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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit?
    #18876454 - 09/22/13 03:37 PM (10 years, 5 months ago)

I've used the search function to try and find an answer. Most of the people who asked similar questions were looking to store a mushroom for days or longer before cloning. I just want to know if it's alright to wait 12 hours after harvesting before I clone. Perhaps I could refrigerate the mushroom overnight? I don't have the option to wait longer for harvesting because I can only access fruiting tub at night and only access SAB in morning(bringing SAB where tub is fruiting is also not an option)


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OfflineSupaThaRipper
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: bulkgrownoob]
    #18876516 - 09/22/13 03:49 PM (10 years, 5 months ago)

Im sure it would be fine


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Offlinedark3st
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Registered: 08/02/13
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: SupaThaRipper]
    #18876576 - 09/22/13 04:01 PM (10 years, 5 months ago)

You can always rehydrate 'em if anything


--------------------
Back.. I'm going to do it...I'm getting sober from opiates ... I got weed, gabapentin, propranolol, and GHB, I have 100mg tramadol left. I can do this. I can do this.

OFINTQWGVGAKGCYKBUBX
free dark P. Tampanensis prints to ODD members.

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I have these seeds:
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OfflineChuckfinely
another round for me an my buddy

Registered: 06/27/13
Posts: 628
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: dark3st]
    #18876598 - 09/22/13 04:07 PM (10 years, 5 months ago)

worse case and you would just have to do a couple extra transfers in order to clean the culture up. just want to catch it in time so that the myc is still alive


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: dark3st]
    #18876620 - 09/22/13 04:13 PM (10 years, 5 months ago)

Quote:

dark3st said:
You can always rehydrate 'em if anything




Your mushrooms won't dehydrate in twelve hours by themselves.


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Offlinedark3st
Stranger

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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: Mush4Brains]
    #18876636 - 09/22/13 04:16 PM (10 years, 5 months ago)

They lose +/- 20% of water within that time if on newspaper.


--------------------
Back.. I'm going to do it...I'm getting sober from opiates ... I got weed, gabapentin, propranolol, and GHB, I have 100mg tramadol left. I can do this. I can do this.

OFINTQWGVGAKGCYKBUBX
free dark P. Tampanensis prints to ODD members.

no stamps atm

FREE SEEDS for ODD WCA  members ONLY

I have these seeds:
Orange, red, and yellow sweet peppers, Purple poppies, White Habanero, Yellow Thai, Bolivian rainbow peppers, milk thistle, red chilly pepper, HBWR.


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OfflineSupaThaRipper
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: dark3st]
    #18876710 - 09/22/13 04:32 PM (10 years, 5 months ago)

Then dont put em on newspaper....


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: SupaThaRipper]
    #18876713 - 09/22/13 04:33 PM (10 years, 5 months ago)

Quote:

bulkgrownoob said:
I've used the search function to try and find an answer. Most of the people who asked similar questions were looking to store a mushroom for days or longer before cloning. I just want to know if it's alright to wait 12 hours after harvesting before I clone. Perhaps I could refrigerate the mushroom overnight? I don't have the option to wait longer for harvesting because I can only access fruiting tub at night and only access SAB in morning(bringing SAB where tub is fruiting is also not an option)





Put it in a ziplock bag and stick it in the fridge. It'll be good for days.

I've done it a million times like this.

Ok, not literally "a million" times...but you know what I mean :rofl:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (09/22/13 04:40 PM)


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Offlinedark3st
Stranger

Registered: 08/02/13
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Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: SupaThaRipper]
    #18876742 - 09/22/13 04:38 PM (10 years, 5 months ago)

Quote:

SupaThaRipper said:
Then dont put em on newspaper....




:facepalm: I'm saying depending on where you place 'em it could really change how much water stays or goes.

But ya refrigerator dude.


--------------------
Back.. I'm going to do it...I'm getting sober from opiates ... I got weed, gabapentin, propranolol, and GHB, I have 100mg tramadol left. I can do this. I can do this.

OFINTQWGVGAKGCYKBUBX
free dark P. Tampanensis prints to ODD members.

no stamps atm

FREE SEEDS for ODD WCA  members ONLY

I have these seeds:
Orange, red, and yellow sweet peppers, Purple poppies, White Habanero, Yellow Thai, Bolivian rainbow peppers, milk thistle, red chilly pepper, HBWR.


Extras: Filter Print Post Top
OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,681
Loc: USA Flag
Last seen: 3 hours, 12 minutes
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: dark3st]
    #18876768 - 09/22/13 04:43 PM (10 years, 5 months ago)

:wink:


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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: SupaThaRipper]
    #18876841 - 09/22/13 05:01 PM (10 years, 5 months ago)

Thanks for the responses everyone. I'm gonna go with the ziplock in the fridge. Wish me luck in my first agar experiment  :elmo:


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: bulkgrownoob]
    #18876897 - 09/22/13 05:13 PM (10 years, 5 months ago)

:goodluck:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: FrankHorrigan]
    #18876916 - 09/22/13 05:16 PM (10 years, 5 months ago)

Quote:

FrankHorrigan said:
:goodluck:



Thanks frank. I read your cloning tek a few times but I have one question about it.. Do you clean your gloves somehow in between touching the mushroom and touching your scalpel/Petri dish? I'm a little worried about transferring contams into my dish after holding the mushroom with both hands


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: bulkgrownoob]
    #18876931 - 09/22/13 05:19 PM (10 years, 5 months ago)

No, I do it exactly as I describe.

Don't directly touch the inside of the stipe where you are taking tissue from. Handle it carefully.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: bulkgrownoob]
    #18876933 - 09/22/13 05:19 PM (10 years, 5 months ago)

You shouldn't be touching the sample you're putting on a plate with anything but clean tools.  Touching one end of a scalpel or the top of a petri dish to open it will not spread contaminants.


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Invisiblebulkgrownoob
Registered: 03/21/11
Posts: 345
Re: Cloning tissue to agar: Okay to wait 12 hours after harvesting fruit? [Re: Mush4Brains]
    #18876960 - 09/22/13 05:24 PM (10 years, 5 months ago)

Ok thanks guys


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