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Offlineshqhgh123
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Registered: 09/20/13
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What to do for leftover syringe solution?
    #18877549 - 09/22/13 07:32 PM (10 years, 5 months ago)

Hello,
I think I'll have more than enough solution for inoculation. (probably about 2~4cc)
So after inoculating, can I flame/alcohol sterilize the needle, put the cap back on and throw that in a fridge? Would this cause contamination later?


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OfflineKitten on Crack
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Registered: 06/23/13
Posts: 38
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Last seen: 1 year, 4 months
Re: What to do for leftover syringe solution? [Re: shqhgh123]
    #18877563 - 09/22/13 07:34 PM (10 years, 5 months ago)

You pretty much got it,
Flame sterilize the needle, put the cap back on and store in a cool place (room temp will do fine)


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Offlinemyplague240


Registered: 03/17/13
Posts: 127
Last seen: 10 years, 3 months
Re: What to do for leftover syringe solution? [Re: shqhgh123]
    #18877568 - 09/22/13 07:35 PM (10 years, 5 months ago)

If I were you I'd put a brand new needle on after use, then just store it in a zip lock baggie in the fridge. :thumbup:


Don't use alcohol.. It sanitizes not sterilizes.


Edited by myplague240 (09/22/13 07:36 PM)


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OfflineMush4Brains
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Registered: 07/31/13
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Re: What to do for leftover syringe solution? [Re: shqhgh123]
    #18877569 - 09/22/13 07:35 PM (10 years, 5 months ago)

As long as you don't bring in contaminated air you should be fine.


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Offlineshqhgh123
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Registered: 09/20/13
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Re: What to do for leftover syringe solution? [Re: Mush4Brains]
    #18877615 - 09/22/13 07:45 PM (10 years, 5 months ago)

OK thanks guys.
One more question, does injecting more than 2cc of spore solution into each PFtek jar accelerate the colonization?
If it does than I'd rather speed up the process rather than risking contamination from reusing it.


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Offlinemyplague240


Registered: 03/17/13
Posts: 127
Last seen: 10 years, 3 months
Re: What to do for leftover syringe solution? [Re: shqhgh123]
    #18877754 - 09/22/13 08:13 PM (10 years, 5 months ago)

1cc or so is a good amount to use. It doesn't really matter when using MS syringes, as it only takes 2 spores for mycelium to start forming. The most important thing to consider when making BRF jars is proper sterilization!  :thumbup:


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OfflineMush4Brains
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Re: What to do for leftover syringe solution? [Re: myplague240]
    #18877765 - 09/22/13 08:16 PM (10 years, 5 months ago)

More spores does not increase colonization times nor yield.


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