|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
fightknightx
Stranger

Registered: 09/10/13
Posts: 15
Last seen: 17 days, 4 hours
|
PF Tek with no sterilization
#18879821 - 09/23/13 09:42 AM (10 years, 5 months ago) |
|
|
Hello fellow Shroomery members.
One night (about 4 days ago) I got bored and decided to make one PF jar using no sterilization techniques just to see what happened. I used 2:1:1 verm:brf:water and put about a 1-1/2 inch layer of dry verm on top of the substrate. I covered with ONE coffee filter and no other contaminant barriers. Inoculated in open-air only flame sterilizing the syringe.
I also have 6 PF jars that one 1 has started to germinate (only in one tiny spot) in over a week and a few days. *I used grain rice pretty much rather than flour for the 6 jars and packed the substrate down which, I believe, has stalled the jars.*
This non-sterile jar is filled with germinating myc after 5 days with no signs of contaminants.
Maybe I am just getting lucky with this one jar?


Below: The substrate with rice grain, not flour. Only germination point.
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 20 hours, 54 minutes
|
Re: PF Tek with no sterilization [Re: fightknightx]
#18879831 - 09/23/13 09:44 AM (10 years, 5 months ago) |
|
|
Oh lord
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: fightknightx]
#18879833 - 09/23/13 09:44 AM (10 years, 5 months ago) |
|
|
Its only been 4 days dont count yourself lucky yet. You need to grind the rice up to flour for cakes to work
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: Stromrider]
#18879835 - 09/23/13 09:45 AM (10 years, 5 months ago) |
|
|
Quote:
psillyshroomer said:
Oh lord 
I know eh? All day everyday
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 20 hours, 54 minutes
|
Re: PF Tek with no sterilization [Re: PrinceShroom]
#18879841 - 09/23/13 09:47 AM (10 years, 5 months ago) |
|
|
I am curious to see what happens to them though. What made you want to do this if you don't mind me asking because if sterilization wasn't necessary then trust me we would not go to the trouble
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: Stromrider]
#18879846 - 09/23/13 09:49 AM (10 years, 5 months ago) |
|
|
Its gonna contam. I say the 6-7 day mark.
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
JamesSpawned
Fart smeller! Wait...



Registered: 01/15/13
Posts: 773
Loc: Treasure Coast
Last seen: 7 years, 2 months
|
Re: PF Tek with no sterilization [Re: Stromrider]
#18879851 - 09/23/13 09:50 AM (10 years, 5 months ago) |
|
|
lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...
-------------------- ARE YOU NOT ENTERTAINED?!
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: JamesSpawned]
#18879856 - 09/23/13 09:51 AM (10 years, 5 months ago) |
|
|
Quote:
JamesSpawned said: lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...
QFT This...
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: PF Tek with no sterilization [Re: PrinceShroom]
#18879895 - 09/23/13 10:05 AM (10 years, 5 months ago) |
|
|
Nah man this will work great. Just open it up after 100% and take a good big sniff.
|
fightknightx
Stranger

Registered: 09/10/13
Posts: 15
Last seen: 17 days, 4 hours
|
Re: PF Tek with no sterilization [Re: PrinceShroom]
#18880206 - 09/23/13 11:32 AM (10 years, 5 months ago) |
|
|
Quote:
PrinceShroom said:
Quote:
JamesSpawned said: lol I'm not bashing it looks like you did this just for shits and giggles anyway but in my experience most mold spores take roughly 8-10 days to manifest into a nasty. Just my spare change though, good luck man...
QFT This...
It was def. just for shits and giggs. Really the point of this post was because I was curious to see if it could happen, maybe that 1% chance. I had an extra syringe that I'm not interested in growing and really just went with it.
I will update in a few days and see if it contaminates or not.
|
fightknightx
Stranger

Registered: 09/10/13
Posts: 15
Last seen: 17 days, 4 hours
|
Re: PF Tek with no sterilization [Re: PrinceShroom]
#18880229 - 09/23/13 11:36 AM (10 years, 5 months ago) |
|
|
Quote:
PrinceShroom said: Its gonna contam. I say the 6-7 day mark.
See post above for reason.
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: fightknightx]
#18880357 - 09/23/13 12:19 PM (10 years, 5 months ago) |
|
|
Yea lets see.
I think your 1% chance is super high too. Probably more like .0000001%
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
|
Re: PF Tek with no sterilization [Re: fightknightx]
#18880397 - 09/23/13 12:30 PM (10 years, 5 months ago) |
|
|
I don't quite get it? Why would anyone do such a thing?
--------------------
   The Corbett Report Open Source Intelligence News
|
PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: PF Tek with no sterilization [Re: Pestile]
#18880409 - 09/23/13 12:33 PM (10 years, 5 months ago) |
|
|
For an epic fail time and LULZ?
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
JamesSpawned
Fart smeller! Wait...



Registered: 01/15/13
Posts: 773
Loc: Treasure Coast
Last seen: 7 years, 2 months
|
Re: PF Tek with no sterilization [Re: Pestile] 1
#18880411 - 09/23/13 12:34 PM (10 years, 5 months ago) |
|
|
Quote:
Pestilence said: I don't quite get it? Why would anyone do such a thing?
People gamble and play the lottery everyday, sure the odds are better in vegas but the stakes are pretty low here...
-------------------- ARE YOU NOT ENTERTAINED?!
|
tybojengles
Introspective Warrior



Registered: 08/02/13
Posts: 30
Loc: the sphincter of the worl...
Last seen: 9 years, 8 months
|
Re: PF Tek with no sterilization [Re: JamesSpawned]
#18880655 - 09/23/13 01:36 PM (10 years, 5 months ago) |
|
|
I was actually curious about this as well, so when I made my jars I didn't sterilize or inoculate one of them. Within a couple days it looked like I was growing a fruitcake!
|
Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
|
Re: PF Tek with no sterilization [Re: tybojengles] 2
#18880672 - 09/23/13 01:39 PM (10 years, 5 months ago) |
|
|
4 days ago? I dunno what this discussion is about when it contams...that thing isn't covered with cubensis mycelium...
|
Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
|
Re: PF Tek with no sterilization [Re: fightknightx]
#18880819 - 09/23/13 02:11 PM (10 years, 5 months ago) |
|
|
I think someone needs to re-read about the basic principles of growing mushrooms. This is a waste of time for everyone, including you.
--------------------
   The Corbett Report Open Source Intelligence News
|
heratogwea



Registered: 05/05/11
Posts: 607
Last seen: 3 years, 9 months
|
Re: PF Tek with no sterilization [Re: Pestile]
#18881515 - 09/23/13 05:41 PM (10 years, 4 months ago) |
|
|
Why all the pretentious replys? Let the guy have his fun.
|
The Psyentist
Figment of my Imagination


Registered: 07/30/13
Posts: 82
Loc: Central Florida
|
Re: PF Tek with no sterilization [Re: PrinceShroom]
#18881584 - 09/23/13 06:00 PM (10 years, 4 months ago) |
|
|
Quote:
PrinceShroom said:
You need to grind the rice up to flour for cakes to work
No you don't.
--------------------
|
|