamesSpawned
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Re: PF Tek with no sterilization [Re: Giggle_Grower]
    #18887002 - 09/24/13 10:15 PM (10 years, 4 months ago)

This could be considered an endeavor to discover what types of nasties are most prolific in OPs materials/ grow space.


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InvisiblePestile

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Re: PF Tek with no sterilization [Re: JamesSpawned]
    #18887426 - 09/24/13 11:58 PM (10 years, 4 months ago)

Yeah, because there's not 470.000 dirty spores in the jar so finding out which is which will be a small breeze.

Anyway I'm done with this thread.:thumbdown:


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OfflineBLINKfan420
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Re: PF Tek with no sterilization [Re: Pestile]
    #18887736 - 09/25/13 03:07 AM (10 years, 4 months ago)

So if it colonizes fully with no contams?... lols jk. but I figure let him be. fun is fun.


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OfflineJamesSpawned
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Re: PF Tek with no sterilization [Re: Pestile]
    #18887782 - 09/25/13 03:31 AM (10 years, 4 months ago)

Quote:

Pestilence said:
Yeah, because there's not 470.000 dirty spores in the jar so finding out which is which will be a small breeze.

Anyway I'm done with this thread.:thumbdown:




Lol, I said "prolific". As in which has best the ability to proliferate in that environment etc. etc. The most prolific nasty present will very easily be identifiable because by the very definition it will have to overcome a large percent of the jar to beat out its competitors at that point becoming visually recognizable and also the most prolific blah blah. Jeeze it's just an observational experiment, certainly not deserving of all the heat it's receiving...


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Re: PF Tek with no sterilization [Re: The Psyentist]
    #18887816 - 09/25/13 03:49 AM (10 years, 4 months ago)

Quote:

The Psyentist said:
Quote:

PrinceShroom said:

You need to grind the rice up to flour for cakes to work




No you don't.





Yeah you really do. The rice won't hydrate with the minimal amount of water used in the BRF tech, and the oils wont release either. Kinda dumb to leave the rice whole.

  OP I think you should make some Agar trays with out any sterile techniques, then around day 5 you might be amazed at what is floating around the air.


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OfflinePrinceShroom
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Re: PF Tek with no sterilization [Re: Sockadin]
    #18888000 - 09/25/13 06:31 AM (10 years, 4 months ago)

are
Quote:

JamesSpawned said:
This could be considered an endeavor to discover what types of nasties are most prolific in OPs materials/ grow space.




This isnt going to show what nasties are present in the OP's grow space.  Just what contams are present in the BRF.
Quote:

Sockadin said:

  OP I think you should make some Agar trays with out any sterile techniques, then around day 5 you might be amazed at what is floating around the air.



This is more what you do to find out what is present in your area.  Take a plate and open it for a couple mins and close it back up and incubate.
Its interesting what you can see.


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OfflineJamesSpawned
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Re: PF Tek with no sterilization [Re: PrinceShroom]
    #18888732 - 09/25/13 10:56 AM (10 years, 4 months ago)

Quote:

PrinceShroom said:
are
Quote:

JamesSpawned said:
This could be considered an endeavor to discover what types of nasties are most prolific in OPs MATERIALS/ grow space.




This isnt going to show what nasties are present in the OP's grow space.  Just what contams are present in the BRF.




My bad, I assumed materials would cover the BRF. Which there actually is none OP left the rice whole, but I catch your drift.


Edited by JamesSpawned (09/25/13 11:20 AM)


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InvisibleThe Psyentist
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Re: PF Tek with no sterilization [Re: Sockadin]
    #18890253 - 09/25/13 04:56 PM (10 years, 4 months ago)

Quote:

Sockadin said:
Quote:

The Psyentist said:
Quote:

PrinceShroom said:

You need to grind the rice up to flour for cakes to work




No you don't.





Yeah you really do. The rice won't hydrate with the minimal amount of water used in the BRF tech, and the oils wont release either. Kinda dumb to leave the rice whole.






No. You really don't. I had a situation in the past where my grinder broke and I needed to start my jars. So I used whole rice and had no issues. Don't assume things.


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Offlinemastercultivator
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Re: PF Tek with no sterilization [Re: The Psyentist]
    #18890763 - 09/25/13 07:00 PM (10 years, 4 months ago)

Quote:

The Psyentist said:
Quote:

Sockadin said:
Quote:

The Psyentist said:
Quote:

PrinceShroom said:

You need to grind the rice up to flour for cakes to work




No you don't.





Yeah you really do. The rice won't hydrate with the minimal amount of water used in the BRF tech, and the oils wont release either. Kinda dumb to leave the rice whole.






No. You really don't. I had a situation in the past where my grinder broke and I needed to start my jars. So I used whole rice and had no issues. Don't assume things.




Simmer the grain if you can first.

I'm guessing the pf jars above were cooked somehow.  Might work out unless a contam germs in the meantime and takes over.  The steaming / cooking is just fine.  You can steam your jars if you use fine flour. 

I think some readers think that you cooked your mix separately and loaded it in an unsterile environ or didn't cook the pf jars at all.  Then it wouldn't work.  At any rate so far it has taken off but who knows?  time will tell.


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Offlineloki44
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Re: PF Tek with no sterilization [Re: mastercultivator]
    #18890779 - 09/25/13 07:07 PM (10 years, 4 months ago)

battle of contams! in to see what results OP end up with...


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Offlinefungi-funguy
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Re: PF Tek with no sterilization [Re: fightknightx]
    #22899609 - 02/13/16 12:26 PM (7 years, 11 months ago)

I know this thread is two years old but did it not end up working for you? There's a guy on YouTube that inoculates a bag with rye then transfers that into another bag with moistened hardwood pellets without sterilizing the pellets. He has had great results. Has anyone else done this without sterilizing it?


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OfflineGreenRabbit
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Re: PF Tek with no sterilization [Re: fungi-funguy]
    #22899886 - 02/13/16 01:57 PM (7 years, 11 months ago)

This is a jar that wasn't sterilized or inoculated.
This is what you are trying to fight...



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