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OfflineHammburgler
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Registered: 08/13/13
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Injecting Stalled WBS with mutispore.
    #18874766 - 09/22/13 05:58 AM (10 years, 4 months ago)

So 2 weeks or so ago I took a BRF cake, broke it up and popped it into a 1/2 gallon jar of sterilized WBS. It colonized about 40% and I shook it up after about 4 days. I have noticed no significant growth since then. Last night I cooked up a new batch of WBS and a few BRF cakes and made about 50 ml of spore solution from some prints I took. I  used about 6cc on the stalled WBS jar. Any chance this will kick in some growth?


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OfflineBlackPeace
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Re: Injecting Stalled WBS with mutispore. [Re: Hammburgler]
    #18874783 - 09/22/13 06:08 AM (10 years, 4 months ago)

Hi,

I don't think it will work, if strong mycelium itself doesn't grow there, the chances that the spores themselves germinate there and save the world are very very very slim.

If your jars stall there it might be one of these reason:

-Not enough gas exchange
-Contamination
-Mycelium died due to excessive heat
-Too much water in the grains
-other reason

I bet on contamination since you used cakes to inoculate your sterilized grains.

Good luck


Edited by BlackPeace (09/22/13 06:09 AM)


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OfflineSupaThaRipper
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Re: Injecting Stalled WBS with mutispore. [Re: Hammburgler]
    #18874789 - 09/22/13 06:14 AM (10 years, 4 months ago)

You might have some bacterial contamination. Did you smell it?


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OfflineHammburgler
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Re: Injecting Stalled WBS with mutispore. [Re: SupaThaRipper]
    #18876037 - 09/22/13 02:00 PM (10 years, 4 months ago)

no i didnt smell it but i will after i get done eating in a few mins. I figured it prob wouldnt work but its worth a shot


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OfflineHammburgler
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Re: Injecting Stalled WBS with mutispore. [Re: Hammburgler]
    #18876068 - 09/22/13 02:07 PM (10 years, 4 months ago)

lol so i did the smell test and it smelled like something was fermenting in there


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InvisibleFrankHorrigan
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Re: Injecting Stalled WBS with mutispore. [Re: Hammburgler]
    #18876201 - 09/22/13 02:34 PM (10 years, 4 months ago)

Quote:

Hammburgler said:
lol so i did the smell test and it smelled like something was fermenting in there




:puke:

Best to practice good technique when dealing with sterile media :wink:


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