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Invisiblehostile_kid
living life

Registered: 11/04/11
Posts: 206
Loc: Chicago. IL Flag
About a week old Petri's .. IDK what they should look like! :x Green light? Red light?
    #18872883 - 09/21/13 04:24 PM (10 years, 5 months ago)

MS Orissa India :mushroom2::thumbup::thumbdown:






I know they are messy .  I wiped the sides and cleaned them up. after pouring. There has to be one that was done with adequate sterility. Unless all of these have the same contam . They were allowed to ungelatinize in the car accidently and a couple cooled at an angle with the agar on the inner sides. I taped the lids so theres not much air exchange since Im still moving. Can I put any of these in grain jars in a couple days or is another transfer smart. The growth is all white . Only one appears to have rhizo characteristics. the others are blobs. 


Edited by hostile_kid (09/21/13 04:32 PM)


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InvisibleinskiM
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Registered: 02/28/06
Posts: 5,752
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Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: hostile_kid]
    #18872904 - 09/21/13 04:30 PM (10 years, 5 months ago)

All of those dishes show bacterial contamination, how were they prepared?


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Invisiblehostile_kid
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Posts: 206
Loc: Chicago. IL Flag
Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: inski]
    #18872921 - 09/21/13 04:33 PM (10 years, 5 months ago)

terribly lol


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Invisiblehostile_kid
living life

Registered: 11/04/11
Posts: 206
Loc: Chicago. IL Flag
Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: hostile_kid]
    #18872939 - 09/21/13 04:36 PM (10 years, 5 months ago)

I forgot the purpose of STILL air . Not sterile air.

Agar was

8 gs potato flakes.
4 gs agar.
1 liter water

PC'd for a good amount of time. suffiecent.

Fucked up on the SA/GB with all the movement this is not suprising . You gotta slow down and do it right.


Edited by hostile_kid (09/21/13 04:37 PM)


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Invisiblehostile_kid
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Registered: 11/04/11
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Loc: Chicago. IL Flag
Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: hostile_kid]
    #18872978 - 09/21/13 04:44 PM (10 years, 5 months ago)

Even the blobs are bacterial?


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OfflineRogerRabbitM
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Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: hostile_kid]
    #18873056 - 09/21/13 05:03 PM (10 years, 5 months ago)

There is nothing there to save.  Toss that mess out.
RR


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Invisiblehostile_kid
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Registered: 11/04/11
Posts: 206
Loc: Chicago. IL Flag
Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: RogerRabbit]
    #18873932 - 09/21/13 09:26 PM (10 years, 5 months ago)

Thats a shame. Im definately gunna do some grain to agar on monday. That shall be my next test. :tongue2:


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Offlinethiotimoline
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Re: About a week old Petri's.. IDK what they should look like! :x Green light? Red light? [Re: hostile_kid]
    #18887255 - 09/24/13 11:08 PM (10 years, 4 months ago)

I've had plates that looked worse than that at a week and still eventually got germination and cleaned up the culture in a few transfers. That said, if spores aren't scare you should try again.

Might be a good idea to pour plates and leave them for a couple days before inoculating, so you don't waste spores on a plate that's already doomed.


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