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benny9922
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Registered: 06/22/13
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*DELETED* *DELETED*
#18873722 - 09/21/13 08:28 PM (10 years, 5 months ago) |
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Post deleted by benny9922<p>Reason for deletion: I
-------------------- All you need in life is "health" and with that you can have anything!
Edited by benny9922 (06/10/14 11:07 PM)
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SupaThaRipper
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Registered: 09/02/13
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Re: Growing mycelium on agar [Re: benny9922]
#18874819 - 09/22/13 06:32 AM (10 years, 5 months ago) |
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Contamination
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OICU812
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Registered: 11/06/11
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The first photo seems to show three wedges or samples of mycelial growth on an agar plate with the dark spot in the middle being a contaminate. Also, in the bottom left hand corner there appears to be several spots of contact resulting in growing contaminants.
The other four photos seem to show spontaneous contaminate growth from airborne origins. No physical contact with the agar media is observed.
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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benny9922
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Post deleted by benny9922<p>Reason for deletion: I
-------------------- All you need in life is "health" and with that you can have anything!
Edited by benny9922 (06/10/14 11:08 PM)
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OICU812
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Re: Growing mycelium on agar [Re: benny9922]
#18876579 - 09/22/13 04:02 PM (10 years, 5 months ago) |
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Quote:
benny9922 said: Ok thanks mate ! The first picture was my original growth and the wedges missing are what was grown on the agar plates below
Keep working it. You can usually clone away from contamination in two or three tries providing your procedure is clean.
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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benny9922
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Re: Growing mycelium on agar *DELETED* [Re: OICU812]
#18878188 - 09/22/13 09:57 PM (10 years, 5 months ago) |
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Post deleted by benny9922Reason for deletion: I
-------------------- All you need in life is "health" and with that you can have anything!
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matsc
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Re: Growing mycelium on agar [Re: benny9922]
#18878900 - 09/23/13 01:14 AM (10 years, 4 months ago) |
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Mycelium (both the kind you want and that of contaminant molds) will look like fine fluffy cottony strands. They may grow on the surface of the agar or slightly above it giving the plate a fluffy appearance.
Most contaminant molds will sporulate on the plate, giving a powdery, dusty, colored appearance.
Some fungi have tinted mycelia though, so if the whole mass of the organism is say, a pale tan, or yellowish, that is acceptable.
A perfectly happy and healthy sample:


Various contaminants:

As for your plates I'm afraid cant really tell well enough from the pictures to say if theyre contaminant or not.
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Edited by matsc (09/23/13 01:14 AM)
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OICU812
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Re: Growing mycelium on agar [Re: matsc]
#18879305 - 09/23/13 05:50 AM (10 years, 4 months ago) |
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Excellent example pics, natsc! Here is one more from my photos of Hericium erinaceus where the mycelium look like threads.
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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benny9922
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Re: Growing mycelium on agar *DELETED* [Re: OICU812]
#18879653 - 09/23/13 08:55 AM (10 years, 4 months ago) |
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Post deleted by benny9922Reason for deletion: I
-------------------- All you need in life is "health" and with that you can have anything!
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hidyn
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Re: Growing mycelium on agar [Re: benny9922]
#18885325 - 09/24/13 02:57 PM (10 years, 4 months ago) |
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Oh man, I'm only now just trying to work with agar, but those plates look ROUGH man! 
Are you trying to culture cubensis? Because whatever that is looks healthy, but I'd wager it's not P. Cubensis myc.
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