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Invisibleliamtheloser
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Re: Liquid media questions [Re: drake89]
    #18873640 - 09/21/13 08:09 PM (10 years, 5 months ago)

Quote:

drake89 said:
everyone asking questions in this thread should buy a copy of Growing gourmet and medicinal mushrooms by paul stamets.  this topic is one of the early ones covered in the book.  he's all about eberbach (sp?) blenders, which are just $750 stainless steel blenders.  You can substitute this for an oster blender with the blade attached to a small mouth mason jar.  Use the regular metal jar ring instead of the plastic oster base, as it will melt in the PC.




I have it, but I didn't see the part where he uses the stainless steel blenders 24/7 on a culture.

I'm rotating my karo cultures on my stir plate now.  I only have one stir plate :/

Thanks OICU


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OfflineOICU812
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Re: Liquid media questions [Re: drake89]
    #18873745 - 09/21/13 08:34 PM (10 years, 5 months ago)

Quote:

drake89 said:
How long do you incubate?  i tried 48 hours and it didn't seem to accelerate anything.  How do the 'pros' that sell syringes get their so clear?  It's usually malt extract solution with a little 'worm' of more solid myc in it.  Maybe they don't use all of the additives recomended in ggmm, or perhaps there's a sterile filter.




1.5g LME/liter of H2O.  2 days is not enough.  Go longer and observe daily.


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"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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Invisibleliamtheloser
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Re: Liquid media questions [Re: OICU812]
    #18876180 - 09/22/13 02:30 PM (10 years, 5 months ago)

Quote:

OICU812 said:
Quote:

liamtheloser said:
So the stirring doesn't hurt the mycelium? I figured it would need time between stirs to recover,  but if not I'll keep mine on the stir plate all day.




Doesn't hurt anything.  Stir continuously. :thumbup:




So I put my starter culture of King oyster on the stir plate yesterday afternoon,  and I just went to check on it,  and there's no more fluffy mycelium, it's all condensed and dark in color.  Does the liquid culture have to be mature to be on the stir plate continuously? Because this all looks fried.


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OfflineOICU812
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Re: Liquid media questions [Re: liamtheloser]
    #18876621 - 09/22/13 04:13 PM (10 years, 5 months ago)

Quote:

liamtheloser said:
Quote:

OICU812 said:
Quote:

liamtheloser said:
So the stirring doesn't hurt the mycelium? I figured it would need time between stirs to recover,  but if not I'll keep mine on the stir plate all day.




Doesn't hurt anything.  Stir continuously. :thumbup:




So I put my starter culture of King oyster on the stir plate yesterday afternoon,  and I just went to check on it,  and there's no more fluffy mycelium, it's all condensed and dark in color.  Does the liquid culture have to be mature to be on the stir plate continuously? Because this all looks fried.





Mature?  If you'll notice in GGMM Pg. 138 Stamets goes from chopping in the Eberbach directly into the Erlenmeyer and on a stir plate.  Sounds like a possible contamination.  Dark?  As in milky-brown, dark brown, black?  Any pictures?

Put a few drops on  petri and see what develops.


--------------------
--------------
"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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Invisibleliamtheloser
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Posts: 1,453
Re: Liquid media questions [Re: OICU812]
    #18876815 - 09/22/13 04:54 PM (10 years, 5 months ago)

Well it was about a half inch thick with fluffy white mycelium, now its 2mm of brownish condensed mycelium.  Hard to tell exact color since my LC has coffee in it :wink:

It's not likely contaminated, so I'll just wait to see what transpires in the next few days with the stirplate off.


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OfflineShroomburger
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Registered: 07/10/11
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Re: Liquid media questions [Re: Shroomburger]
    #18877962 - 09/22/13 09:10 PM (10 years, 5 months ago)

Is there a minimum mass of mycelium that should be transfered to the liquid media or can I conceivably start with one cell?


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